Criollo cacao (Theobroma cacao ssp. cacao) was cultivated by the Mayas over 1500 years ago. It has been suggested that Criollo cacao originated in Central America and that it evolved independently from the cacao populations in the Amazon basin. Cacao populations from the Amazon basin are included in the second morphogeographic group: Forastero, and assigned to T. cacao ssp. sphaerocarpum. To gain further insight into the origin and genetic basis of Criollo cacao from Central America, RFLP and microsatellite analyses were performed on a sample that avoided mixing pure Criollo individuals with individuals classified as Criollo but which might have been introgressed with Forastero genes. We distinguished these two types of individuals as Ancient and Modern Criollo. In contrast to previous studies, Ancient Criollo individuals formerly classified as ‘wild’, were found to form a closely related group together with Ancient Criollo individuals from South America. The Ancient Criollo trees were also closer to Colombian-Ecuadorian Forastero individuals than these Colombian-Ecuadorian trees were to other South American Forastero individuals. RFLP and microsatellite analyses revealed a high level of homozygosity and significantly low genetic diversity within the Ancient Criollo group. The results suggest that the Ancient Criollo individuals represent the original Criollo group. The results also implies that this group does not represent a separate subspecies and that it probably originated from a few individuals in South America that may have been spread by man within Central America.
Theobroma cacao (2n = 2x = 20) is a tree native to humid tropical regions of the northern part of South America and, according to some reports, of Central America (Miranda, 1962). Indeed, there is still some controversy about the origin and domestication of cacao.
Some authors believe that cacao was introduced into Central America. Although the first centre of domestication and culture has been identified as Central America, Van Hall (1914) stated that the most probable origin of cacao is the region of the Orinoco and Amazon basins, in the valleys of their tributaries. Cheesman (1944) considered that the centre of origin of cacao was the Upper Amazon near the Colombian-Ecuadorian border, on the eastern flanks of the Andes. Cheesman (1944) argued that although cacao has been cultivated in Mexico and Central America for over 2000 years, no truly wild populations were present in this region, suggesting that cacao was introduced into Central America and Mexico. Schultes (1984) hypothesized that once cacao had spread throughout the Amazon Valley, it could have dispersed along two routes: one leading north and the other west. In this way, the domestication of cacao occurred in South America and then spread to Central America and Southern Mexico, carried by migrating indians (Schultes, 1984).
Cuatrecasas (1964) suggested separate simultaneous origin in South and Central America, a hypothesis that has been supported by most subsequent authors (Cope, 1976; Wood and Lass, 1985; Gómez-Pompa et al, 1990; Laurent et al, 1994; De la Cruz et al, 1995; Whitkus et al, 1998). Based on the great morphological diversity observed in Central as well as in South America, Cuatrecasas (1964) proposed that North and South American cacao populations developed into two forms, separated by the Panama Isthmus. Both populations evolved independently and are recognised as separate subspecies (T. cacao ssp. cacao and T. cacao ssp. sphaerocarpum; Cuatrecasas, 1964). Furthermore, Cuatrecasas (1964) hypothesized that wild plants from the Lacandona rainforest from Mexico were possible ancestors of domesticated cacao. Separate simultaneous origin and domestication in both Central and South America has been suggested for the common bean, Phaseolus vulgaris (Velasquez and Gepts, 1994).
The subspecies proposed by Cuatrecasas correspond to the two morphogeographic groups proposed by Cheesman (1944); T. cacao ssp. cacao and T. cacao ssp. sphaerocarpum are synonyms for the Criollo and Forastero groups, respectively. A third hybrid group originating from crosses between Criollo and Forastero was called Trinitario. The Forastero group is composed of very diverse populations with different geographic origins: Upper Amazon, Lower Amazon, Orinoco and the Guianas. Forastero trees are also identified according to pod morphology, eg the Amelonado type characterised by a melon shape.
