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  • Research Article
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Human respiratory uptake of chloroform and haloketones during showering


Inhalation is an important exposure route for volatile water contaminants, including disinfection by-products (DBPs). A controlled human study was conducted on six subjects to determine the respiratory uptake of haloketones (HKs) and chloroform, a reference compound, during showering. Breath and air concentrations of the DBPs were measured using gas chromatography and electron capture detector during and following the inhalation exposures. A lower percentage of the HKs (10%) is released from shower water to air than that of chloroform (56%) under the experiment conditions due to the lower volatility of the HKs. Breath concentrations of the DBPs were elevated during the inhalation exposure, while breath concentrations decreased rapidly after the exposure. Approximately 85–90% of the inhaled HKs were absorbed, whereas only 70% of the inhaled chloroform was absorbed for the experiment conditions used. The respiratory uptake of the DBPs was estimated using a linear one-compartment model coupled with a plug flow stream model for the shower system. The internal dose of chloroform normalized to its water concentration was approximately four times that of the HKs after a 30-min inhalation exposure. Approximately 0.3–0.4% of the absorbed HKs and 2–9% of the absorbed chloroform were expired through lung excretion after the 30-min exposure. The inhalation exposure from a typical 10–15 min shower contributes significantly to the total dose for chloroform in chlorinated drinking water but only to a moderate extent for HKs.

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  • Chinery R.L., and Gleason A.K. A compartmental model for the prediction of breath concentration and absorbed dose of chloroform after exposure while showering. Risk Anal 1993: 13(1): 51–62.

    Article  CAS  Google Scholar 

  • Clemens M., and Scholer H.F. Halogenated organic compounds in swimming pool water. Zentralblatt Hygiene Umweltmedizin 1992: 193(1): 91–98 (in German).

    CAS  Google Scholar 

  • Comroe J.H. Physiology of Respiration, 2nd edn. Year Book Medical Publishers, Chicago, IL, 1974.

    Google Scholar 

  • Corley R.A., Mendrala A.L., Smith F.A., Staats D.A., Gargas M.L., Conolly R.B., Anderson M.E., and Reitz R.H. Development of a physiologically based pharmacokinetic model for chloroform. Toxicol Appl Pharmacol 1990: 103(3): 512–527.

    Article  CAS  Google Scholar 

  • Curieux F., Marzin D., and Erb F. Study of the genotoxic activity of five chlorinated propanones using the sos chromotest, the ames-uctuation test and the newt micronucleus test. Mutat Res 1994: 341: 1–15.

    Article  Google Scholar 

  • Gargas M.L., Burgess R.J., Voisard D.E., Cason G.H., and Andersen M.E. Partition coefficients of low-molecular-weight volatile chemicals in various liquids and tissues. Toxicol Appl Pharmacol 1989: 98(1): 87–99.

    Article  CAS  Google Scholar 

  • Gordon S.M., Kenny D.V., and Kelly T.J. Continuous real time breath analysis for the measurement of half lives of expired volatile organic compounds. J Expos Anal Environ Epidemiol 1992: 1: 41–54.

    Google Scholar 

  • Gordon S.M., Wallace L.A., Pellizzari E.D., and O’Neill H.J. Human breath measurements in a clean air chamber to determine half lives for volatile organic compounds. Atmos Environ 1988: 22: 2165–2170.

    Article  CAS  Google Scholar 

  • Hansch C., Leo A., and Hoekman D. Exploring QSAR: Hydrophobic, Electronic, and Steric Constants (ACS Professional Reference Book). American Chemical Society, Washington, DC, 1995.

    Google Scholar 

  • Heinicke K., Wolfarth B., Winchenbach P., Biermann B., Sehmid A., Huber G., Friedmann B., and Schmidt W. Blood volume and hemoglobin mass in elite athletes of different disciplines. Int J Sports Med 2001: 22(7): 504–512.

    Article  CAS  Google Scholar 

  • Jo W.K., Weisel C.P., and Lioy P.J. Chloroform exposure and the health risk associated with multiple uses of chlorinated tap water. Risk Anal 1990a: 10: 581–585.

    Article  CAS  Google Scholar 

  • Jo W.K., Weisel C.P., and Lioy P.J. Routes of chloroform exposure and body burden from showering with chlorinated tap water. Risk Anal 1990b: 10: 575–580.

    Article  CAS  Google Scholar 

  • Krasner S.W., MeGuire M.J., Jacongelo J.G., Patania N.L., Reagan K.M., and Aieta E.M. The occurrence of disinfection by-products in US drinking water. J AWWA 1989: 81(8): 41–53.

    Article  CAS  Google Scholar 

  • Pinheiro J.C., and Bates D.M. Mixed-effects Models in S and S-PLUS. Springer, New York, 2000.

    Book  Google Scholar 

  • Pleil J.D., and Lindstrom A.B. Exhaled human breath measurement method for assessing exposure to halogenated volatile organic compounds. Clin Chem 1997: 43(5): 723–730.

