Constitutive nuclear factor-kappa B mRNA, protein overexpression and enhanced DNA-binding activity in thymidylate synthase inhibitor-resistant tumour cells

In this study, the gene copy number, mRNA and protein expression levels and nuclear DNA-binding activity of nuclear factor kappa B (NF-κB) were compared in a panel of five pairs of thymidylate synthase (TS) inhibitor-resistant and wild-type parent cancer cell lines. High constitutive NF-κB DNA-binding activity was detected in all chemoresistant cell lines. The upregulated NF-κB activity was composed of NF-κB subunits p50 and p65. Four out of five resistant cell lines constitutively overexpressed NF-κB p50 and p65 mRNA and protein. One resistant cell line with the highest NF-κB DNA-binding activity showed normal p50 and p65 protein expression. No NF-κB gene amplification was detected in resistant cell lines. Transient exposure of wild-type RKOWT and H630WT cells to 5-FU induced NF-κB DNA-binding activity but had no effect on NF-κB protein expression in these cells. Our results indicate that high constitutive NF-κB activity caused by its gene overexpression is an intrinsic character of TS inhibitor-resistant cells. NF-κB can antagonise anticancer drug-induced apoptosis. High NF-κB expression and nuclear activity in TS inhibitor-resistant cancer cells may play an important role in the chemoresistance.

The production of the pyrimidine nucleotide TMP from deoxyuridine monophosphate is a critical step in the de novo pathway of DNA synthesis. Since this reaction is catalysed by thymidylate synthase (TS, EC 2.1.1.45) using folate cosubstrate 5, 10-methylene tetrahydrofolate and is the only source of de novo cellular thymidylate, it makes TS an attractive molecular target for cytotoxic drugs (Jackman and Calvert, 1995;Rustum et al, 1997). TS inhibitors (5-fluorourocil (5-FU) and tomudex (TDX)) block TS activity and have been effectively used in the treatment of human solid tumours such as colorectal, breast and lung cancers (Burris et al, 1994;Cunningham et al, 1994;Smith et al, 2002). As with other anticancer drugs, acquired or inherent resistance to these drugs is still one of the main barriers for their clinical use. Apart from high levels of TS protein expression , the other molecular mechanisms of TS-inhibitor-resistance are still largely unknown.
Nuclear factor-kappaB (NF-kB) is a transcription factor that is composed of five subunits (p50/p105, p52/p100, p65 (RelA), RelB and c-Rel). NF-kB can bind to DNA target sites (kB sites) and influence downstream gene expression (Perkins, 2000). The inhibitor of kB (IkB) is a natural inhibitor of NF-kB. In most normal cells, IkB binds to NF-kB and retains NF-kB in the cytoplasm as an inactive complex. Many chemical and biological stimuli can induce IkB phosphorylation, ubiquitinisation and subsequent degradation. NF-kB will be released from the NF-kB-IkB complex and translocated into the nucleus. The liberated NF-kB then binds to the promoter region of the relevant downstream genes and triggers a series of transcriptional events (Karin, 1999).
Some anticancer drugs, cytokines and radiation can induce NF-kB activity in cancer cells (Baldwin Jr, 1996;Pahl, 1999). NF-kB antagonises the cytotoxicity of some anticancer drugs by inducing the expression of anti-apoptotic genes (e.g. c-IAPs, IXAP, A1/Bfl-1 and IEX-IL) (Wang et al, 1998(Wang et al, , 1999bWu et al, 1998). Human cancer cells with induced NF-kB nuclear activity have demonstrated resistance to apoptosis induced by chemotherapy or radiotherapy (Barkett and Gilmore, 1999;Rayet and Gelinas, 1999;Baldwin Jr, 2001;Yamamoto and Gaynor, 2001). Transfection of mutant super-repressor IkBa into tumour cell lines can inhibit NF-kB activity and enhance the cytotoxicity of anticancer drugs in vitro (Wang et al, 1996) and in vivo (Wang et al, 1999a;Cusack et al, 2001).
In this study, nuclear NF-kB activity, the protein and mRNA expression levels of NF-kB in five pairs of TS inhibitor-resistant and sensitive cell lines were tested. The results demonstrate that constitutive high nuclear NF-kB activity and overexpression of NF-kB protein and mRNA are intrinsic characteristics of TS inhibitorresistant cell lines.

