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Measurement of bisphenol A levels in human urine

Journal of Exposure Science & Environmental Epidemiology volume 11, pages 323–328 (2001)Cite this article

Abstract

We report a new approach for assessing human exposure to bisphenol A (BPA) by measuring BPA in urine after enzymatic deglucuronidation. This method involves addition of 13C 12-labeled BPA, enzymatic deconjugation, solid-phase extraction, and derivatization with pentafluorobenzyl bromide. The product of the derivatization is separated by gas chromatography followed by mass spectrometric detection using negative chemical ionization and selected ion monitoring. Using this analysis method, urine samples fortified with both a constant level of labeled BPA and a range of unlabeled BPA levels (0.27–10.6 ng/ml) demonstrated constant percentage recovery. In addition, a range of urine sample volumes (0.25–10.0 ml) with constant amounts of added internal standard produced a linear response (r2=0.99). The method limit of detection was 0.12 ng/ml. This method was validated by duplicate analyses using gas chromatography coupled to a high-resolution mass spectrometer.

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Abbreviations

BPA:

bisphenol A

EI+:

positive electron ionization

GC/HRMS:

gas chromatography coupled to high-resolution mass spectrometric detection

GC/MS:

gas chromatography coupled to mass spectrometric detection

Hg:

mercury

m/z:

mass to charge ratio

NCI:

negative chemical ionization

SPE:

solid-phase extraction

TMS:

trimethylsilyl

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Acknowledgements

This work was supported in part by a grant-in-aid from the Ministry of Health and Welfare of Japan. Brand names are given for reference and do not constitute endorsement.

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Authors and Affiliations

  1. National Center for Environmental Health, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, USA

    JOHN W BROCK, JOHN R BARR, VINCENT L MAGGIO, SAM R GRAISER & LARRY L NEEDHAM

  2. Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan

    YOSHIHIRO YOSHIMURA & HIROYUKI NAKAZAWA

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  1. JOHN W BROCK
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Correspondence to JOHN W BROCK.

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BROCK, J., YOSHIMURA, Y., BARR, J. et al. Measurement of bisphenol A levels in human urine. J Expo Sci Environ Epidemiol 11, 323–328 (2001). https://doi.org/10.1038/sj.jea.7500174

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