Abstract
Aim:
To design the effective directed differentiation medium to differentiate bone marrow cells into hepatocyte-like cells.
Methods:
Bone marrow cells were cultured in the directed differentiation media including fibroblast growth factor-4 (FGF-4) and oncostatin M (OSM). Hepatocyte-like cells from directed differentiation of bone marrow cells were identified through cell morphology, RNA expressions by reverse transcriptase-polymerase chain reaction (RT-PCR), protein expressions by Western blot, and hepatocellular synthesis and metabolism functions by albumin ELISA, Periodic acid-Shiff staining and urea assay.
Results:
Some epithelial-like cells or polygonal cells appeared and increased in the course of the cell directed differentiation. Hepatocyte nucleur factor-3β (HNF-3β), albumin (ALB), cytokeratin 18 (CK18), transthyretin (TTR), glucose-6-phosphate (G-6-Pase), and tyrosine aminotransferase (TAT) mRNA were expressed in the course of the directed differentiation. The directed differentiated cells on d 21 expressed HNF-3β, ALB, and CK18 proteins. The directed differentiated cells produced albumin and synthesized urea in a time-dependent manner. They could also synthesize glycogen.
Conclusion:
Our differentiation media, including FGF-4 and OSM, are effective to differentiate bone marrow cells into hepatocyte-like cells, which could be used for hepatocyte resources for bioartificial liver or hepatocyte transplantation.
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Project supported by the National Natural Science Foundation of China (No 30371391) and the National Natural Science Foundation of Jiangsu Province, China (No BK2003008).
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Shi, Xl., Qiu, Yd., Li, Q. et al. Hepatocyte-like cells from directed differentiation of mouse bone marrow cells in vitro. Acta Pharmacol Sin 26, 469–476 (2005). https://doi.org/10.1111/j.1745-7254.2005.00093.x
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DOI: https://doi.org/10.1111/j.1745-7254.2005.00093.x
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