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Macular pigment in the human retina: histological evaluation of localization and distribution



Clinical investigations have demonstrated variation in both the peak optical density and the spatial distribution of macular pigment. To confirm these impressions histologically, the present study examined the distribution of macular pigment in the human retina.

Materials and Methods

The macular retina of 11 donor eyes of different ages (28–91years) were examined histologically on 100 μm vibratome sections directly, without further staining. Measurements were made in two dimensions: (1) adding the number of macular sections with visible macular pigment, and (2) direct measurement of the extension of macular pigment in the foveolar section, which visibly contained the most macular pigment.


The measurements with two methods demonstrated good correlation. The macula demonstrated a variation in the spatial extension of the visible macular pigment between 200 and 900 μm diameter around the centre of the fovea, which was also found when direct measurements were taken. There was no correlation with the donor age. The main location of macular pigment was in the layer of the fibres of Henle in the fovea and in the inner nuclear layer at the parafoveal site.


Histologically, a wide variation of the spatial distribution of macular pigment was found that confirms clinical observations. The primary localization of human macular pigment is in the inner retinal layers.


Oxidative damage of the central retina caused by retinal irradiation especially by blue light is thought to be an important factor in the development of age-related macular degeneration (AMD).1, 2, 3 To reduce these damaging processes several antioxidative and protective mechanisms exist in different layers of the central retina.4, 5 Besides antioxidative vitamins like vitamins A, C, and E,3, 6, 7, 8, 9, 10, 11, 12, 13 carotenoids such as lutein and zeaxanthin exist in the macula and are visible macroscopically as macular pigment.14, 15, 16, 17, 18, 19 These substances have two different antioxidative functions in the macula. First they absorb blue light in the inner retina and second they act as free radical scavengers in the photoreceptors.20, 21

In clinical psychophysical studies a relatively homogeneous spatial distribution of macular pigment has been proposed.22, 23, 24, 25 This observation was supported in a histological evaluation of the macular pigment in monkeys concluding a uniform distribution of macular pigment in the inner retina without variation between animals.21, 26, 27 However, in recent studies in humans using motion photometry and autofluorescence imaging methods a wide variation was observed in both the foveal peak concentration of macular pigment and in its spatial distribution.28, 29, 30 The aim of the present histological study was to investigate the localization and spatial distribution of macular pigment in the central human retina and to analyze any interindividual variation.

Materials and methods

To investigate the localization and distribution of macular pigment in the central human macula, the retinas of 11 human donor eyes of different age were examined. The eyes with no evident ophthalmic disorder were obtained from the Eye Bank at the Institute of Ophthalmology, London, Great Britain. The corneas had been removed for transplantation within 48 h of death. The donors ranged in age from 28 to 91 years. No other information about the donors was available for example concerning their refractive state or race. Our research adhered to the tenets of the Declaration of Helsinki. The globes were immersed in 4% paraformaldehyde for approximately 4 days and hemidisected. A 7 × 7 mm macula specimen was taken with a razorblade and the central retina was separated from the RPE-choroid-sclera complex and the optic disc. In accordance with the method described by Snodderly,26 the retinas were embedded in gelatine 25% (w/v) gels and sectioned with a vibratome. Consecutive sections of 100 μm (5% variation) thickness were collected.

These consecutive sections were examined unstained. The presence of yellow macular pigment and its localization was analysed by light microscopy immediately after the preparation of the sections, because oxidative processes resulted in a rapid fading of the visible macular pigment at room temperature.

Measurements were estimated in two dimensions: The analysis of macular pigment was performed in consecutive sections and the number of sections with visible yellow macular pigment was recorded and used to calculate the extent of pigment in one direction using the knowledge that each section was approximately 100 μm thick (Figure 1). The measurement of the size of yellow macular pigment visible in the central foveolar section of each specimen provides a second estimate of the spatial extension of macular pigment perpendicular to the first.

Figure 1

The central human fovea shown as graphics. The vertical lines indicate the localization of consecutive 100 μm vibratome sections.

Images were also acquired through narrow band pass filters at 460 and 530 nm, the spectral characteristics of which are shown in Figure 2. Image acquisition was by way of a monochrome CCD camera and Kinetic Imaging (Liverpool UK) software. Subsequent analysis was carried out using Scion Image (Scion Corporation, MD USA). Scion Image was used to subtract those images, and further detailed information was obtained on the localization of macular pigment. The intensity of the background was normalized in both images before subtraction.

Figure 2

Spectra of narrow band pass filter 460 and 530 nm used in this study.


In the 11 macular specimens the extent of the visible macular pigment in both measurements was very similar and comparable. Its distribution varied between 200 and 900 μm in the evaluation of consecutive 100 μm vibratome sections and between 180 and 700 μm in the analysis of visible MP in foveal sections (Table 1). In some retinas macular pigment could only be detected in an area of 180–300 μm diameter around the centre of the fovea, whereas in other individuals macula pigment was visible in a diameter between 700 and 900 μm around the centre of the fovea (Figure 3a and b). The results of the foveal section measurements were always slightly smaller than the analysis of the consecutive vibratome sections, which might be the result of the sections sometimes being more than 100 μm thick. In addition all sections with visible macular pigment were counted independent of the intensity of the MP, therefore sections where the MP do not extend completely through 100 μm were counted, too.

