Upper panels. Immnunohistochemical stain for p65 in prostate cancer tissues. (A) Prostate cancer staining with the antibody to NF-κB/p65 (C-20, sc-372, Santa Cruz) dilution 1 : 180, magnification × 400. (B) Immunoreactivity was blocked by preabsortion of the primary antibody with the antigen peptide (sc-372 P, Santa Cruz), magnification × 400. Lower panels. Immunofluorescence stain for p65 prostate cancer cells (PC-3). (C) Inmunofluorescence staining with the antibody to NF-κB p65 (C-20, Santa Cruz, dilution 1 : 180) in nonstimulated human prostate cancer cells (PC-3) shows only cytoplasmic staining. (D) PC-3 cells stimulated with TNF-α 100 ng ml−1 for 10 min exhibit nuclear staining.