Criollo cacao was cultivated in Latin America during the pre-Columbian and colonial period, and had a higher quality than Forastero types, but with a low vigour and yield (Cheesman, 1944). Since 1825, it has been steadily replaced by more disease resistant and productive Trinitario clones in countries such as Venezuela (Pittier, 1935).
This paper describes our work on the genetic structure of Criollo, and its genetic relationships with other cacao populations, based on molecular analyses of a large sample of Criollo from several Latin American countries.
Materials and methods
Samples were classified as Ancient Criollo, Modern Criollo, Trinitario, Lower Amazon Forastero, Orinoco Forastero, French Guiana Forastero, Upper Amazon Forastero and hybrids with at least one Upper Amazon Forastero parent (Table 1). A complete list of individuals used in the study is available upon request.
Ancient Criollo individuals consisted of trees showing the morphological traits described by Cheesman (1944) for the Criollo group, sampled from places where gene flow between Criollo and Trinitario or Forastero trees was absent or limited due to the improbability of Trinitario or Forastero introductions. Most samples came from trees on old or abandoned farms and in private gardens in difficult to access towns. In Mexico, samples of Ancient Criollo were also collected in the Lacandona rainforest where wild trees had previously been reported and studied (Miranda, 1962; Cuatrecasas 1964; De la Cruz et al, 1995; Whitkus et al, 1998), and in places where Mayan peoples cultivated cacao; in the Yucatan sinkholes (Gómez-Pompa et al, 1990; De la Cruz et al, 1995; Whitkus et al, 1998) and on the Pacific coast of Mexico (Lopez-Mendoza, 1987). Samples from the Belizian rainforest associated with Mayan ruins (Mooledhar et al, 1995) were also included in the analysis.
Modern Criollo individuals were defined as those showing the morphological traits described by Cheesman (1944) for the Criollo group, but sampled on modern farms or on farms where significant introductions of Trinitario or Forastero were suspected. This class also included material from germplasm collections in Costa Rica, Côte d’Ivoire, Mexico, Venezuela and France. ‘Modern Criollo’ represents the genotypes studied as Criollo in previous biochemical and molecular studies (Lanaud, 1987; Bekele and Bekele, 1998; Ronning and Schnell, 1994; Laurent et al, 1995; N’Goran et al, 1994; De la Cruz et al, 1995; Lerceteau et al, 1997; Whitkus et al, 1998).
Trinitario, Forastero clones from Orinoco, French Guiana, the Lower and Upper Amazon (LAF and UAF) and hybrids with at least one Upper Amazon Forastero parent, were studied to compare the structure of their genetic diversity to that of Ancient and Modern Criollo.
For RFLP analyses, DNA was isolated using ultracentrifugation in a caesium chloride-ethidium bromide gradient as described in Lanaud et al (1995). For microsatellite analysis, DNA isolation was performed as described in Risterucci et al (2000); after total resuspension, a QIAGEN genomic-tip was used to purify the DNA according to the manufacturer’s instructions.
All RFLP procedures were conducted as described previously (Lanaud et al, 1995). EcoRI and HindIII were used for genomic DNA restriction.
Seventeen cDNA and eight genomic DNA probes, chosen for their coverage of the genetic map of T. cacao (Lanaud et al, 1995), were used to study 283 individuals.
Sixteen microsatellites (Lanaud et al, 1999) were used to study the genetic diversity of 102 individuals.
Primers were end-labelled with γ−33P ATP and PCR amplification was performed in a MJ Research PTC 100 thermal cycler, in 20 μl of reaction mix containing 10 ng cacao DNA, 0.2 mM dNTP mix, 2 mM MgCl2, 50 mM KCl, 10 mM Tris-HCl (ph 8.3), 0.2 μM primer (5′ end labelled with (γ−33P ATP) and 1 unit Taq polymerase (Eurobio). The samples were denatured at 94°C for 4 min, and subjected to 32 repeats of the following cycle: 94°C for 30 sec, 46°C or 51°C for 1 min and 72°C for 1 min. After adding 20 μl of loading buffer (98% formamide, 10 mM EDTA, bromophenol blue, xylene cyanol), the mix was denatured at 92°C for 3 min and 3 μl of each sample were loaded in a 5% polyacrylamide gel with 7.5 M urea and electrophoresed in 0.5% TBE buffer at 55 W for 1 h 40 min. The gel was dried for 30 min at 80°C and exposed overnight to X-ray film (Fuji RX).
