Abstract
Aim:
To search genes sensitivity to the anti-cancer drugs navelbine (NVB) and docetaxel (DOC) in small-cell lung cancer (SCLC) and non-small-cell lung cancer (NSCLC) cell strains.
Methods:
The sensitivity of 4 strains of SCLC and 6 strains of NSCLC to NVB and DOC was evaluated using the MTT assay. The expression of 1291 sensitive-related genes to the anti-cancer drugs in 10 lung cancer cell strains was measured using cDNA macroarrays and the relationship was analyzed.
Results:
In total, there were 56 (r≥0.4) genes sensitive to NVB and DOC. For NVB: 36 genes were sensitive to NVB, 20 co-expressed genes between the SCLC+NSCLC set and the NSCLC set; 27 expressed genes and 7 specially expressed genes in the SCLC+NSCLC set; and 29 expressed genes and 9 specially expressed genes in the NSCLC set. For DOC, 50 genes were sensitive to DOC, 12 co-expressed genes between the SCLC+NSCLC set and the NSCLC set; 24 expressed genes and 12 specially expressed genes in the SCLC+NSCLC set; and 38 expressed genes and 26 specially expressed genes in the NSCLC set. The genes sensitive to NVB and DOC in lung-cancer cell stains were mainly divided into the following 4 categories: signal transduction molecules, cell factors, transcription factors and metabolism-related enzymes and inhibitors.
Conclusions:
There were obvious differences in genes related to NVB and DOC between SCLC and NSCLC cell strains, but the same as categories of function.
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Project supported by a Repatriation Grant from the Foundation of Science Bureau of Harbin, China (2003 AFLXJ009), also funded by Overseas Scholar Grant from the Department of Education of HeiLongJiang Province, China (1055HZ020).
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Cai, L., Dong, Hy. & Sui, Gj. Effects of navelbine and docetaxel on gene expression in lung cancer cell strains. Acta Pharmacol Sin 26, 1017–1024 (2005). https://doi.org/10.1111/j.1745-7254.2005.00142.x
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DOI: https://doi.org/10.1111/j.1745-7254.2005.00142.x
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