Abstract
Aim:
To investigate the diverse pharmacological actions of astragaloside IV from the perspective of metabolic syndrome, and to investigate the effect of the drug on the pathogenesis of metabolic syndrome.
Methods:
Adipogenesis was used as an indicator of the effect of astragaloside IV on preadipocyte differentiation, and was measured by using an oil red O assay. Glucose uptake was determined by measuring the transport of [2-3H]-deoxyglucose into the cells. The concentrations of peroxisome proliferator-activated receptor-γ(PPARγ) and aP2 mRNA were determined by using reverse transcription-polymerase chain reaction. Apoptosis and viability loss of endothelial cells were detected by using flow cytometry and the WST-1 assay, respectively. Intracellular free Ca2+ was labeled with Fluo-3 AM and measured by using a laser scanning confocal microscope.
Results:
Astragaloside IV can significantly potentiate insulin-induced preadipocyte differentiation at concentrations of 3, 10, and 30 μg/mL, improve high glucose-induced insulin resistance in adipocytes at a concentration of 30 μg/mL, and prevent tumor necrosis factor (TNF)-α-induced apoptosis and viability loss at concentrations of 10 and 30 μg/mL, and 30 μg/mL, respectively, in endothelial cells. Furthermore, we found that these effects were partly due to the promotion of PPARγ expression and to the inhibition of abnormal TNF-α-induced intracellular free Ca2+ accumulation in endothelial cells.
Conclusion:
The diverse pharmacological actions of astragaloside IV can all be linked to metabolic syndrome pathogenesis. Our study provides a new insight into the mechanism by which astragaloside IV exerts its effect.
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Project supported by the Economic and Trade Commission of Zhejiang Province, the Key Laboratory of Chinese Medicine Screening, Exploitation and Medicinal Effectiveness Appraisal for Cardio-cerebral Vascular and Nervous System of Zhejiang Province, and the Key Laboratory for Biomedical Engineering of Ministry of China.
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Xu, Me., Xiao, Sz., Sun, Yh. et al. Effects of astragaloside IV on pathogenesis of metabolic syndrome in vitro. Acta Pharmacol Sin 27, 229–236 (2006). https://doi.org/10.1111/j.1745-7254.2006.00243.x
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DOI: https://doi.org/10.1111/j.1745-7254.2006.00243.x
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