Abstract
Aim:
To investigate the anti-inflammatory mechanism of the polysaccharides of Ginkgo biloba leaves (PGBL) by inhibiting leucocyte adhesion.
Methods:
The rough PGBL were isolated and purified. The anti-inflammatory effects of purified PGBL (p-PGBL) were assayed by ear edema induced by xylol and the acute peritonitis model in mice. The effect of p-PGBL on inhibiting the interaction between P-selectin and its ligands was investigated by flow cytometry and flow chamber.
Results:
p-PGBL could effectively inhibit the acute inflammation in mice and interfere with the adhesion of HL-60 cells, a human leukaemia cell line, or neutrophils to P-selectin in static conditions, as well as the adhesion of neutrophils to Chinese hamster ovary cells expressing human P-selectin and human umbilical vein endothelial cells in flow conditions in a dose-dependant manner.
Conclusions:
p-PGBL can inhibit the inflammatory process through interfering with the interaction between P-selectin and its ligands.
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This work was supported by the National Basic Research Program of China (No 2002 CB 513006) and the National Natural Science Foundation of China (No 30570928).
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Fei, R., Fei, Y., Zheng, S. et al. Purified polysaccharide from Ginkgo biloba leaves inhibits P-selectin-mediated leucocyte adhesion and inflammation. Acta Pharmacol Sin 29, 499–506 (2008). https://doi.org/10.1111/j.1745-7254.2008.00765.x
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DOI: https://doi.org/10.1111/j.1745-7254.2008.00765.x
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