Phosphorothioate oligonucleotides, suramin and heparin inhibit DNA-dependent protein kinase activity

Phosphorothioate oligonucleotides and suramin bind to heparin binding proteins including DNA polymerases, and inhibit their functions. In the present study, we report inhibition of DNA-dependent protein kinase activity by phosphorothioate oligonucleotides, suramin and heparin. Inhibitory effect of phosphorothioate oligonucleotides on DNA-dependent protein kinase activity was increased with length and reached a plateau at 36-mer. The base composition of phosphorothioate oligonucleotides did not affect the inhibitory effect. The inhibitory effect by phosphorothioate oligodeoxycytidine 36-mer can be about 200-fold greater than that by the phosphodiester oligodeoxycytidine 36-mer. The inhibitory effect was also observed with purified DNA-dependent protein kinase, which suggests direct interaction between DNA-dependent protein kinase and phosphorothioate oligonucleotides. DNA-dependent protein kinase will have different binding positions for double-stranded DNA and phosphorothioate oligodeoxycytidine 36-mer because they were not competitive in DNA-dependent protein kinase activation. Suramin and heparin inhibited DNA-dependent protein kinase activity with IC50 of 1.7 μM and 0.27 μg ml−1 respectively. DNA-dependent protein kinase activities and DNA double-stranded breaks repair in cultured cells were significantly suppressed by the treatment with suramin in vivo. Our present observations suggest that suramin may possibly result in sensitisation of cells to ionising radiation by inactivation of DNA-dependent protein kinase and the impairment of double-stranded breaks repair. British Journal of Cancer (2002) 86, 1143–1149. DOI: 10.1038/sj/bjc/6600191 www.bjcancer.com © 2002 Cancer Research UK

Suramin has been used as an anti-cancer agent and an anti-HIV agent (Stein, 1993). The structure of suramin is shown in Figure 1. Potential mechanisms of the anti-tumour effect are inhibition of heparin binding growth factors. Suramin binds to PDGF, basic fibroblast growth factor (bFGF) and other growth factors, and it prevents the binding of growth factors to their corresponding receptors (Hosang, 1985;Coffey et al, 1987;Stein, 1993). Potential mechanisms of the anti-HIV effect are inactivation of reverse transcriptase and DNA polymerases (Mitsuya et al, 1984;Spigelman et al, 1987;Stein, 1993). Suramin is also an inhibitor of Protein kinase C and DNA topoisomerase II (Mahoney et al, 1990;Bojanowski et al, 1992).
Thus both S-oligo and suramin act as heparin mimetics and they inhibit HIV-1 integration into host DNA Benimetskaya et al, 1995). In the present study, we investigated the effects of S-oligo, suramin and heparin on activity of DNAdependent protein kinase (DNA-PK) because DNA-PK is thought to be a heparin binding protein (Lees-Miller and Anderson, 1991) and because it is involved in the retroviral integration into host DNA (Daniel et al, 1999). We demonstrate that S-oligo, suramin and heparin inhibit DNA-PK activity. DNA-dependent protein kinase activities and DNA-double stranded breaks (DSBs) repair of cultured cells were significantly suppressed by the treatment with suramin. Our present observations suggest that suramin may possibly result in sensitisation of cells to ionising radiation by inactivation of DNA-PK and the impairment of DSBs repair.

Cell lines
LM217 is an SV-40 transformed human fibroblast cell line derived from HS27 (Murnane, 1986), and it was obtained from Dr JP Murnane. A human glioblastoma cell line T98G, and a human acute lymphoblastic leukaemia cell line MOLT-4 were obtained from the ATCC (Rockville, MD, USA).

Digestion of S-dC 36
For digestion of S-dC 36 , it was incubated in 2N HCl at 958C for 1 h. After incubation, pH of the solution was adjusted to pH 7.2 using NaOH. The effect of the HCl-treatment was confirmed by polyacrylamide gel electrophoresis and no obvious band was observed.

