Laminin γ1 chain peptide, C-16 (KAFDITYVRLKF), promotes migration, MMP-9 secretion, and pulmonary metastasis of B16–F10 mouse melanoma cells

Laminin-1, a heterotrimer of α1, β1, and γ1 chains specific to basement membrane, promotes cell adhesion and migration, proteinase secretion and metastases of tumour cells. Several active sites on the α1 chain have been found to promote B16–F10 melanoma lung colonisation and here we have determined whether additional tumour promoting sites exist on the β1 and γ1 chains. Recently, we have identified novel cell adhesive peptides derived from laminin β1 and γ1 chains by systematic screening of synthetic peptides. Nine β1 peptides and seven γ1 peptides active for cell adhesion were tested for their effects on experimental pulmonary metastases of B16–F10 mouse melanoma cells in vivo. The most active adhesive peptide derived from the γ1 chain globular domain, C-16 (KAFDITYVRLKF), significantly enhanced pulmonary metastases of B16–F10 cells, whereas no other peptides showed enhancement. C-16 also stimulated migration of B16–F10 cells in the Boyden chamber assay in vitro. Furthermore, C-16 significantly induced the production of MMP-9 from B16–F10 cells. These results suggest that this specific laminin γ1 chain peptide has a metastasis-promoting activity and might be a new molecular target of anti-cancer treatment. British Journal of Cancer (2002) 86, 1169–1173. DOI: 10.1038/sj/bjc/6600187 www.bjcancer.com © 2002 Cancer Research UK

Several biologically active sites on mouse laminin-1 that affect tumour cells have been previously identified using synthetic peptides (Yamada and Kleinman, 1992). YIGSR on the b1 chain promotes tumour cell adhesion and migration (Graf et al, 1987a,b;Iwamoto et al, 1988) and inhibits experimental pulmonary metastases of mouse melanoma cells and angiogenesis (Iwamoto et al, 1987;Sakamoto et al, 1991). IKVAV on the a1 chain promotes cell adhesion, tumour growth, angiogenesis, collagenase IV activity by tumour cells, and experimental metastases as well as plasmino-gen activator activation (Grant et al, 1989;Tashiro et al, 1989;Kanemoto et al, 1990;Stack et al, 1991;Nomizu et al, 1992).
Recently, we have systematically screened a large set of overlapping synthetic peptides covering the whole mouse laminin-1 for their cell adhesive activities. Five peptides from the G-domain of a1 chain (Nomizu et al, 1995), 12 peptides from the g1 chain (Nomizu et al, 1997), 21 peptides from the short and long arms of a1 chain , and 12 peptides from the b1 chain  were found to have significant cell adhesive activity. In addition, several peptides from the laminin-1 have been identified to be active for angiogenesis Ponce et al, 1999), acinar formation of salivary gland , and neurite outgrowth .
We previously identified several laminin a1 peptides that influence the metastatic activities of B16 -F10 melanoma cells. AG-73 peptide from the a1 G-domain causes liver metastases (Kim et al, 1998), A-13 peptide from the N-terminal globule promotes an increase in pulmonary metastases , and the AG-73 peptide (LQVQLSIR) promotes increased lung colonies and liver metastases. Here we report effects of adhesive peptides from the b1 and g1 chains on experimental pulmonary metastases of B16 -F10 cells in vivo. We have screened other adhesive peptides from b1 and g1 chains for metastatic activity. We found that one of the peptides derived from the short arm of g1 chain, C-16 (KAFDITYVRLKF), significantly enhanced pulmonary metastases of B16 -F10 cells. This peptide also stimulated the migration of B16 -F10 cells in the Boyden chamber assay. In addition, C-16 significantly induced production of MMP-9 by B16 -F10 cells.

Preparation of synthetic peptides
Cell adhesive peptides from the laminin b1 and g1 chain and control peptides were synthesised and purified by high performance liquid chromatography as previously described (Nomizu et al, 1997). The purity and identity of the peptides were confirmed by an analytical HPLC and a Sciex API IIIE triple quadruple ion spray mass spectrometer.

In vivo experimental pulmonary metastasis assay
For the in vivo experimental pulmonary metastasis assay, B16 -F10 cells were detached by 0.02% EDTA in phosphate buffered saline (Versene, Life Technologies, Inc.) and suspended in DMEM (1 000 000 cells ml 71 ). The cell suspension (0.2 ml) was injected into the tail veins of female C57BL6/N mice (9 -12 weeks old). 0.2 mg of cell adhesion peptides (1 mg ml 71 in DMEM) was also intravenously injected within 10 min after the cell injection to exclude cell aggregations by mixture of peptide and cells. The mice were sacrificed 16 days after injection. The lungs were removed, and the number of visible colonies on the surface of the lungs was counted. When many colonies were formed and the number of the colonies could not be counted correctly because of the fusion of the colonies, the number was scored as 500. Five mice were used for each peptide. Duplicate experiments gave similar results.
All animals were maintained in filter top micro isolator cages and provided with sterile water and food under conditions complying with National Institute of Health (NIH) regulations. All manipulations of experimental animals were conducted in a laminar flow hood using strictly controlled procedures adhering to the UKCCCR Guidelines for the Welfare of Animals in Experimental Neoplasia to minimise stress or suffering (UKCCCR, 1998).