Cell culture
Three TDX-resistant cell lines (colorectal tumour: H630 TDX and RKO TDX ; lymphoblastoid: W1L2 TDX ), two 5-FU-resistant colorectal cancer cell lines (H630 5-FU and R10 5-FU ) and the relevant parent cell lines were chosen for this study (The chemoresistant cell lines were kindly provided by Professor PG Johnston, Department of Oncology, The Queen's University of Belfast). The characteristics and culturing conditions have been described previously (Boonsong et al, 2000;Wang et al, 2001). To rule out induction of NF-kB by the maintenance dose of 5-FU/TDX, all drug-resistant cells were cultured in drug-free RPMI 1640 medium supplemented with 10% fetal calf serum, 50 U ml À1 penicillin, 50 mg ml À1 streptomycin for 10 days before harvesting by trypsinisation for further analysis.

Western blot analysis
The cytoplasmic protein (40 mg lane À1 ) was electrophoresed through a 10% SDS -PAGE and transferred to a PVDF membrane (Millipore, UK). The blots were stained with rabbit polyclonal primary (p50, 1 : 500; p65 and IkBa, 1 : 200; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and HRP-conjugated donkey antirabbit secondary (1 : 5 000; Amersham Pharmacia Biotech, NJ, USA) antibodies, respectively, for 1 h at RT. The loading quantity of protein was verified by staining the same membranes with monoclonal anti-a-tubulin or antivinculin antibody (Sigma-Aldrich, Dorset, UK; anti-a-tubulin: 1 : 2000; antivinculin: 1 : 1000) and HRP-conjugated sheep anti-mouse secondary antibody (1 : 5000; Amersham Pharmacia Biotech, NJ, USA). The signal was detected using an ECL Western blotting detection kit (Amersham Pharmacia Biotech, NJ, USA). The intensity of the bands was scanned and analysed using Molecular Analyst software (Bio-Rad Laboratories, CA, USA). This programme was also used to analyse the band intensity of Northern and Southern blots.

Northern blot analysis
Total RNA was extracted using the previously described method (Wang et al, 2001). Briefly, cells (B5 Â 10 6 ) were digested in denaturing buffer and protein was extracted twice with phenol (pH 4.3) : chloroform (1 : 0.3). Total RNA in the aqueous phase was precipitated with an equal volume of isopropanol and then washed with 70% ethanol. Total RNA (20 mg) from drug-sensitive and resistant cells was electrophoresed through a 1% agarose gel containing 1.9% formaldehyde. After transfer to a Hybond-N + nylon membrane (Amersham), the blotted RNA was immobilised by exposing under 250 nm UV light for 3 min. Membranes were hybridised with [ 32 P] dATP random labelled probes at 681C overnight. The signals were detected by exposing the membranes to a FUJI Phosphor Plate overnight and scanned using a FUJI FILM-3000 Phosphor-Imager. Blots were stripped and rehybridised with different probes. NF-kB p50 and p65 subunits were subjected to Northern blotting analysis and GAPDH gene was used as an internal control. The probes were reverse transcribed and amplified from 1 mg RKO TDX total RNA using a one-step RT -PCR kit (Promega, CA, USA). The sequences of primers are as follows: NF-kB p50 (601 bp): forward 5 0 -CTGGTGATCGTGGAACAGCC-3 0 ; reverse 5 0 -CAGAGCCTGCTGTCTTGTCC-3 0 ; NF-kB p65 (659 bp): The sequence of each amplified probe was confirmed by direct automated DNA sequencing.

Southern blot analysis
The DNA was extracted using Nucleon DNA purification kit (Nucleonbiosciences, Glasgow, UK) following the supplier's instruction. A total of 20 mg of DNA from each cell line was digested with MvaII (p50) or SacI (p65) for 3 h at 371C. The digested DNA fragments were separated through a 0.6% agarose gel. After denaturing in 0.1 N NaOH, the DNA was blotted onto a nylon membrane by overnight capillary transfer and fixed under 250 nm UV light for 3 min. The probes, hybridisation and image analysis conditions were the same as those for Northern blotting analysis. The same membrane was stripped and hybridised to different probes.

