Figure 2

From: Cannabinoid 2 receptor induction by IL-12 and its potential as a therapeutic target for the treatment of anaplastic thyroid carcinoma

Figure 2

(a) Effect of WIN-55,212–2 and JWH133 on apoptosis in ARO, ARO/vector, ARO/cannabinoid 2 receptor (CB2), and ARO/interleukin (IL)-12 cells. The cells were treated with different concentrations of WIN-55,212–2 or JWH133 for 24 h and stained with annexin V and propidium iodide (PI). Apoptotic cells were quantified by flow cytometry analysis and presented as a percentage of cell population. The data are expressed as means±s.e.m. of four separate experiments. (b) Representative results of flow cytometry analysis of apoptotic cells. ARO and ARO/CB2 cells were treated with 1 μM WIN-55,212–2 or 2 μM JWH133 for 24 h, and stained with annexin V and PI. Significant increase in apoptosis was observed in ARO/CB2 cells treated with WIN-55,212–2 or JWH133 as compared to ARO cells.