In vitro migration assay
Migration of B16 -F10 cells through polycarbonate filters was assayed using 48 well chemotaxis chambers (modified Boyden chamber, Neuro Probe, MD, USA) as described previously . The lower wells of the chamber were loaded with DMEM containing 0.1% bovine serum albumin (BSA, Sigma) (DMEM/BSA) and 100 mg ml 71 of peptide. Versene-detached B16 -F10 cells (50 000 cells per 50 ml in DMEM/BSA) were placed in the upper wells. After a 5 h incubation, cells on the lower surface of the filter were stained with Diff-Quik (Baxter, IL, USA), and counted under a microscope. Each peptide was tested in triplicate and each experiment was repeated at least twice.
Zymography B16 -F10 cells (2 500 000 cells) were plated onto 150 mm dishes with complete media. After 24 h, the media were replaced with serum-free DMEM containing various concentrations of peptides. The conditioned media were collected after a 16 h incubation at 378C in 5% CO 2 and concentrated 50 times by using Centriprep 10 (Amicon). Equal aliquots of the conditioned media (20 ml per lane) were electrophoresed on 10% polyacrylamide gels containing 0.2% gelatin. The gels were washed with 10 mM Tris-HCl (pH 7.4) containing 2.5% Triton-X for 30 min, followed by two changes of 10 mM Tris-HCl (pH 7.4) for 30 min. After an overnight incubation in 50 mM Tris-HCl (pH 8.0) containing 5 mM CaCl 2 and 1 mM ZnCl 2 at room temperature, Coomassie blue was added to visualise the digested gelatin bands.

RESULTS
Effect of cell adhesive laminin b1 and g1 chain peptides on experimental pulmonary metastasis of B16 -F10 mouse melanoma cells Nine peptides active for cell adhesion from the mouse laminin b1 chain  and seven peptides from the g1 chain (Nomizu et al, 1997) were tested for their effects on in vivo experimental pulmonary metastases of B16 -F10 mouse melanoma cells (Table 1). In control experiments (without peptides), the average of 163 metastatic colonies was formed on the surface of the lung within 16 days after B16 -F10 cells were injected into the mouse tail vein (Table 2). Peptide C-16 (KAFDITYVRLKF) from the Nterminal globular domain of the g1 chain significantly enhanced the number of B16 -F10 lung colonies (average of 482 colonies, Table 2 and Figure 1). No other peptides showed stimulation or inhibition of pulmonary metastases of B16 -F10 cells (data not shown). A scrambled peptide of C-16, C-16T (FYAFKKITLVRD), slightly increased the number of B16 -F10 lung colonies (average of 234 colonies with range from 90 to 345), but the difference was not significant (Table 2, Figure 1). These results suggested a sequence-specific enhancement of in vivo pulmonary metastases of B16 -F10 cells by C-16.

In vitro cell migration
In order to study the mechanism of the enhancement of B16 -F10 lung colonisation by C-16, we examined the activities of these 16 cell adhesive peptides for cell migration. Among the 16 cell adhesive peptides, 8 peptides (B-32, 34, 62, 133, 160; C-16, 64, 68) stimulated migration of B16 -F10 cells, with C-16 having the Experimental Therapeutics Cell adhesive peptides were identified by systematic screening of overlapping peptides covering the entire mouse laminin b1  and g1 chains (Nomizu et al, 1997).
Metastasis-promoting sequence in laminin g1 Y Kuratomi et al strongest enhancement activity (Figure 2). The migration -stimulatory activity of C-16 seemed to be comparable to that of peptide A-13 from the a1 chain which previously was shown to be highly active Kuratomi et al, 1999). A control scrambled peptide, C-16T, showed no stimulatory activity of B16 -F10 cell migration.

Gelatin zymography
Because matrix metalloproteinases (MMPs) have been implicated in the metastatic process of tumour cells, we measured MMP production from B16 -F10 cells in the gelatin zymography assays (Figure 3). C-16 enhanced the production of MMP-9 (92 kDa gelatinase) in a dose-dependent manner. With 20 mg ml 71 of C-16, MMP-9 production was increased by approximately eight-fold over that observed with the untreated control. MMP-2 production was not stimulated by C-16. No other cell adhesive peptides from the b1 and g1 chains stimulated the production of MMPs (data not shown).