RFLP alleles observed after hybridization with 25 probes were scored. Microsatellite allele sizes were scored by comparing PCR product lengths to the sequence of the genomic clone from which primers were designed. Repeat number variance was calculated as the sum of the squares of the difference between the mean number of repeats at the locus and the repeat number for each allele multiplied by its frequency in the population.
The genetic diversity statistic (Nei, 1978), the mean number of alleles per locus, the percentage of polymorphic loci at the 95% significance level, and the observed heterozygosity were calculated using Genetix 4.0 software. Hybrid clones were not included in the calculation of these statistics for the Forastero group.
For RFLP and microsatellite data the shared allele distance (DAS) (Chakraborty and Jin, 1993) was calculated. This distance is equal to 1 minus the proportion of shared alleles: DAS = 1 − (a/2n), where a is the number of common alleles to individuals i and j and n the number of loci studied. This distance was computed by averaging the values over all available loci between two individuals.
Multidimensional scaling (MDS) was performed on a DAS matrix calculated from RFLP data using the MDS procedure of SAS (version 6.12) software.
To determine the relatedness between Criollo and Forastero individuals a neighbour-joining (N-J) tree (Saitou and Nei, 1987) was constructed from the shared allele distance between individuals obtained from microsatellite data. The DAS estimation, N-J tree construction and bootstrapping procedures were conducted using a computer program kindly provided by Jean-Marie Cornuet and Sylvain Piry (Laboratoire de Modelisation et Biologie Evolutive, INRA, Montpellier, France). Hybrid genotypes were excluded from the analysis.
Microsatellite and RFLP analyses were compared using a Mantel test on DAS matrices of 92 individuals. The Mantel test was performed after 1000 permutations on the order of individuals in one of the matrices using the software Genetix 4.0.
After hybridization with 25 probes, 66 alleles were detected. The unbiased gene diversity (Nei, 1978) was higher for Forastero than for Ancient Criollo (Table 2). The average number of alleles per locus was highest for the Forastero group, as were the percentage of polymorphic loci and the observed heterozygosity. This latter parameter was very low (0.002) for the Ancient Criollo group, indicating almost complete homozygosity as a genetic characteristic of this group (Table 2).
All Ancient Criollo individuals (n = 92) mapped in the right half of the MDS plane, with a cluster of homogeneous trees and several near-identical individuals in the third quadrant (Figure 1). Only eight multilocus genotypes were observed among the Ancient Criollo individuals (three of them are represented superimposed on Figure 1), whereas each Forastero clone had a unique RFLP genotype. Some Ancient Criollo individuals that shared identical RFLP genotypes were members of different morphotypes or from diverse geographical areas (Venezuela, Colombia, Nicaragua, Belize and Mexico). For instance, some cacao trees from the Lacandona rainforest had identical RFLP profiles to genotypes putatively cultivated by the Mayas (found in the sinkholes of Yucatan, on the Pacific Coast of Mexico and in Belize) as well as to individuals cultivated today in South America.
Modern Criollo individuals are superimposed onto Trinitario (Figure 1). Furthermore they are distributed continuously from Ancient Criollo in the third quadrant to Amelonado Forastero in the first. Only three Modern Criollo and one Trinitario are found in the fourth quadrant of the plane of the MDS. These individuals show introgression of alleles specific to Upper Amazon Forastero trees from Peru (see Table 3). Most of the hybrids with at least one Upper Amazon Forastero parent were represented in the fourth quadrant.
