Original Article | Published:

Anti-inflammation

Purified polysaccharide from Ginkgo biloba leaves inhibits P-selectin-mediated leucocyte adhesion and inflammation

Acta Pharmacologica Sinica volume 29, pages 499506 (2008) | Download Citation

This work was supported by the National Basic Research Program of China (No 2002 CB 513006) and the National Natural Science Foundation of China (No 30570928).

Abstract

Aim:

To investigate the anti-inflammatory mechanism of the polysaccharides of Ginkgo biloba leaves (PGBL) by inhibiting leucocyte adhesion.

Methods:

The rough PGBL were isolated and purified. The anti-inflammatory effects of purified PGBL (p-PGBL) were assayed by ear edema induced by xylol and the acute peritonitis model in mice. The effect of p-PGBL on inhibiting the interaction between P-selectin and its ligands was investigated by flow cytometry and flow chamber.

Results:

p-PGBL could effectively inhibit the acute inflammation in mice and interfere with the adhesion of HL-60 cells, a human leukaemia cell line, or neutrophils to P-selectin in static conditions, as well as the adhesion of neutrophils to Chinese hamster ovary cells expressing human P-selectin and human umbilical vein endothelial cells in flow conditions in a dose-dependant manner.

Conclusions:

p-PGBL can inhibit the inflammatory process through interfering with the interaction between P-selectin and its ligands.

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Author information

Affiliations

  1. Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China

    • Rui Fei
    • , Sheng Zheng
    • , Yan-guang Gao
    •  & Xian-lu Zeng
  2. Department of Cell Biology, School of Norman Bethune Medical Science, Jilin University, Changchun 130021, China

    • Rui Fei
  3. Department of Cardiology, Second Hospital, Jilin University, Changchun 130041, China

    • Yu Fei
  4. Department of Thoracic/Head and Neck Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston Texas 77030, USA

    • Hong-xia Sun

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DOI

https://doi.org/10.1111/j.1745-7254.2008.00765.x

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