Table 1 PTX and VCR enhance the oncolytic effect of Ad5 in H460 cells in MTT assays

From: Paclitaxel and vincristine potentiate adenoviral oncolysis that is associated with cell cycle and apoptosis modulation, whereas they differentially affect the viral life cycle in non-small-cell lung cancer cells

  LC 50 (PFU./ml) a
  2 days 5 days
  Ad Ad+10PTX Ad+50PTX Ad Ad+10PTX Ad+50PTX
Mean 30.0 28.0 20.0 0.17 0.025 0.005
SF 1.0 1.1 1.5 1.0 6.8 34.0
  Ad Ad+10VCR Ad+50VCR Ad Ad+10VCR Ad+50VCR
Mean 28.5 25.0 6.0 0.17 0.02 0.004
SF 1.0 1.1 4.8 1.0 8.5 42.5
  1. Abbreviations: MOI, multiplicity of infection; MTT, 3-[4,5-dimethylthiazol]-2,5-diphenyltetrazolium bromide; PTX, paclitaxel; SF, sensitization factor; VCR, vincristine.
  2. Results obtained at different MOI and combined with 10 or 50 nM drug after 2 and 5 days post-infection are indicated.
  3. aValues are the mean (n=3)±s.d., which was always less than 10% of the mean.