    CAS  PubMed  Google Scholar 

  • Raymer J.H., Pellizzari E.D., Thomas K.W., and Cooper S.D. Elimination of volatile organic compounds in breath after exposure to occupational and environmental microenvironments. J Expos Anal Environ Epidemiol 1991: 1(4): 439–451.

    CAS  Google Scholar 

  • Raymer J.H., Thomas K.W., and Cooper S.D. A device for sampling of human alveolar breath for the measurement of expired volatile organic compounds. J Anal Toxicol 1990: 14(6): 337–344.

    Article  CAS  Google Scholar 

  • Rook J.J. Formation of haloforms during chlorination of natural waters. Water Treat Exam 1974: 23: 234–243.

    Google Scholar 

  • Sheiner L.B., and Beal S.L. Evaluation of methods for estimating population pharmacokinetics parameters. I. Michaelis–Menten model: routine clinical pharmacokinetic data. J Pharmacokinet Biopharm 1980a: 8(6): 553–571.

    Article  CAS  Google Scholar 

  • Sheiner L.B., and Beal S.L. Evaluation of methods for estimating population pharmacokinetics parameters. II. Biexponential model: experimental pharmacokinetic data. J Pharmacokinet Biopharm 1980b: 9(5): 635–651.

    Article  Google Scholar 

  • US EPA. Exposure Factors Handbook, Vol. I: Review Draft. EPA/600/R-95/002Ba. U.S. Environmental Protection Agency, Washington, DC, 1996.

  • Vinegar A., Williams R.J., Fisher J.W., and McDougal J.N. Dose-dependent metabolism of 2,2-dichloro-1,1,1-triuoroethane: a physiologically based pharmacokinetic model in the male Fischer 344 rat. Toxicol Appl Pharmacol 1994: 29(1): 103–113.

    Article  Google Scholar 

  • Wallace L.A. Human exposure and body burden for chloroform and other trihalomethanes. Crit Rev Environ Sci Technol 1997: 27(2): 113–194.

    Article  CAS  Google Scholar 

  • Wallace L.A., Nelson W.C., Pellizzari E.D., and Raymer J.H. Uptake and decay of volatile organic compounds at environmental concentrations: application of a four-compartment model to a chamber study of five human subjects. J Expos Anal Environ Epidemiol 1997: 7: 141–163.

    CAS  Google Scholar 

  • Wallace L.A., Pellizzari E.D., and Gordon S.M. A linear model relating breath concentrations to environmental exposures: application to a chamber study of four volunteers exposured to volatile organic chemicals. J Expos Anal Environ Epidemiol 1993: 3: 75–102.

    CAS  Google Scholar 

  • Weisel C.P., and Jo W.K. Ingestion, inhalation, and dermal exposures to chloroform and trichloroethene from tap water. Environ Health Perspect 1996: 104(1): 48–51.

    Article  CAS  Google Scholar 

  • Weisel C.P., Jo W.K., and Lioy P.J. Utilization of breath analysis for exposure and dose estimates of chloroform. J Expos Anal Environ Epidemiol Suppl 1992: 1: 55–69.

    Google Scholar 

  • Weisel C.P., Xu X., and Trabaris M. Inhalation and dermal exposure to the DBPs: haloacetic acids, halopropanones, haloacetonitriles and chloral hydrate. In: Joint International Society of Exposure Analysis/International Society for Environmental Epidemiology Annual Meeting, August 2002 Vancouver, Canada, 2002: P. 145.

  • Xu X. Dermal and Inhalation Exposure to Disinfection By-products in “Drinking Water”, PhD thesis, Rutgers University, New Brunswick, NJ, 2002.

    Google Scholar 

  • Xu X., Mariano T., Laskin J.D., and Weisel C.P. Percutaneous absorption of tri-halomethanes, haloacetic acids and haloketones. Toxicol Appl Pharmacol 2002: 184: 19–26.

    Article  CAS  Google Scholar 

  • Xu X., and Weisel C.P. Inhalation exposure to haloacetic acids and haloketones during showering. Environ Sci Technol 2003: 37(3): 569–576.

    Article  CAS  Google Scholar 

  • Yu R., and Weisel C.P. Measurement of benzene in human breath associated with an environmental exposure. J Expos Anal Environ Epidemiol 1996: 6(1): 261–277.

    CAS  Google Scholar 

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This research was funded by the United States Environmental Protection Agency (U.S. EPA) Research Foundation (#GR825953-01-0). This presentation has not been subjected to the Agency's review and therefore does not necessarily reflect the views of the Agency. Clifford P. Weisel is supported in part by the NIEHS Center for Excellence Grant (ES05022-06).

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Correspondence to Clifford P Weisel.

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Xu, X., Weisel, C. Human respiratory uptake of chloroform and haloketones during showering. J Expo Sci Environ Epidemiol 15, 6–16 (2005).

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