Abstract
A fourth recently discovered isozyme of sn-glycerol-3-phosphate dehydrogenase (GPDH-4) in Drosophila melanogaster is shown to be a translational product of the Gpdh transcript which contains exons 1 through 7. This transcript was also found in two other Drosophila species, D. busckii and D. virilis. In contrast to D. melanogaster and D. busckii, the Gpdh transcript containing exons 1–6 is absent in D. virilis adults. The reason for this difference between D. virilis and the two other species is intriguing but remains elusive. We have ruled out the possibility that a replacement of an amino acid residue in exon 7 played any role in generating this interspecific variation.
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Introduction
In Drosophila melanogaster the enzyme sn-glycerol-3-phosphate dehydrogenase (GPDH, EC 1.1.1.8) is present in three isozymic forms. GPDH-1 is predominant in the adult flight muscle and involved in energy production for flight, whereas GPDH-3 is localized mainly in the fat body tissue, both in larvae and adult abdomens, and is postulated to serve a dual function regulating the cytoplasmic NAD+/ NADH ratio and providing sn-glycerol-3-phosphate as a precursor for lipid biosynthesis (Bewley & Miller, 1979). GPDH-2 is found in small amounts in those adult tissues where both GPDH-1 and GPDH-3 are expressed. As the functional GPDH molecule is a dimer (Collier et al., 1976) it is possible that GPDH-2 is a heterodimer composed of one GPDH-1 subunit and one GPDH-3 subunit (Wright & Shaw, 1969).
Gpdh transcripts are generated by alternative ways of processing the pre-mRNA (Cook et al., 1988). The D. melanogaster Gpdh gene has eight exons, a single promoter and multiple polyadenylation signals (Bewley et al., 1989; von Kalm et al., 1989). There are two alternate 3′ exons — exon 7 and exon 8. The transcript containing exons 1 through to 6 codes for GPDH-3 and the transcript containing exons 1–6, skipping exon 7 and including exon 8 encodes GPDH-1 (Bewley et al., 1989; von Kalm et al., 1989). The product of translation of the transcript containing exons 1–7 has not been identified, but it has been suggested that this transcript might be coding for a novel isozyme GPDH-4 (Wilanowski et al., 1995).
The amino acid sequence of GPDH has been obtained from a variety of organisms, and shown to be highly conserved over evolutionary time (Bewley et al., 1989). In fruit fly species this conservation extends to the nucleotide sequence (Kwiatowski et al., 1997) and isozyme patterns (Colgan, 1992). Despite this apparent congruity in the patterns of Gpdh variation, few data are available on the extent to which the regulation of the gene's expression is conserved.
For example, the GPDH electrophoretic phenotypes of D. virilis and D. busckii appear to be very similar to that of D. melanogaster (Fig. 1). However, Tominaga et al. (1992) were able to clone only two kinds of Gpdh cDNA from a D. virilis adult cDNA library — one contained exons 1–7 and the second exons 1–6 and 8. This result suggests that, unlike in D. melanogaster, the GPDH-3-encoding transcript might not be expressed in D. virilis adults. Recently, Wilanowski et al. (1995) have shown that in the D. melanogaster GpdhAKy33 allele the insertion of the blood retrotransposon into the noncoding part of exon 7 induces the production of a novel isozyme, GPDH-4, and causes an over-expression of the Gpdh transcript which contains exons 1–7. The comparison between D. virilis and D. melanogaster poses a number of questions regarding the evolution of Gpdh regulation. We have attempted to answer some of these questions by using P-element mediated transformation in D. melanogaster of a construct containing exons 1–7 and by investigating Gpdh expression in D. virilis and D. busckii.
Materials and methods
Drosophila strains
Wild-type strains of D. virilis and D. busckii were provided by Allan R. Lohe. The D. melanogaster mutant allele GpdhAKy33 was isolated from a natural population at Cardwell, Queensland, Australia, in 1988 (Gibson et al., 1991). A D. melanogaster stock bearing a Gpdh null allele, GpdhnJH231, on a balancer chromosome CyO (Bewley & Lucchesi, 1977) was obtained from the Bowling Green Stock Centre. Df (2L)GdhA is a deficiency that spans the Gpdh locus (Lindsley & Grell, 1968). Flies heterozygous GpdhnJH231/Df(2L)GdhA have no detectable GPDH activity and very poor viability.