RESULTS
Higher constitutive nuclear NF-jB activity in TS inhibitorresistant cells NF-kB nuclear activity was tested in five pairs of TS inhibitors (5-FU and TDX)-sensitive and -resistant cell lines. All resistant cell lines were cultured in drug-free medium for 10 days before EMSA analysis. There was substantial NF-kB activity in the chemosensitive parent cell lines, but the TS inhibitor-resistant cell lines demonstrated much higher nuclear NF-kB activity ( Figure 1A). The ratio of NF-kB nuclear activity in paired resistant and wildtype cell lines ranged from 1.8 to 3.5 ( Figure 1B). To verify the bands, 5 mg of nuclear extract mixture from resistant cell lines was preincubated with 20 times higher concentration of unlabelled wild-type or mutant NF-kB probe, before EMSA. The retarded bands were removed by unlabelled wild-type NF-kB probe but not the mutant one ( Figure 1C, lanes 4 and 5). Supershift analysis showed that NF-kB p50 and p65 were involved in the constitutive NF-kB activity in the resistant cells ( Figure 1C, lanes 2 and 3). No supershift band was observed by using antibodies against NF-kB subunits p52, c-Rel and RelB (data not shown). Anti-VEGF antibody was used as a negative control for the supershift assay. To test if the high NF-kB nuclear activity in drug-resistant cells was caused by IkBa degradation, IkBa protein levels in drugsensitive and -resistant cells were assessed by Western blotting analysis. It seems that IkBa degradation was not responsible for the high constitutive NF-kB nuclear activity in resistant cells because high and comparable IkBa protein levels were detected in both resistant and relevant parent cell lines ( Figure 1D). Therefore, we tested protein and mRNA levels of NF-kB subunits p50 and p65 in drug-resistant cell lines. As shown in Figure 2, p50 and p65 protein and mRNA were 1.5 to three-fold constitutively overexpressed in all TS inhibitor-resistant cell lines except for H630 TDX cells in which both proteins were only slightly upregulated. These results indicate that although high NF-kB nuclear DNA-binding activity is mainly caused by enhanced nuclear translocation and DNA-binding affinity, transcriptional and translational deregulation may also play a role in the maintenance of high constitutive NF-kB nuclear activity in TS inhibitor-resistant cells. We have demonstrated before that the constitutive TS gene overexpression in these cell lines was accompanied by gene amplification (Wang et al, 2001). We therefore tested if the high constitutive NF-kB activity in these cell lines was also caused by gene amplification. Gene copy number of NF-kB p50 and p65 was detected by Southern blotting analysis. As demonstrated in the bottom panel of Figure 2A, compared with relevant parent cell lines, no p50 and p65 gene amplification was detected in the drug-resistant cell lines. The change of NF-kB DNA-binding activity in most drug-resistant cell lines was parallel to the increased p50 and p65 protein expression levels. In contrast, the DNA-binding activity in H630 TDX cells was most highly upregulated but the expression of p50 and p65 proteins in this cell line was maintained at similar levels to those of the parent cell line ( Figure 2B). Transient exposure of cancer cells to 5-FU induced NF-jB nuclear DNA-binding activity but not the expression of NF-jB p50 and p65 protein Anticancer drugs exposure can induce NF-kB activity in cancer cell lines (Yamamoto and Gaynor, 2001). To test the effect of 5-FU on NF-kB DNA-binding activity and protein expression levels, colorectal cancer cell lines H630 WT and RKO WT were exposed to 5-FU at different concentrations and time lengths. Figure 3A demonstrates that NF-kB DNA-binding activity was induced by 5-FU in both a time-and concentration-dependent manner in both H630 WT and RKO WT cell lines, whereas exposure of these cells to 30 mM 5-FU up to 24 h had no effect on the expression levels of both NF-kB p50 and p65 proteins in the tested cell lines.
DISCUSSION 5-FU and TDX have been successfully used in human solid cancer chemotherapy. Both drugs inhibit TS, the enzyme converting 2 0deoxyuridine to thymidylate by reductive methylation. 5-FU can also induce metabolic disruption and cell death by incorporating into DNA and RNA. After chemotherapy, some cancer cells show acquired chemoresistance to TS inhibitors. The drug-resistant cells usually demonstrate TS protein and mRNA overexpression and TS gene amplification (Wang et al, 2001). Alternative molecular mechanisms of resistance to TS inhibitors have not been fully elucidated. In this study, we demonstrated high NF-kB DNAbinding activity in a panel of five TS inhibitor-resistant cell lines. NF-kB in resistant cell lines was composed of p65/p50 and p50/p50 dimers, which are the most common NF-kB dimers and play a major antiapoptotic role in anticancer drug-treated mammalian cells (Wang et al, 1996(Wang et al, , 1999aCusack et al, 2000). Anticancer drugs exposure can induce NF-kB activity in cancer cell lines (Yamamoto and