Abstract
Aim:
To determine whether all-trans retinoic acid (atRA) acts to modulate angiotensin II (Ang II)-induced cardiac fibroblast cell growth and collagen secretion.
Methods:
Cultured neonatal rat cardiac fibroblasts (CF) were used in the experiment. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to detect cell growth of the CF; and immunocytochemistry and Western blotting were used to measure the production and secretion of collagen and the expression of transforming growth factor-β1 (TGF-β1) by the CF.
Results:
atRA (1 × 10−7 to 1 × 10−5 mol/L) inhibited the Ang II-induced increase in cell growth of CF (P<0.05). Ang II stimulated the secretion of collagen types I and III by the CF. This effect was blocked by AT1 receptor antagonist losartan (1 × 10−6 mol/L), but not by AT2 receptor antagonist PD123319 (up to 1 × 10−6 mol/L). Exposure of CF to atRA (1 × 10−5 mol/L) attenuated the Ang II-induced increase in the secretion of collagen types I and III (P<0.05). atRA (1 × 10−5 mol/L) also blocked the Ang II-induced increase in the expression of TGF-β1
Conclusion:
atRA inhibits the Ang II-induced increase in cell growth and collagen secretion of neonatal rat CF. The effect of atRA is possibly mediated by lowering the TGF-β1 level. These observations support the notion that atRA is a potential candidate for the prevention and therapy of cardiac remodeling.
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Project supported by the National Natural Science Foundation of China (No 39970241).
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He, Y., Huang, Y., Zhou, L. et al. All-trans retinoic acid inhibited angiotensin II-induced increase in cell growth and collagen secretion of neonatal cardiac fibroblasts. Acta Pharmacol Sin 27, 423–429 (2006). https://doi.org/10.1111/j.1745-7254.2006.00296.x
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DOI: https://doi.org/10.1111/j.1745-7254.2006.00296.x