Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Urinary F2-isoprostanes are poor prognostic indicators for the development of bronchopulmonary dysplasia

Abstract

Objective:

Oxygen toxicity is thought to contribute to the development of bronchopulmonary dysplasia (BPD). Oxidant injury leads to formation of F2-isoprostanes (F2-IsoP). We hypothesized that urinary excretion of the stable metabolite of F2-IsoP, 8-iso-PGF2α, would be higher in infants who develop BPD than those who did not.

Methods:

Forty infants <30-weeks gestational age (GA) were enrolled, 24 infants with BPD and 16 without BPD. Urine specimens were collected weekly and stored at −80°C until analyzed. Urinary 8-iso-PGF2α was measured by gas chromatography/mass spectrometry (GC-MS) and normalized to creatinine excretion.

Results:

GA and birth weight (BW) were lower in infants who developed BPD than those who did not. Urinary 8-iso-PGF2α levels in the first or third weeks of age were not significantly different between the two groups.

Conclusion:

Urinary excretion of 8-iso-PGF2α in early postnatal life in preterm infants is not correlated with the development of BPD.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Northway Jr WH, Rosan RC, Porter DY . Pulmonary disease following respirator therapy of hyaline-membrane disease. Bronchopulmonary dysplasia. N Engl J Med 1967; 276: 357–368.

    Article  Google Scholar 

  2. Bancalari E, del Moral T . Bronchopulmonary dysplasia and surfactant. Biol Neonate 2001; 80 (Suppl 1): 7–13.

    Article  CAS  Google Scholar 

  3. Jobe AH, Bancalari E . Bronchopulmonary dysplasia. Am J Respir Crit Care Med 2001; 163: 1723–1729.

    Article  CAS  Google Scholar 

  4. Frank L, Groseclose EE . Preparation for birth into an O2-rich environment: the antioxidant enzymes in the developing rabbit lung. Pediatr Res 1984; 18: 240–244.

    Article  CAS  Google Scholar 

  5. O'Donovan DJ, Fernandes CJ . Free radicals and diseases in premature infants. Antioxid Redox Signal 2004; 6: 169–176.

    Article  CAS  Google Scholar 

  6. Morrow JD, Roberts LJ . The isoprostanes: their role as an index of oxidant stress status in human pulmonary disease. Am J Respir Crit Care Med 2002; 166 (12 Part 2): S25–30.

    Article  Google Scholar 

  7. Pratico D, Basili S, Vieri M, Cordova C, Violi F, Fitzgerald GA . Chronic obstructive pulmonary disease is associated with an increase in urinary levels of isoprostane F2alpha-III, an index of oxidant stress. Am J Respir Crit Care Med 1998; 158: 1709–1714.

    Article  CAS  Google Scholar 

  8. Montuschi P, Corradi M, Ciabattoni G, Nightingale J, Kharitonov SA, Barnes PJ . Increased 8-isoprostane, a marker of oxidative stress, in exhaled condensate of asthma patients. Am J Respir Crit Care Med 1999; 160: 216–220.

    Article  CAS  Google Scholar 

  9. Ciabattoni G, Davi G, Collura M, Iapichino L, Pardo F, Ganci A et al. In vivo lipid peroxidation and platelet activation in cystic fibrosis. Am J Respir Crit Care Med 2000; 162 (4 Part 1): 1195–1201.

    Article  CAS  Google Scholar 

  10. Cracowski JL, Cracowski C, Bessard G, Pepin JL, Bessard J, Schwebel C et al. Increased lipid peroxidation in patients with pulmonary hypertension. Am J Respir Crit Care Med 2001; 164: 1038–1042.

    Article  CAS  Google Scholar 

  11. Morrow JD, Zackert WE, Yang JP, Kurhts EH, Callewaert D, Dworski R et al. Quantification of the major urinary metabolite of 15-F2t-isoprostane (8-iso-PGF2alpha) by a stable isotope dilution mass spectrometric assay. Anal Biochem 1999; 269: 326–331.

    Article  CAS  Google Scholar 

  12. Stier C, Schweer H, Jelinek J, Watzer B, Seyberth HW, Leonhardt A . Effect of preterm formula with and without long-chain polyunsaturated fatty acids on the urinary excretion of F2-isoprostanes and 8-epi-prostaglandin F2alpha. J Pediatr Gastroenterol Nutr 2001; 32: 137–141.

    Article  CAS  Google Scholar 

  13. Ahola T, Fellman V, Kjellmer I, Raivio KO, Lapatto R . Plasma 8-isoprostane is increased in preterm infants who develop bronchopulmonary dysplasia or periventricular leukomalacia. Pediatr Res 2004; 56: 88–93.

    Article  CAS  Google Scholar 

  14. Barden A, Beilin LJ, Ritchie J, Croft KD, Walters BN, Michael CA . Plasma and urinary 8-iso-prostane as an indicator of lipid peroxidation in pre-eclampsia and normal pregnancy. Clin Sci (Lond) 1996; 91: 711–718.

    Article  CAS  Google Scholar 

  15. Roberts LJ, Morrow JD . Measurement of F(2)-isoprostanes as an index of oxidative stress in vivo. Free Radic Biol Med 2000; 28: 505–513.

    Article  CAS  Google Scholar 

  16. Bessard J, Cracowski JL, Stanke-Labesque F, Bessard G . Determination of isoprostaglandin F2alpha type III in human urine by gas chromatography-electronic impact mass spectrometry. Comparison with enzyme immunoassay. J Chromatogr B Biomed Sci Appl 2001; 754: 333–343.

    Article  CAS  Google Scholar 

  17. Proudfoot J, Barden A, Mori TA, Burke V, Croft KD, Beilin LJ et al. Measurement of urinary F(2)-isoprostanes as markers of in vivo lipid peroxidation – a comparison of enzyme immunoassay with gas chromatography/mass spectrometry. Anal Biochem 1999; 272: 209–215.

    Article  CAS  Google Scholar 

  18. Comporti M, Signorini C, Leoncini S, Buonocore G, Rossi V, Ciccoli L . Plasma F2-isoprostanes are elevated in newborns and inversely correlated to gestational age. Free Radic Biol Med 2004; 37: 724–732.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors thank Dr Jason Morrow from Vanderbilt University for his assistance with the GC-MS protocol.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to S D Reuter.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Reuter, S., O'Donovan, D., Hegemier, S. et al. Urinary F2-isoprostanes are poor prognostic indicators for the development of bronchopulmonary dysplasia. J Perinatol 27, 303–306 (2007). https://doi.org/10.1038/sj.jp.7211684

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.jp.7211684

Keywords

Search

Quick links