Figure 5 | British Journal of Cancer

Figure 5

From: Pre-clinical evaluation of cyclin-dependent kinase 2 and 1 inhibition in anti-estrogen-sensitive and resistant breast cancer cells

Figure 5

Effect of tamoxifen, NU2058 or NU6102 on phosphorylated pRb levels and cell cycle distribution in synchronously growing MCF7 cell lines. (A) Phosphorylated and total pRb in lysates of exponentially growing cells (+), cells serum starved for 24 h (−) or serum starved for 24 h and released into full medium in the presence of 0.1% (v/v) DMSO, 2 or 8 μ M tamoxifen, 25 or 75 μ M NU2058, 5 or 15 μ M NU6102 for 8 and 24 h after release. (B) Flow cytometric analysis of cells treated as described in A and harvested at 24 h after serum release, expressed as the mean G1 (solid bar), S (open bar), G2/M (grey bar) cell cycle fractions from three independent experiments.

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