Gpdh gene constructs and transformation
Two overlapping genomic clones which cover the Gpdh locus, pDm59 and pDm60, were provided by the late Glenn C. Bewley (Cook et al., 1986). From these clones a construct, pGpdh(1–7), was made to include exons 1–7, the promoter and the 3′ sequences necessary for polyadenylation (Bartoszewski, 1996). The region located downstream of the HindIII site in the noncoding part of exon 6 was replaced by a PCR-amplified fragment covering the entire exon 7. The fragment extends from 91 nucleotides upstream of exon 7 to 48 nucleotides upstream of exon 8. The splice sites between exons 6 and 7 are retained in the pGpdh(1–7) construct, but the entire exon 8 is removed (Fig. 2). The polyadenylation signals in exon 6 are located 3′ to the HindIII site so that they too are removed.
It is known that there is a competition between the reactions of splicing and cleavage-polyadenylation and if the competition from one pre-mRNA processing pathway is eliminated then the other pathway is exclusively followed by default (Peterson et al., 1994). In the pGpdh(1–7) construct effective polyadenylation can occur only in exon 7 as the only polyadenylation signals retained in this construct are those in exon 7. Consequently exon 7 is expected to be spliced to exon 6 as the splice sites in exons 6 and 7 are intact and the competing polyadenylation signals of exon 6 are removed. Thus the only mature Gpdh transcript that can be expressed from this construct is the one which contains exon 7 (Fig. 2). The pGpdh(1–7) construct is not able to produce GPDH-1 or GPDH-3.
P-element mediated transformation, with a wild-type white gene as marker, was used to obtain transformants. The pGpdh(1–7) construct was subcloned into the pW8 vector (Klemenz et al., 1987) and transformed into D. melanogaster embryos by microinjection (Bartoszewski & Gibson, 1994); the recipient strain was homozygous for white eye colour. All the experimental procedures involved in making the construct and in transforming it into D. melanogaster have been described in detail by Bartoszewski (1996).
GPDH electrophoresis
GPDH electrophoresis on cellulose acetate membranes with a buffer at pH 6.6 was performed according to Gibson et al. (1986).
Rapid amplification of cDNA ends (RACE) and identification of RACE products
The RACE analysis of the 3′ ends of Gpdh transcripts was carried out as previously described (Symonds et al., 1995). A primer specific to exon 5 of the D. virilis Gpdh gene, JG119 (5′-CGGGCAAGACAATCGAAGAT-3′) was based on the sequence published by Tominaga et al. (1992). For the study of D. busckii we used the JG11 primer (5′-AGAAGCTGCAGGGCCCACCCA-3′), which is specific to exon 5 of the D. melanogaster Gpdh gene. The RACE products obtained were subcloned into pBluescript as before (Wilanowski et al., 1995) and identified by sequencing with the ABI Prism dye terminator cycle sequencing reaction kit (Perkin-Elmer) according to the manufacturer's instructions.
Results
The pGpdh(1–7) construct was expressed in the D. melanogaster flies made heterozygous GpdhnJH231/Df (2L)GdhA so that they lack endogenous GPDH activity (Bewley & Lucchesi, 1977). A number of separately produced transformant lines were obtained but each displayed identical GPDH electrophoretic phenotypes. The phenotype was compared with those of the GpdhF and GpdhAKy33 alleles. As predicted, the transgenic flies express only one GPDH isozyme, which has the same electrophoretic mobility as GPDH-4 in GpdhAKy33 (Fig. 3). This result very strongly supports the hypothesis that this isozyme, named GPDH-4, is a product of translation of a Gpdh transcript containing exons 1 through 7 (Wilanowski et al., 1995).
Three RACE product bands were obtained from D. virilis: two both from adults and larvae, with one band common to both life stages (Fig. 4). All the fragments visible in Fig. 4 have been cloned and sequenced. The band expressed in larvae and adults was found to represent a transcript containing exon 7, that is, the equivalent of the GPDH-4-encoding transcript in D. melanogaster. These data also show that the transcript containing exons 1–6 — the homologue of the GPDH-3-encoding transcript — is present in D. virilis but only in larvae, whereas the equivalent of the D. melanogaster transcript coding for GPDH-1 is unique to the adult stage of D. virilis.
In D. busckii, initial RACE experiments gave several products (Fig. 5). Upon sequencing, only two of them — indicated in the figure — were found to correspond to Gpdh transcripts; other bands resulted from nonspecific amplification. For example, GenBank searching showed that the ≈1 kb RACE fragment represented a transcript encoding acetyl-coenzyme A carboxylase (EC 6.4.1.2). The sensitivity of the RACE method was low probably because of the use of the JG11 primer (Symonds et al., 1995), which was based on the D. melanogaster Gpdh sequence (Bewley et al., 1989; von Kalm et al., 1989) and had two mismatches in comparison with the D. busckii Gpdh sequence (R. Spencer Wells, personal communication).
