Abstract
Aim:
To investigate the effect of geranylgeranylacetone (GGA) on the progression of atrophic gastritis in rats and its potential mechanism.
Methods:
Atrophic gastritis was induced in Sprague-Dawley rats with 0.1% ammonia solution, 60% ethanol, and 20 mmol/L deoxycholic acid for 24 weeks. Accompanied by the induction of atrophic gastritis, 200 mg/kg GGA was administered by oral gavage for 8 weeks (weeks 17-24). The histological changes in gastric mucosa were quantitated by the index of inflammation, the gastric mucosal thickness, and the amount of glands of 1 mm horizontal length in antrum. Endogenous heat shock protein (HSP)70 levels and distribution were determined by immunoblotting and immunohistochemistry in gastric mucosa.
Results:
GGA alleviated the pathological progression of atrophic gastritis with inflammation relief (inflammation index: 1.40 in the GGA group and 1.65 in the atrophic gastritis group) and glandular restoration (mucosal thickness and quantity of glands: 194.3 μm and 38.7 mm in the GGA group; 123.3 μm and 32.7 mm in the atrophic gastritis group; P<0.05). GGA significantly induced HSP70 synthesis (P<0.05). Moreover, quercetin, an inhibitor of HSP70 expression, aggravated the infiltration of inflammatory cells and glandular loss in the antrum.
Conclusion:
GGA prevented the progression of atrophic gastritis in rats via the induction of HSP70 expression.
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Project supported by a grant from the National 863 Hi-Tech Program of China (No 2006AA02Z470).
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Liu, Wl., Chen, Sj., Chen, Y. et al. Protective effects of heat shock protein70 induced by geranyl- geranylacetone in atrophic gastritis in rats. Acta Pharmacol Sin 28, 1001–1006 (2007). https://doi.org/10.1111/j.1745-7254.2007.00589.x
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DOI: https://doi.org/10.1111/j.1745-7254.2007.00589.x