The statistics for the genetic diversity of Modern Criollo were similar to those of Trinitario (Table 2). The unbiased genetic diversity and the observed heterozygosity of Modern Criollo were higher than those of Ancient Criollo. Similarities between Modern Criollo and Trinitario are to be expected given that the distinction, based on morphological traits, between the two types is subjective.
The 16 microsatellite loci detected 150 alleles. Despite the increased number of alleles, the genetic diversity of the Ancient Criollo group (0.04) observed from microsatellite data was still very low compared to that observed for the Forastero group (0.78; Table 4). Within geographic regions, the genetic diversities of 13 individuals from Peru and five individuals from Colombia-Ecuador were similarly high (0.70). The observed heterozygosity was 0.00 for the Ancient Criollo and 0.34 for Forastero. The average number of alleles per locus was the highest for the Forastero group (8.69 and 1.19 for Forastero and Criollo, respectively), as was the percentage of polymorphic loci (1.00 vs 0.06). Allele size variance was much higher for Forastero than for Ancient Criollo (14.02 vs 0.08).
Within the Ancient Criollo individuals (n = 41), only six genotypes were found among different morphotypes or across diverse geographic zones, as was observed for the RFLP analysis (Figure 2, group A). In this tree, Ancient Criollo individuals were more related to Colombian-Ecuadorian Forastero individuals (EBC5, EBC 6, EBC 10, Lcteen 37 and Lcteen 355) than the latter are to some Peruvian, French Guiana or Lower Amazon Forastero individuals. The clustering pattern reflects the geographic origin of individuals. Medium to high bootstrap values support this last result (Figure 2).
In general, for the microsatellite analysis, Modern Criollo had similar values for genetic diversity statistics to those obtained for the Trinitario group (Table 4).
As observed in the RFLP analysis, Modern Criollo and Trinitario microsatellite alleles were also present in Ancient Criollo individuals and Amelonado clones. This finding, combined with the evidence from genetic diversity statistic (similar values obtained for both Modern Criollo and Trinitario), supports the hybrid character of Modern Criollo.
Comparison of RFLP and microsatellite analyses
The 25 RFLP probes detected 66 alleles, whilst 16 microsatellites loci detected 150 alleles. DAS values were slightly lower for RFLP than for microsatellite data; mean distance between individuals was 0.41 (s.d. 0.21) and 0.64 (s.d. 0.30), respectively. The relationship between individuals was very similar for both techniques. For example, Upper Amazon individuals from Colombia and Ecuador were more related to Ancient Criollo for DAS values than to other Forastero individuals using both RFLP and microsatellite markers (Figures 1 and 2). For the mantel test of DAS RFLP and microsatellite matrices, a Pearson correlation coefficient of 0.9 (r = 0.9) was obtained with a probability of one for dependence between the two matrices.
The analyses of RFLP and microsatellite markers presented here shed new light on the patterns of genetic diversity and genetic relationships amongst T. cacao populations. Both techniques yielded equivalent results, despite the number of alleles detected per locus being significantly higher for microsatellites than for RFLPs. The correlation between RFLP and microsatellite DAS matrices was highly significant. There is no apparent effect of the higher mutation rate for microsatellites (Dallas, 1992; Dietrich et al, 1992) on the determination of relatedness between cacao individuals. Congruency between RFLP and microsatellite diversity patterns has also been observed for other species (Pejic et al, 1998; Desplanque et al, 1999).
Both our RFLP and microsatellite analyses clearly distinguished Ancient Criollo individuals from Modern Criollo (Ancient Criollo individuals introgressed with Forastero genes). It is important to note that previous studies using isozymes (Lanaud, 1987; Ronning and Schnell, 1994), RFLP (Laurent et al, 1994; Lerceteau et al, 1997), and RAPD markers (N’Goran et al, 1994; Lerceteau et al, 1997) have analysed what are defined here as Modern Criollo (usual representatives of the Criollo group). The present study is the first to show that the two types (Modern and Ancient) can be distinguished, and this was made possible by using a sample that avoided mixing pure Criollo individuals with individuals classified as Criollo but likely to have been introgressed with Forastero genes. Thus, individuals classified as Ancient Criollo constitute the true Criollo group comprised of cacao genotypes cultivated before the introduction of Forastero individuals to cacao plantations. Natural hybridisation between these two groups later gave rise to the appearance of Modern Criollo or Trinitario.