DNA-PK activity measurement
DNA-dependent protein kinase activity was assayed as previously described using a synthetic peptide (EPPLSQEAFADLWKK) (Hosoi et al, 1998b). Whole cell extracts were prepared as described previously (Hosoi et al, 1998a). The cell extracts were incubated in 20 ml of kinase buffer (20 mM HEPES-NaOH, pH 7.2; 100 mM KCl; 5 mM MgCl 2 ; 1 mM DTT; 0.5 mM NaF; 0.5 mM b-glycerophosphate; 0.2 mM ATP; 10 mCi ml 71 [g-32 P]ATP in the presence of 0.5 mg ml 71 Poly(dG-dC)×Poly(dG-dC) (Amersham Pharmacia Biotech) and 0.5 mg ml 71 substrate peptide) at 378C for 15 min. The final protein concentration in the reaction buffer was 0.15 mg ml 71 . In DNA-PK activity measurement using the purified DNA-PK solution, 0.25 ml of the solution was used in each reaction. The reactions were stopped by the addition of 20 ml of 30% acetic acid and spotted onto P81 paper disks (Whatman International Ltd., Maidstone, UK). The disks were washed four times in 15% acetic acid. Radioactivity in the paper disks was measured in a liquid scintillation counter. The DNA-PK activity is defined as the amount of P transferred from ATP to the synthetic peptide in the reaction. The specific activities of the cell extracts from LM217 cells, those from T98G cells and the purified DNA-PK solution were 19.0+2.7, 72.31+9.48 and 2316.0+128.9 pmol mg 71 protein respectively.

Preparation of plug and irradiation
Cells in dishes were irradiated on ice using an X-ray machine SHIMADZU HF350C (Shimadzu Corporation, Kyoto, Japan) at 200 kV, 20 mA with a 0.5 mm Cu and 1.0 mm Al filter at a dose rate of 1.5 Gy min 71 , and then incubated in a CO 2 incubator at 378C for repair. At each paoin, cells were trypsinised and washed two times with cold PBS, and the resulting cell pellet was embedded in 0.75% agarose (Low Melt Preparative Grade Agarose, Bio-Rad Laboratories). These agarose sample plugs were immersed in ice-cold lysis solution (0.5 M EDTA; 0.01 M Tris; 2% Sarcosyl; 0.2 mg ml 71 proteinase K) for 1 h and then incubated at 508C for 48 h. After lysis, sample plugs were washed for 1 h at room temperature in a buffer containing 10 mM Tris (pH 8.0) and 0.1 M EDTA and then treated for 1 h at 378C in the same buffer with 0.1 mg ml 71 RNase A (Wang et al, 2001 Figure 1 Structure of suramin. The hexasodium salt is shown. The molecular weight of suramin is 1429. Inhibition of DNA-PK activity by S-oligos and suramin Y Hosoi et al (0 h), cells were embedded in agarose and irradiated in ice-cold PBS followed by an immediate lysis as described (Okayasu et al, 1998).
The voltage applied was 6.0 V/cm with a 60-s pulse time for the first 9 h followed by 120-s pulse time for the last 15 h (total run time 24 h) (Okayasu et al, 1998). After electrophoresis, the gels were stained with ethidium bromide (1.0 mg ml 71 ), detained in deionised distilled water (Longo et al, 1997). The images were acquired under UV light using Printgraph AE-6910CX and Image-Saver AE-6905C (ATTO Corporation, Tokyo, Japan) and analysed by NIH image. Inhibition of DNA-PK activity by S-oligos and suramin Y Hosoi et al sponds to FDL without irradiation, was subtracted from each point.

RESULTS
Inhibition of DNA-PK activity was dependent on the chain length of S-dCn DNA-dependent protein kinase activity was measured in the presence of S-dCn with different chain lengths. DNA-dependent protein kinase activity was inhibited by S-dCn depending on the concentration of S-dCn and the number of phosphorothioate linkages (Figure 2A). Inhibitory concentration 50 (IC 50 ) of S-dCn is shown in Figure 3. The IC 50 value decreased from 975 nM to 13 nM with the increased chain length from 12 to 36. The IC 50 value reached a plateau with the 36-mer. To confirm whether this inhibitory effect was specific for LM217, the effect of S-dC 36 on DNA-PK activity was investigated using whole cell extract derived from T98G. The IC 50 in a T98G cell extract was almost the same as that in a LM217 cell extract ( Figure 2B).

Inhibition of DNA-PK activity was independent of the base composition
Next, effect of base composition on inhibition of DNA-PK activity was investigated. Effect of S-dC 36 , S-dG 36 , S-dA 36 , or S-dT 36 on DNA-PK activity is shown in Figure 2C. Inhibition of DNA-PK activity was not affected by the base composition of phosphorothioate 36-mer oligonucleotides.

