Abstract
Aim:
To study whether gum mastic, a natural resin, can regulate maspin expression in prostate cancer cells, and further investigate the mechanisms involved in this regulatory system.
Methods:
RT-PCR and Western blotting were used to detect maspin expression at the transcriptional and translational levels. Reporter gene assay was used to investigate the effect of gum mastic on the maspin promoter. The binding activity of negative androgen-responsive element (ARE) and positive Sp1 element in the maspin promoter were studied by electrophoretic mobility shift assay.
Results:
Gum mastic induced maspin mRNA and protein expression, and the maspin promoter activity was enhanced with gum mastic treatment. Finally, gum mastic inhibited the ARE binding activity and increased the Sp1 binding activity in the maspin promoter.
Conclusion:
Gum mastic enhances maspin promoter activity by suppressing ARE binding activity and enhancing Sp1 binding activity, and the increased activity in the maspin promoter finally leads to the up-regulation of both its mRNA and protein levels.
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Project supported by the National Natural Science Foundation of China (No 30470820 and 30371564).
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He, Ml., Chen, Ww., Zhang, Pj. et al. Gum mastic increases maspin expression in prostate cancer cells. Acta Pharmacol Sin 28, 567–572 (2007). https://doi.org/10.1111/j.1745-7254.2007.00535.x
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DOI: https://doi.org/10.1111/j.1745-7254.2007.00535.x
Keywords
- gum mastic
- maspin expression
- ARE binding activity
- Sp1 binding activity