De la Cruz et al (1995) and Whitkus et al (1998) found that cacao trees from the Lacandona rainforest and ‘Criollo’ from germplasm collections could be clearly distinguished. These studies used dominant markers (RAPD), and the relatedness between what was called Criollo and what was called ‘wild’ (individuals from the Lacandona rainforest) was not clearly established, in contrast to the present study. They too found that samples from the Lacandona rainforest and those from the sinkholes of the Yucatan were clearly different.
In the present study, we analysed seven individuals from three sinkholes from Yucatan (near the towns of Yaxcaba, Tixcacaltuyub and Chechmil). Using 25 RFLP probes and 16 microsatellites we found that these seven individuals shared an identical genotype. Contrary to the findings of De la Cruz et al (1995) and Whitkus et al. (1998) little differentiation was observed between individuals from Yucatan and the Lacandona rainforest: The genotype found in the seven individuals from Yaxcaba, Tixcacaltuyub and Chechmil was also found in nine out of 13 individuals from the Lacandona rainforest.
The origin of the cacao cultivated by the Mayas
Very low diversity (Figure 1, Tables 2 and 3) was found within the Ancient Criollo group comprising individuals from the Lacandona rainforest, even though some of them were obtained from distant sites. It has been suggested that the Criollo group originated in the Lacandona rainforest where such trees are apparently present in the wild state (Miranda, 1962; Cuatrecasas, 1964; Gómez-Pompa et al, 1990 and De la Cruz et al, 1995). This hypothesis does not agree with the results present here. Indeed, a wild population should exhibit levels of genetic diversity similar to that observed within geographic areas (for example, in Peru or Colombia-Ecuador, Table 4). This was not the case; very low diversity associated with high homozygosity was observed in Central America (including the Lacandona rainforest). Moreover, cacao from the Lacandona rainforest was found to be identical at a molecular level to individuals putatively cultivated by the Mayas (those found in sinkholes, the Pacific Coast of Mexico and Belize) and to individuals from the regions of southwestern Venezuela and northeastern Colombia. Therefore, the population consisting of trees found at the Lacandona rainforest should neither be considered wild nor as originating from this region. Another element that must be considered is the absence of palynological evidence of the presence of Theobroma in the forests of Chiapas before the human colonization. Pollen of genera belonging to the modern vegetation of Chiapas has been observed from Tertiary deposits, but not Theobroma or related genera such as Herrania (Graham, 1999). In addition, in the Lacandona rainforest, where material was sampled, vestiges of the Mayan civilization were frequently found. Thus, the presence of Criollo cacao trees in the Lacandona rainforest may be a remnant of cacao cultivation by the Mayas.
Our results contradict Cuatrecasas’ (1964) hypothesis that Criollo is a separate subspecies that evolved independently to South American populations in Central America, and suggest rather that the Criollo group had a South American origin. If Cuatrecasas’ hypothesis is true, all wild Forastero individuals should be clustered independently of Criollo in the analysis of genetic relatedness between individuals (Figure 2). In contrast, Ancient Criollo individuals are more related to Forastero from Colombia and Ecuador (EBC 5, EBC 6, EBC 10, Lcteen 37 and Lcteen 355) than the latter are to other Forastero individuals from French Guiana, the Orinoco, the Lower Amazon or some from Peru (ie, GU154, Matina 1-6, Venc 4, PA 107). Consequently, the Criollo group does not form a separate subspecies (ssp. cacao) from the one comprising individuals from South America (ssp. sphaerocarpum). Moreover, since microsatellite mutations tend to change allelic size by small amounts (Schlötterer and Tautz, 1992), the low allele size variance found for Ancient Criollo (0.08) compared to Forastero (14.02) also indicates a recent origin for this group.