Direct interaction between oligonucleotides and DNA-PK, and comparison between the effect of phosphodiester oligonucleotides on DNA-PK and that of phosphorothioate oligonucleotides
In order to confirm whether inhibition of DNA-PK activity by Soligos is mediated by direct interaction with DNA-PK, purified DNA-PK was used. S-dC 36 inhibited purified DNA-PK activity with the same IC 50 as cell extracts, which is suggesting direct interaction between S-dC 36 and DNA-PK ( Figure 2D). Next, the effect of dC 36 on DNA-PK was compared with the effect of S-dC 36 . DNA-dependent protein kinase activity was inhibited by dC 36 ( Figure 2C). However, dC 36 showed much less inhibition than S-dC 36 . The IC 50 of dC 36 was about 200 times higher than that of S-dC 36 . Similar inhibition was observed with purified DNA-PK ( Figure 2D).

Inhibition of DNA-PK activity by S-dC 36 was not due to chemical contaminants
The IC 50 of S-dCn was not linearly proportional to the chain length (Figure 3), which suggests that the inhibition was not due to contaminants in the purified phosphorothioate oligonucleotides.
To confirm this, S-dC 36 was digested with HCl treatment at 958C for 1 h and the effect of digested S-dC 36 on DNA-PK was investigated. HCl-treatment decreased the IC 50 value, which is indicating that the inhibition was not due to chemical contaminants ( Figure  2E).
S-dC 36 did not compete with dsDNA for inhibition of DNA-PK activity Single-stranded DNA has been reported to inhibit DNA-PK activity by binding to DNA-PK at a different site from the dsDNA-binding site (Leuther et al, 1999). To gain insight into whether the S-dC 36binding site differs from the dsDNA-binding site, we investigated whether dsDNA competes with S-dC 36 in DNA-PK activation. DNA-dependent protein kinase activation by dsDNA reached its maximal level at a concentration of 0.5 mg ml 71 , remaining unchanged as the concentration increased up to 5 mg ml 71 ( Figure  4). Inhibition of DNA-PK activity by 50 nM S-dC 36 was unaffected by change in dsDNA concentration (Figure 4). This non-competitive inhibition suggests that the S-dC 36 -binding site is distinct from the dsDNA-binding site.

Inhibition of DNA-PK activity by suramin and heparin
Effects of suramin and heparin on DNA-PK activity in cell extracts are shown in Figure 5A,B. Suramin and heparin suppressed DNA-PK activity with IC 50 of 1.7 mM and 0.27 mg ml 71 respectively.  Figure 3 Dose-response curve after treatment with S-dCn. IC 50 was calculated from the data used for Figure 2A. DNA-PK without S-dC 36 DNA-PK with 50 nM S-dC 36 Figure 4 Effect of dsDNA concentration on inhibition of DNA-PK activity by S-dC 36 . Concentrations of dsDNA ranged from 0.05 mg ml 71 to 5 mg ml 71 . Whole cell extract prepared from LM217 was used. Details are the same as shown in Figure 2. Figure 5. Effect of suramin (A) and heparin (B) on DNA-PK activity. Whole cell extract prepared from LM217 was used. Details are the same as shown in Figure 2.

Experimental Therapeutics
Next, we investigated effects of suramin on DNA-PK activity in cultured cells. LM217 cells at growth phase were treated with 1 mM suramin for 20 h. After treatment, cells were washed with PBS three times, total cell extracts were prepared, and DNA-PK activities were measured. DNA-PK activities were significantly suppressed by the treatment with suramin to 80.7+8.7% of the control value (P=0.045). The treatment with 1 mM suramin for 24 h did not affect the plating efficiency of LM217 cells (data not shown).

Inhibition of DNA repair by suramin
Because treatment with 1 mM suramin in vivo suppressed DNA-PK activity to 80.7% of the control value, we investigated the effect of suramin on DNA-repair after irradiation. After 20 h treatment with 1 mM suramin, LM217 cells were irradiated with 50 Gy, and DSBs repair was analysed by PFGE. The FDL is reported to be proportional to the ratio of fragmented double-stranded DNA (Okayasu et al, 1998). Although the initial DSBs (0 h) with 50 Gy irradiation was statistically identical for suramin-treated and untreated cells, the significant inhibition of DSBs repair was observed in samples treated with suramin ( Figure 6).