Table 1 Spatial distribution and extension of visible macular pigment in 11 human retinas of different age
Figure 3

Two examples for the divergent spatial distribution of the macular pigment in the human fovea. The foveal section (a) and parafoveal section with 200 μm distance to the centre (b) of specimen 10 Table 1.The MP with an extension about 600 μm is well visible in both photographs. In contrast in the case of MP extension of 300 μm the MP is visible only in the foveal section (c), but not in the parafoveal section in 200 μm distance to the centre (d). (c) and (d) are from specimen no. 5 Table 1. Magnification: × 25.

In this limited group of donors, no relationship between spatial extent of macular pigmentation and age was observed. In particular, there was no consistent decrease in the extent of macular pigment visible with age.

The predominant localization of the macular pigment was in the fibres of Henle and plexiform layers (Figure 3a). Because the fibres have in the outer plexiform layer a horizontal extension into the inner nuclear layer where they synapse onto bipolar cells, in parafoveal sections the macular pigment was also visible in the inner and outer plexiform layers between the cell nuclei of the nuclear layer (Figure 3b). When the extension of macular pigment was less than 400 μm, it was not observed in sections more than 200 μm from the fovea (Figure 3c and d).

When the sections were photographed with blue light (460 nm filter) the macular pigment appears dark because the light is absorbed (Figure 4a). Subsequently when green light is used (530 nm filter) the macular pigment is not visualized (Figure 4b). When the image obtained with green light is subtracted from the image obtained with blue light, the localization of the macular pigment is further enhanced as shown in Figure 4c.

Figure 4

Visualization of the macular pigment in the human fovea by narrow band pass filters. The absorption of blue light by the foveal MP is visible as dark structures using a 460 nm filter (a). With a filter of 530 nm the green light is not absorbed by the MP (b). Owing to digitized subtraction of green from blue light photographs only the MP can be demonstrated as dark structures (c). Presence and distribution of MP is shown with more contrast compared to the colour photographs of the same specimen Figure 3a, b. Magnification: × 25.


To reduce the potentially damaging effects of retinal irradiation, several antioxidative and protective mechanisms exist in different layers of the central retina.4, 5 Beside proteins like gluthathione these protective mechanisms include vitamins A, C, and E and the importance of a reduction in these as potential risk factors for the development of AMD is well supported.3, 6, 7, 8, 9, 10, 11, 12, 13 Carotenoids comprise a large number of different molecules and as a group demonstrate high antioxidative properties. In the retina lutein and zeaxanthin are especially abundant.14, 15, 16, 17, 18, 27 These two compounds have two different antioxidative functions in the macula. First their absorbance spectrum, with specific absorption between 400 and 500 nm, will reduce the amount of incoming blue light and second these molecules have several double bonds and they can act directly as free radical scavengers.20, 21, 26

In the present histological evaluation of macular pigment in the human retina of donors of different ages using a two-dimensional analysis, a variation of the extent of MP between 200 and 900 μm was observed. In comparison with studies in monkeys a much wider and larger variation of MP was observed in human macular specimens.21, 26

The localization of macular pigment within the retina was always consistent with deposition of macular pigment in the fovea in the fibres of Henle, whereas parafoveally the visible macular pigment was located in the inner and outer plexiform layers, where additional axons with macular pigment are located (Figure 4). The results obtained using human retinal sections in this study compare well with those obtained using monkey retinas.21, 26 The main difference between monkey and human macula is the smaller size of the foveal depression in humans. In most samples we noticed some bending of the retina in the fovea because of the thinner architecture of the retina at that location.

These results support the conclusions drawn form clinical studies that both the distribution and density of macular pigment varies between individuals, and that this does not correlate well with the density at the foveola.28, 29, 30 This is important in the light of the current interest in the potential role of macular pigment in reducing the risk of visual loss in AMD and the possible manipulation of risk by dietary supplements.

In the assessment of macular pigment both attributes must be taken into account. It is not known if either or both the density and/or distribution is important in protection and can be manipulated by diet. It is also important in certain methods of measuring macular pigment in which the density is assessed by comparing the absorption of light at the fovea and at an eccentric site. If the reference site is not eccentric enough to be outside the region containing macular pigment a false impression may be made. For example, if supplementation causes the density of pigment to rise in the para-foveal region but not at the foveola, measurements may indicate a false impression of loss of macular pigment.

Thus any technique must take into account the density of macular pigment at the fovea and its distribution. In addition this individual specific localization and concentration of macular pigment should be recognized in further studies on the importance of individual variation in the amount of macular pigment as a risk factor for AMD.


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D Pauleikhoff and P Hermans are supported by the German Research Foundation (DFG Pa 357/5-2); FJGM van Kuijk is supported by the Foundation Fighting Blindness and by Research to Prevent Blindness; AC Bird is supported by the Foundation Fighting Blindness.

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Correspondence to D Pauleikhoff.

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Conflict of interest: These authors state no conflict of interest

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Trieschmann, M., van Kuijk, F., Alexander, R. et al. Macular pigment in the human retina: histological evaluation of localization and distribution. Eye 22, 132–137 (2008).

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  • macular pigment
  • oxidative damage
  • age-related macular degeneration
  • lutein
  • zeaxanthin

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