Classification within the species
Since genetic distances between some Forastero individuals are equivalent to that observed between some Forastero and Ancient Criollo, a classification of cacao based on two main populations (Criollo and Forastero) has no genetic base. Indeed, the classification based on Criollo and Forastero mentioned by Cheesman (1944) and first proposed by Morris (1882) was simply based on the terms used by the Venezuelan cacao producers of the central coastal zone. At the time of Morris, the terms Criollo and Forastero were employed to distinguish the local cultivated trees (with a specific pod morphology) from the introduced foreign material.
Evolution and domestication
Allopatric divergence of cacao populations is suggested by the clustering pattern of individuals (Figure 2). Clear divergence of cacao from specific origins such as French Guiana and Ecuador has been reported (Lanaud, 1987; Laurent et al, 1994; Sounigo et al, 1996; Lerceteau et al, 1997). Although a reduced number of Forastero genotypes for each South American region were studied (Table 3), RFLP and microsatellite alleles, specific (allelic frequency higher than 0.05) to groups of individuals from different geographic areas were identified. Furthermore, mitotypes as well as rDNA alleles specific to different geographic origins have been observed (Laurent et al, 1993a, b). Collection expeditions in Amazonia (Allen and Lass, 1983; Young, 1994) revealed striking differences in morphology among populations from different river tributaries or other topographic features. Patterns of genetic diversity in other Amazonian plant species, eg Hevea brasiliensis (Seguin et al, 1999) and Elaeis oleifera (Barcelos, 1998), have also been reported as being associated with streams and explained according to ‘refuge’ theory (Simpson and Haffer, 1978; Haffer, 1982). The geological changes on which the ‘refuge’ theory is based could also explain divergence among T. cacao populations (including the population at the origin of Criollo individuals). Isolated cacao populations in constricted forest ‘refuges’, possibly in contracted gallery forest along scattered tributaries, could have survived during the adverse climatic conditions that occurred during the Quaternary period. These populations could then have evolved independently into different variants prior to a subsequent phase of forest expansion (Lanaud, 1987; Young, 1994). Stepwise founder events over repeated cycles of forest contraction and expansion could have then led to the loss of much natural genetic diversity in Criollo prior to domestication.
Subsequently, bottlenecks could have occurred during domestication. The intervention of man through selection during cultivation could also have reduced the effective number of individuals of the original Criollo population. People would then have been able to fix and maintain the extreme phenotypes that could have appeared due to mutations in a few genes, and spread the crop into Central America. Some cacao types may have been of special interest to people and therefore selected through collection, maintenance and use. Indeed, such extremely different phenotypes as Porcelana and Pentagona (one very smooth and the other very rough) contrast strongly with other pod types. The Pentagona or Lagarto type, for example, has the finest pod cortex, increasing the bean weight to pod weight ratio and facilitating the extraction of the beans from the fruit. Characteristic traits of Criollo trees such as the sweet pulp of its beans and the fact that it needs less fermentation, could be seen as targets of selection by man during more than 1500 years of cultivation.
The results of the present study, combined with the evidence presented above, uphold the theories of Van Hall (1914), Cheesman (1944) and Schultes (1984), that cacao originated in South America, and was later introduced by man in Central America.
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This study was partly financed by the International Plant Genetic Resources Institute (IPGRI) through the 1998 Vavilov-Frankel Fellowship, by the Consejo Nacional de Ciencia y Tecnología of Venezuela (CONICIT) by the Fundación para el Desarrollo de la Ciencia y la Tecnología del Estado Aragua (FUNDACITE-ARAGUA, Venezuela) and by the Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD, France).
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Motamayor, J., Risterucci, A., Lopez, P. et al. Cacao domestication I: the origin of the cacao cultivated by the Mayas. Heredity 89, 380–386 (2002). https://doi.org/10.1038/sj.hdy.6800156
- Theobroma cacao L.
- genetic diversity
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