Abstract
Aim:
To investigate the effects of CpG-oligodeoxynucleotide (CpG-ODN) on the formation of macrophage foam cells and related gene expression.
Methods:
A gene expression profile was examined by microarray techniques, and mRNA expression was detected by reverse transcriptase polymerase chain reaction (RT-PCR). The cholesterol and cholesteryl ester contents of cells were determined by high performance liquid chromatography.
Results:
CD36, LPL, and Fcγ2b, which were related to lipid metabolism and the formation of macrophage foam cells, were upregulated after CpG-ODN stimulation. The mRNA expression related to the formation of foam cells was confirmed by semiquantitative RT-PCR. Moreover, histochemical analysis confirmed that lipid deposits inside cells increased after CpG-ODN treatment. However, using flow cytometry, we found that CpG-ODN had no effect on the expression of membrane receptors.
Conclusion:
CpG-ODN up-regulated the expression of genes in macrophage foam cell formation.
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Project supported by the National Natural Science Foundation of China (No 30370741).
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Li, K., Wan, B., Hu, Zl. et al. Effects of CpG-ODN on gene expression in formation of foam cells. Acta Pharmacol Sin 26, 1359–1364 (2005). https://doi.org/10.1111/j.1745-7254.2005.00167.x
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DOI: https://doi.org/10.1111/j.1745-7254.2005.00167.x