Original Article | Published:

Antitumor Pharmacology

LY294002 induces p53-dependent apoptosis of SGC7901 gastric cancer cells

Acta Pharmacologica Sinica volume 29, pages 489498 (2008) | Download Citation

Project supported by the Health Fund of Jiangsu Province (H200719); Suzhou Social Development Fund (SS0517) and Jiangsu Six Talant Project (R2317344).

Abstract

Aim:

To study the effects of LY294002, an inhibitor of class I phosphatidylinositol 3-kinase (PI3K), on proliferation and apoptosis of SGC7901 gastric cancer cells.

Methods:

The MTT assay was used to determine the cytotoxic effects of LY294002. Cell cycle distribution was analyzed using flow cytometry and apoptosis was assessed using flow cytometry analysis after staining DNA with propidium iodide. Mitochondrial membrane potential was measured using the fluorescent probe JC-1. Expression of p53 and PUMA was determined using real-time RT-PCR and Western blotting analysis.

Results:

The viability of SGC7901 cells was significantly reduced by LY294002 treatment. Expression of p53 and PUMA was induced, and mitochondrial membrane potential collapsed after treatment with LY294002. LY294002 induced apoptotic cell death.

Conclusion:

Activation of the p53 pathway is involved in LY294002-induced SGC7901 cell death.

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Author information

Author notes

    • Chun-gen Xing
    •  & Bao-song Zhu

    Bao-song Zhu and Chun-gen Xing contributed equally to this article.

Affiliations

  1. Department of General Surgery, The Second Affiliated Hospital, Soochow University, Suzhou 215004, China

    • Chun-gen Xing
    • , Bao-song Zhu
    •  & Hui-hua Yao
  2. Department of Neurology, The Second Affiliated Hospital, Soochow University, Suzhou 215004, China

    • Hui-hui Liu
  3. Laboratory of Aging and Nervous Diseases, Soochow University School of Medicine, Suzhou 215123, China

    • Fang Lin
    • , Zhong-qin Liang
    •  & Zheng-hong Qin

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Correspondence to Bao-song Zhu or Zheng-hong Qin.

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DOI

https://doi.org/10.1111/j.1745-7254.2008.00770.x

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