Gastrointestinal Pharmacology

Effect of Oenanthe javanica flavone on human and duck hepatitis B virus infection



To study the antiviral effect of Oenanthe javanica flavones (OjF) on human hepatoma HepG2.2.15 culture system and duck hepatitis B virus (DHBV) infection.


(1) After incubation for 24 h, the 2.2.15 cells were treated with different concentrations of OjF for 12 d. The cell alteration was observed by microscope. The presence of HBsAg and HBeAg were measured using the enzyme immunoassay kit after 2.2.15 cells were treated with OjF for 9 d. (2) Ducklings infected with DHBV intravenously were divided into 5 groups and treated with OjF, acyclovir (ACV), and normal saline respectively for 10 d. All the ducklings were bled before, during, and after treatments at different times, and serum levels of DHBV-DNA were detected by a dot-blot hybridization assay.


(1) The 50% toxic concentration (TC50) of OjF was 2.28 g/L. The maximum nontoxic concentration (TC0) was 1.00 g/L. In nontoxic concentrations, OjF significantly inhibited HBsAg and HBeAg in 2.2.15 cells after 9 d of treatment (P<0.05, P<0.01). (2) The DHBV-DNA levels decreased significantly after the treatment with 0.50 and 1.00 g/kg of OjF (P<0.01). The inhibition of the peak of viremia was maximum at a dose of 1.00 g/kg and reached 54.3% on d 5 and 64.5% on d 10, respectively.


The results demonstrate that OjF is a strong inhibitor of HBsAg and HBeAg secretion in 2.2.15 cells and DHBV-DNA levels in the HBV-infected duck model.


  1. 1

    Huang ZM, Yang XB, Cao WB . Modern study and clinic application of Oenanthe javanica. Pharm J Chin PLA 2001; 17: 266–9.

    Google Scholar 

  2. 2

    Huang ZM, Zhang ZM, Yang XB, Cao WB . Study of Shui Qin on antihepatitis. Pharmacol Clin Chin Mat Med 1991; 7: 11–3.

    Google Scholar 

  3. 3

    Yang XB, Huang ZM, Cao WB . Effect of an aqueous extract from Oenanthe javanica on rat cardiovascular system. Chin Tradit Herb Drugs 1998; 10: 47–9.

    Google Scholar 

  4. 4

    Ji GJ, Cao WB, Huang ZM . Anti-arrhythmic effect of injections of SQ. Chin Pharm J 1990; 15: 134–8.

    Google Scholar 

  5. 5

    Zhang JZ, Cao WB, Yang XB, Huang ZM . Anti-anaphylactic effect of the decoction of SQ. Pharmacol Clin Chin Mat Med 1992; 8( suppl): 29–32.

    CAS  Google Scholar 

  6. 6

    Yang XB, Huang ZM, Cao WB . Antidiabetic effect of Oenanthe javanica flavone. Acta Pharmacol Sin 2000; 21: 239–42.

    PubMed  Google Scholar 

  7. 7

    Huang ZM, Yang XB, Cao WB . Effects of Qin ling ke li in the treatment of 90 patients with chronic hepatitis B. Pharm J Chin PLA 2001; 17: 41–4.

    CAS  Google Scholar 

  8. 8

    Huang ZM, Yang XB, Cao WB . Inhibition of shuiqin on DHBV in vitro. Chin Pharm J 1997; 32: 720–3.

    CAS  Google Scholar 

  9. 9

    Huang ZM, Yang XB, Cao WB . Study of inhibition of duck hepatitis B virus by extract from Oenanthe javanica. Chin Sci Bull 1997; 42: 1863–7.

    Google Scholar 

  10. 10

    Sha M, Cao A, Wang B . Determination of Hyperin in Sanguisorba officinalis L by high performance liquid chromatography. Chin J Chromatogr 1998; 16: 226–8.

    CAS  Google Scholar 

  11. 11

    Wu Y, Zhou SD, Li P . Determination of flavonoids in Hypericum perforatum by HPLC analysis. Acta Pharm Sin 2002; 37: 280–2.

    CAS  Google Scholar 

  12. 12

    Lamivudine Clinical Practice Group. Lamivudine treatment consensus from relative experts in 2004. Chin J Hepatol 2004; 12: 425–7.

  13. 13

    Hantz O, Perigaud C, Borel C, Jamard C, Zoulim F . The SATE pronucleotide approach applied to acyclovir Part II. Effect of bis(SATE)phosphotriester derivatives of acyclovir on duck hepatitis B virus replication in vitro and in vivo. Antiviral Res 1999; 40: 179–87.

    CAS  Article  Google Scholar 

  14. 14

    Liu J, Shen HM, Ong CN . Salvia miltiorrhiza inhibits cell growth and induces apoptosis in human hepatoma HepG2 cells. Cancer Lett 2000; 153: 85–93.

    CAS  Article  Google Scholar 

  15. 15

    Sells MA, Chen ML, Acs G . Production of hepatitis B virus particles in HepG2 cells transfected with cloned hepatitis B virus DNA. Proc Natl Acad Sci USA 1987; 84: 1005–9.

    CAS  Article  Google Scholar 

  16. 16

    Wu B, Wang CN, Xu JR . Effect of epidermal growth factor on cultured rat hepatocytes poisoned by CC14. Acta Pharmacol Sin 1997; 18: 176–9.

    CAS  Google Scholar 

  17. 17

    Liu J, Liu YP, Curtis DK . Protective effect of oleanolic acid against chemical-induced acute necrotic liver injury in mice. Acta Pharmacol Sin 1995; 16: 97–102.

    Google Scholar 

  18. 18

    Chen HY, Liu J, Zhu HF, Xu L . Action of acid-base extract of Oenanthe javanica on mice models of CCl4 hepatic injury. Pharm J Chin PLA 2001; 17: 22–4.

    Google Scholar 

  19. 19

    Xiong QB, Fan WZ, Tezuka YH . Hepatoprotective effect of Apocynum ventun and its active constituents. Planta Med 2000; 66: 127–33.

    CAS  Article  Google Scholar 

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Corresponding authors

Correspondence to Xin-bo Yang or Zheng-ming Huang.

Additional information

Project supported by the National New Drug Research Foundation of China (No 96-901-05-016).

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Wang, W., Yang, X., Liu, H. et al. Effect of Oenanthe javanica flavone on human and duck hepatitis B virus infection. Acta Pharmacol Sin 26, 587–592 (2005).

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  • Oenanthe javanica
  • flavones
  • hepatitis B virus
  • 2.2.15 cells
  • Duck hepatitis B virus
  • antiviral agents

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