Gaynor, 2001). The half-life of NF-kB is less than 30 min and maintenance of its activity requires ongoing protein synthesis and continuous stimuli (Hohmann et al, 1991). To rule out the possibility that the enhanced NF-kB DNA-binding activity in resistant cell lines was also induced by the maintaining dose of 5-FU or TDX, the resistant cell lines were cultured in drug-free medium for 10 days before EMSA. The cells demonstrated comparable NF-kB DNA-binding activity whether or not they were cultured in the medium with or without maintenance concentrations of TS inhibitors (data not shown). In comparison with relevant parent cell lines, drug-resistant cells demonstrated higher NF-kB DNA-binding activity. Thus, the high NF-kB activity in drug-resistant cells is a constitutive and intrinsic feature of the resistant cell lines. NF-kB is a transcription factor that antagonises apoptosis induced by some anticancer drugs (Yamamoto and Gaynor, 2001). Cancer cells with high induced NF-kB activity show a high expression of antiapoptotic gene products such as c-IAPs, IXAP, A1/Bfl-1 and IEX-IL (Wang et al, 1998). In our study, high NF-KB activity in TS-inhibitor resistant cell lines may at least partially lead to their chemoresistance. Figure 3A demonstrates that NF-kB activity could be induced by transient exposure of colorectal cancer cell lines to 5-FU. In combination with 5-FU, disulfiram, an NF-kB inhibitor, strongly enhances the cytotoxicity of 5-FU and totally reverses 5-FU resistance in two 5-FU-resistant colorectal cancer cell lines in vitro (Wang et al, 2002). Thus, targeting NF-kB may be a new measure to overcome TS inhibitor resistance.
High transcription factor activity in cancer cells is frequently induced by gene amplification and/or overexpression (Adams and Kaelin, 1996). In this study, we demonstrated that overexpression of NF-kB p50 and p65 protein and mRNA was detected in four out of five drug-resistant cell lines. The overexpressed NF-kB protein levels in these cell lines were in parallel with those of enhanced NF-kB nuclear binding activity. Thus, the constitutive gene overexpression may be the main cause of the high NF-kB nuclear activity in these cell lines. Although NF-kB DNA-binding activity in drug-sensitive cancer cell lines could be induced by transient exposure to 5-FU, no alteration of NF-kB protein levels was observed in these cell lines ( Figure 3A transcriptional, translational, nuclear transporting and DNAbinding levels because no gene amplification was detected by Southern blotting. We have tested mRNA expression patterns in the same panel of cell lines using Atlas nylon membrane cDNA array system before and could not identify the mRNA overexpression of NF-kB p65 that was spotted on the array membrane (Wang et al, 2001). This discrepancy may be caused by the sensitivity of the array system, especially for detecting the expression of low copy number genes.
Compared to other cell lines, the NF-kB DNA-binding activity and protein expression patterns in H630 TDX cells are intriguing. NF-kB nuclear DNA-binding activity in H630 TDX cells was highly upregulated. The NF-kB proteins p50 and p65 in this cell line were similar to those in the parent cells. Apart from gene overexpression, high NF-kB nuclear activity can be caused by enhanced nuclear translocation and DNA-binding affinity (Rayet and Gelinas, 1999). It has recently been reported that NF-kB protein phosphorylation also plays an important role in the regulation of NF-kB DNA-binding and transcriptional activity (Ghosh and Karin, 2002). Our results indicate that high constitutive NF-kB activity in TS inhibitor-resistant cell lines may be caused by different molecular mechanisms.
IkB degradation is thought to be a main reason for induction and maintenance of high nuclear NF-kB activity (Chiao et al, 1994;Karin, 1999). IkBa is the predominant member of the IkB family. IkBa degradation was involved in 5-FU-induced NF-kB activation in 5-FU-sensitive cell lines (Wang et al, 2002). However, in this study, high NF-kB activity in TS inhibitor-resistant cells was not conferred by degradation of IkBa. High levels of IkBa were detected in all drug-resistant cancer cell lines that demonstrated high nuclear NF-kB activity ( Figure 1D). It was reported that constitutive nuclear NF-kB activity in two chemoresistant Figure 3 5-FU induced NF-kB DNA-binding activity but had no effect on the protein expression of NF-kB p50 and p65 in H630 WT and RKO WT cells. After exposure of cancer cells to 5-FU at different concentrations and time lengths, NF-kB DNA-binding activity and NF-kB p50 and p65 protein expression levels were tested by EMSA (A) and Western blot (B), respectively. Oct-1 and vinculin were used as loading control for EMSA and Western blotting, respectively.
lymphoma cell lines was caused by aberrant activation of IKK's and overexpression of defective IkBa (Krappmann et al, 1999). The role of IkBa in NF-kB signalling pathways in TS inhibitor-resistant cell lines remains to be elucidated.