DISCUSSION
Laminin-1 has been shown to promote the metastatic activity of melanoma cells (Terranova et al, 1982(Terranova et al, , 1984. We previously reported that several peptide sequences including RQVFQVAYIII-KA (A-13), IKVAV, and LQVQLSIR (AG-73) from the laminin a1 chain have such activity (Kanemoto et al, 1990;Kim et al, 1998;Kuratomi et al, 1999). In this report, we identified a new site, C-16 (KAFDITYVRLKF), on the laminin g1 chain which promotes lung colonisation of B16 -F10 cells in an experimental pulmonary metastasis model in mice. C-16 enhanced B16 -F10 colonization about three-fold compared with control. C-16 appears to be the only site on the g1 chain of laminin-1 active for lung colonisation and no site on the b1 chain was found to have this activity. C-16-mediated metastasis promoting activity is comparable to that of A-13 and IKVAV. Since laminin-1 is a highly potent promoter of the malignant Experimental Therapeutics  phenotype, it is not surprising that multiple sites for metastases have been identified. The mechanism by which A-13 and C-16 increase lung colonisation is not known. Cells must adhere, migrate, invade (protease activity), proliferate, and generate blood supply in order to form a growing metastatic lesion. A-13 and C-16 have been found to promote all of these activities except cell proliferation. The role of A-13 and C-16 in angiogenesis in the metastatic lesion has not been tested. We did not examine the vascularity of the lung lesions and it is unlikely that A-13 and C-16 persists in the circulation or tissue for very long after injection. These are small molecules which are cleared very rapidly from the circulation. We speculate that A-13 and C-16 may enhance lung metastases by increasing the invasion activity of the cells.
Laminin-1 stimulates cell migration (Kleinman et al, 1985) and laminin a1 peptides with metastasis-promoting activity also have activity for the migration of B16 -F10 cells . We have shown here that five b1 peptides and three g1 peptides stimulated migration of B16 -F10 cells, with C-16 being most active and comparable to A-13. Thus, laminin-1 has multiple active sites for cell migration, which may function cooperatively.
Matrix metalloproteinases have been thought to be critical in tumour invasion and metastases as well as in angiogenesis (Liotta, 1986;Liotta et al, 1991;Kohn and Liotta, 1995). C-16 showed strong stimulation of MMP-9 production by B16 -F10 cells. The IKVAV peptide from the laminin a1 chain also induces MMP-1 and MMP-9 production from human monocyte/macrophage (Corcoran et al, 1995). AG-73 was also reported to stimulate MMP-9 activity of B16 -F10 cells at the concentration of 50 mg ml 71 (Kim et al, 1998). C-16 is more potent in inducing MMP-9 production than AG-73. C-16 stimulated MMP-9 production nearly eight-fold at a concentration of 2 mg ml 71 . This high activity of C-16 for MMP-9 production as well as for cell migration may explain its potent metastasis-promoting activity.
Recently, expression of the g2 chain of laminin-5, an epithelial cell -specific laminin, was predominantly detected at the invasive front of cancer cells of the colon, pancreas, stomach and oesophagus (Pyke et al, 1995;Soini et al, 1996;Sordat et al, 1998;Koshikawa et al, 1999;Yamamoto et al, 2001). The laminin g2 chain has been suggested to play a key role in the progression of human carcinomas. C-16 is located in the N-terminal globular domain of the laminin g1 chain, and this domain is deleted in the g2 chain. However, it is possible that the deleted N-terminal globular domain is co-expressed with laminin g2 chain by the proteolytic cleavage of the g2 chain and that the cleaved domain induces migration and metastases of tumour cells in human cancers. Indeed, Giannelli et al (1997) have reported that laminin-5 becomes active for promoting cell migration when cleaved by MMP-2. It is possible that these active fragments of laminin-1 promote cell migration when it is degraded similar to laminin-5.
Four novel active sites (IKVAV, AG-73, A-13 and C-16) on mouse laminin-1 have been identified for B16 -F10 cell adhesion, migration, MMP secretion, and metastases in this report and in previous studies (Figure 4) (Kanemoto et al, 1990;Kim et al, 1998;Kuratomi et al, 1999). Interestingly, A-13 and C-16 are in homologous locations on their respective chains at the amino termini, binding to the same receptors and have similar activity (Ponce et al, 2001;and unpublished data). Of the 12 amino acids, three amino acids are identical and five are conserved. Preservation of sequences and activity suggest important functional sites. Degradation of laminin chains may also be important in exposure of these active sites. These active sites might be new molecular targets of anti-cancer treatments which prevent distant metastases of malignant tumours. Furthermore, specific antibodies to these sites and inhibitory peptides might lead to development of therapeutic agents for human malignant tumours.   Figure 4 Novel metastasis-promoting peptides from the mouse laminin 1. AG-73 (Kim et al, 1998) and A-13  were found to promote experimental pulmonary metastases of B16 -F10 cells. Previously, IKVAV was found to promote lung colonisation (Kanemoto et al, 1990). All peptides show stimulation of B16 -F10 cell adhesion and migration activities. C-16, AG-73, and IKVAV stimulate 92-kDa gelatinase (MMP-9) activity of B16 -F10 cells.