DISCUSSION
S-oligos bind sequence-independently to a variety of proteins and inhibit their functions (Stein, 1995). S-oligos act as heparin mimetics and they bind to heparin binding proteins such as bFGF (Benimetskaya et al, 1995). Furthermore, S-oligos inhibit HIV-1 DNA integration (Matsukura et al, 1987;Agrawal et al, 1988). In these respects, S-oligos resemble such polyanions as suramin and pentosan polysulphate (Stein, 1995). Suramin and pentosan polysulphate also bind to heparin binding proteins and inhibit HIV-1 DNA integration (Mitsuya et al, 1984;Zugmaier et al, 1992;Stein, 1993). In the present study, we demonstrated that both S-oligos and suramin show the same effects on DNA-PK activity.
Binding of S-oligos to heparin binding proteins is sequenceindependent but is dependent on the chain length of S-oligos. It has been reported that S-oligos with a chain length longer than 15 could bind and inhibit the proteins, and that the inhibitory effect reached a plateau level with a chain length of 28 (Gao et al, 1992;Marshall et al, 1992). In the present study, we reported that the inhibitory effect of S-oligos on DNA-PK activity is sequence-independent and is dependent on the chain length. The inhibitory effect reached a plateau level with a chain length of 36, which is almost the same length as previously reported. Marshall et al (1992) reported that the inhibitory effect of oligonucleotides on HIV-1 reverse transcriptase could be at least 30-fold greater with phosphorodithioate oligonucleotides, which have two sulphur at each site of internucleotide linkages, than with phosphorothioate oligonucleotides. Benimetskaya et al (1995) reported that binding of phosphorothioate oligonucleotides to proteins is independent on P-chirality at the internucleotide linkage sites. These results may suggest that substances having the similar structure to polyanions with sulphur bind heparin-binding proteins and inhibit their functions (Zugmaier et al, 1992;Guvakova et al, 1995). Phosphorothioate oligonucleotides contain a sulphur atom at each phosphorus atom. Each n-mer phosphorothioate oligonucleotide has (n-1)-centres of asymmetry at phosphorous because each linkage can occur as either the Rp-of Sp-diastereomer (Benimetskaya et al, 1995). Phosphorothioate oligonucleotides used in this experiment contained a random mixture of diastereomers that would have a variety of three-dimensional structure. Benimetskaya et al (1995) reported that binding of phosphorothioate oligonucleotides to basic fibroblast growth factor, recombinant soluble CD4, laminin and fibronectin is P-chirality independent. It is unknown what effect the three-dimensional structure may have on the interactions of phosphorothioate oligonucleotides with DNA-PK. Both S-oligos and suramin inhibit the binding of HIV-1 gp120 to CD40 and the enzyme activity of DNA polymerases, RNase H and HIV-1 integrase (Gao et al, 1989(Gao et al, , 1992Stein et al, 1991Stein et al, , 1993Tramontano et al, 1998). All of these function and enzymes are related to HIV-1 infection and integration into host DNA. Recently, DNA-PK was reported to be involved in the retroviral integration into host DNA (Daniel et al, 1999). S-oligos and suramin will be potent anti-HIV-1 agents. In addition, it is interesting that most of the steps of HIV-1 integration into the host DNA are inhibited by S-oligos and suramin.

Experimental Therapeutics
The mechanisms of DNA-PK inactivation by S-oligos and suramin are not known. Hammarsten and Chu (1998) reported that single-stranded DNA (ssDNA) did not inhibit the binding of dsDNA to Ku but it inhibits the binding of dsDNA to DNA-PK. The structure of DNA-PK was revealed by electron crystallography (Chiu et al, 1998;Leuther et al, 1999). Chiu et al (1998) reported that DNA-PKcs protein has an open, cage-like structure, which may allow the insertion of two DNA ends from the two opposing faces of the protein. Leuther et al (1999) reported that structure of DNA-PKcs protein contains an open channel, similar to those seen in other double-stranded DNA-binding proteins, and a cavity which is large enough to accommodate ssDNA. They suggest that ssDNA binds to the enclosed cavity and inhibit DNA-PK activity. These reports suggest that ssDNA binds to DNA-PK at a position different from where dsDNA binds to DNA-PK. In the present study, we reported that inhibition of DNA-PK activity by S-oligos is not competitive with dsDNA, suggesting that the binding position of S-oligos is different from that of dsDNA. Further experiments are required to elucidate this point.
Cells lacking DNA-PK activity due to defects in DNA-PK components show hypersensitivity to ionising radiation because of an important role of DNA-PK in the repair of DNA doublestrand breaks (Jeggo et al, 1995). A phosphatidylinositol 3-kinase (PI3-kinase) inhibitor, wortmannin, inhibits DNA-PK activity, DSBs repair and sensitises cells to ionising radiation (Rosenzweig et al, 1997;Hosoi et al, 1998b). Suramin is reported to be located in the nucleus of cells exposed to suramin (Bojanowski et al, 1992). In the present study, we demonstrated that suramin suppressed DNA-PK activity and DSBs repair in vivo. Our present observations suggest that suramin may possibly result in sensitisation of cells to ionising radiation by inactivation of DNA-PK and the impairment of DSBs repair.