Nevertheless, the RACE analysis of D. busckii indicated that the homologue of the GPDH-1-encoding transcript was unique to the adult stage and the one coding for GPDH-3 was present both in adults and larvae. This experiment failed to detect a transcript containing exon 7 in D. busckii. To test further whether this transcript was present in D. busckii, we used a more direct approach. A D. melanogaster exon-7-specific primer, JG99 (5′-GACGGCATGATGGACGTATC-3′), was used with JG11 to PCR amplify only the fragment unique to the transcript containing exons 1–7. By this method we detected this transcript both in adults and larvae of D. busckii (data not shown). Our findings are summarized in Table 1.
Discussion
The GPDH electrophoretic phenotypes of D. virilis and D. busckii appear to resemble that of D. melanogaster (Fig. 1). However, the RACE analyses (Figs 4 and 5) showed that although the pattern of Gpdh transcription is similar in D. busckii and D. melanogaster, it differs from that in D. virilis. In D. melanogaster, both from adult and larval cDNA samples, three RACE products are obtained that correspond to the transcripts encoding GPDH-1, GPDH-3 and GPDH-4, respectively (Symonds et al., 1995; Wilanowski et al., 1995). The most significant difference in D. virilis is the lack of the GPDH-3-encoding transcript in adults. The transcript coding for GPDH-4, however, was found in D. virilis adults (Fig. 4) which suggests that in this species the least anodal isozymic band might be GPDH-4. We base this hypothesis on a comparison between the GPDH electrophoretic phenotype (Fig. 1) and the outcome of the RACE analysis (Fig. 4). In D. virilis the most anodal GPDH staining band and the GPDH-1-encoding transcript are both unique to adults, consequently we argue that this band represents the GPDH-1 isozyme. Thus it is reasonable to assume that the least anodal GPDH staining band in D. virilis adults is encoded by the only other Gpdh transcript expressed at this stage of development and hence it is the homologue of the GPDH-4 isozyme in D. melanogaster. The middle band in D. virilis might then be a heterodimer composed of one GPDH-1 subunit and one GPDH-4 subunit. Similarly, the middle band in D. melanogaster and D. busckii adults might be a mixture of heterodimers made of one GPDH-1 subunit and one GPDH-3 or GPDH-4 subunit (Wilanowski et al., 1995).
These findings prompt a question about the basis of the variation in Gpdh expression in the Drosophila genus. A comparison of Gpdh sequences obtained from D. melanogaster (Bewley et al., 1989; von Kalm et al., 1989) and D. virilis (Tominaga et al., 1992) revealed that the splice sites in exons 6 and 7 are perfectly conserved but the sequences adjacent to polyadenylation signals in these exons are very different in D. melanogaster and D. virilis. This result provides new support to the hypothesis originally formulated by Cook et al. (1988) that the polyadenylation site selection is the regulated event in Gpdh expression.
The change of isozymic patterns could have been triggered by a mutation in exon 7 which is translated only in GPDH-4 (Bewley et al., 1989; von Kalm et al., 1989). There is one amino acid difference between D. virilis and D. melanogaster in this exon. It is located at residue 353, which is phenylalanine in D. virilis but serine in D. melanogaster (Tominaga et al., 1992). Drosophila busckii is reputed to be the only other Drosophila species that has the same mutation in exon 7 as D. virilis (Wells, 1996). However, in D. busckii the transcripts coding for GPDH-3 and GPDH-4 are expressed both in adults and larvae, which is different from D. virilis (Table 1). It remains possible that some other mutation in the GPDH protein is associated with the shift in the GPDH isozymic pattern. There are seven amino acid replacements in D. virilis in comparison with the D. melanogaster GpdhS allele (Tominaga et al., 1992). Unfortunately, as many as three of them are unique to D. virilis (Wells 1996) so this supposition is very difficult to test.
The data overall emphasize that seemingly similar phenotypes can be produced by different regulatory patterns in different species. Further studies on the expression of the Gpdh transcripts in various species of Diptera should shed light on this important evolutionary phenomenon.
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Acknowledgements
We thank R. Spencer Wells for providing us with the unpublished nucleotide sequence of the coding region of the Gpdh gene from D. busckii, and an anonymous referee for very useful suggestions.
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Wilanowski, T., Yoong, S., Bartoszewski, S. et al. Expression of the GPDH-4 isozyme of sn-glycerol-3-phosphate dehydrogenase in three Drosophila species. Heredity 81, 390–395 (1998). https://doi.org/10.1038/sj.hdy.6883890
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DOI: https://doi.org/10.1038/sj.hdy.6883890
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