Abstract
Aim:
To evaluate the neuroprotective effect and mechanisms of scutellarin (Scu) against PC12 cell injury after oxygen and glucose deprivation followed by reperfusion (OGD-Rep).
Methods:
Undifferentiated rat pheochromocytoma PC12 cells, exposed to oxygen and glucose deprivation followed by reperfusion (OGD-Rep), used as an in vitro model of ischemia/reperfusion. Cell survival was evaluated by diphenyltetrazolium bromide (MTT) assay and the amount of LDH release was determined using assay kits. [Ca2+]i was monitored using a fluorescent Ca2+-sensitive dye Fura-2 acetoxymethyl ester. Cell apoptosis was detected by a DNA ladder and by flow cytometric detection. The expression of protein kinase C (PKC)γ was determined using both RT-PCR and Western blotting. The translocation of PKCγ was assayed by subcellular fractionation and Western blotting.
Results:
OGD-Rep injury significantly elevated the level of LDH release, [Ca2+]i, mRNA expression and the translocation of PKCγ compared in the PC12 cells with those of the normal group. Scu (10-100 μmol/L) exerted a protective effect against OGD-Rep injury by reducing LDH release, [Ca2+]i, the percent of apoptosis, and the translocation of PKCγ.
Conclusion:
Scu inhibits the increase of [Ca2+]i and the activation of PKCγ, exerting protective effects against PC12 cell injury induced by OGD-Rep.
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Xu, W., Zha, Rp., Wang, Wy. et al. Effects of scutellarin on PKCγ in PC12 cell injury induced by oxygen and glucose deprivation. Acta Pharmacol Sin 28, 1573–1579 (2007). https://doi.org/10.1111/j.1745-7254.2007.00502.x
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DOI: https://doi.org/10.1111/j.1745-7254.2007.00502.x
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