The relative expression ratio of KIF20A mRNA in pancreatic cancer tissues (Pancreatic Ca) and in various normal tissues based on a cDNA microarray analysis. The KIF20A gene was overexpressed in all six pancreatic cancer tissues investigated, but barely expressed in many normal tissues, except for in the testis and thymus. The ratio of relative expression levels of KIF20A mRNA in six pancreatic cancer tissues to that of disease-free counterparts was calculated. For normal tissues, the average expression level of KIF20A mRNA in all tissues was assigned to be 1.0, and the relative expression level of KIF20A mRNA in each tissue was calculated. For pancreatic cancer, the expression levels of KIF20A mRNA in adjacent normal pancreatic tissues were assigned to be 1.0, and the relative expression levels of KIF20A mRNA in pancreatic cancers were calculated using specimens obtained from six patients with pancreatic cancer.