Original Article | Published:

Gastrointestinal Pharmacology

Protective effects of heat shock protein70 induced by geranyl- geranylacetone in atrophic gastritis in rats

Acta Pharmacologica Sinica volume 28, pages 10011006 (2007) | Download Citation

Project supported by a grant from the National 863 Hi-Tech Program of China (No 2006AA02Z470).

Abstract

Aim:

To investigate the effect of geranylgeranylacetone (GGA) on the progression of atrophic gastritis in rats and its potential mechanism.

Methods:

Atrophic gastritis was induced in Sprague-Dawley rats with 0.1% ammonia solution, 60% ethanol, and 20 mmol/L deoxycholic acid for 24 weeks. Accompanied by the induction of atrophic gastritis, 200 mg/kg GGA was administered by oral gavage for 8 weeks (weeks 17-24). The histological changes in gastric mucosa were quantitated by the index of inflammation, the gastric mucosal thickness, and the amount of glands of 1 mm horizontal length in antrum. Endogenous heat shock protein (HSP)70 levels and distribution were determined by immunoblotting and immunohistochemistry in gastric mucosa.

Results:

GGA alleviated the pathological progression of atrophic gastritis with inflammation relief (inflammation index: 1.40 in the GGA group and 1.65 in the atrophic gastritis group) and glandular restoration (mucosal thickness and quantity of glands: 194.3 μm and 38.7 mm in the GGA group; 123.3 μm and 32.7 mm in the atrophic gastritis group; P<0.05). GGA significantly induced HSP70 synthesis (P<0.05). Moreover, quercetin, an inhibitor of HSP70 expression, aggravated the infiltration of inflammatory cells and glandular loss in the antrum.

Conclusion:

GGA prevented the progression of atrophic gastritis in rats via the induction of HSP70 expression.

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Affiliations

  1. Department of Gastroenterology and Zhejiang Key laboratory of Traditional Chinese Medicine (TCM), Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310012, China

    • Wei-li Liu
    • , Shu-jie Chen
    • , Yan Chen
    • , Lei-min Sun
    • , Wei Zhang
    • , Ya-min Zeng
    • , Tian-hua Zhou
    •  & Jian-min Si
  2. Department of Gastroenterology, Second Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou 310058, China

    • Yan Chen
  3. Center for Disease Modeling, Zhejiang University College of Medicine, Hangzhou 310058, China

    • Tian-hua Zhou

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DOI

https://doi.org/10.1111/j.1745-7254.2007.00589.x

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