Figure 4 | British Journal of Cancer

Figure 4

From: Selection and characterisation of a phage-displayed human antibody (Fab) reactive to the lung resistance-related major vault protein

Figure 4

Western blots of cell lysates of MVP-overexpressing GLC4 ADR cells (A) and the immunoprecipitation products of the anti-MVP mouse Mab LRP-56 (B; left lane) or a control Mab (B; right lane) from a cell lysate of the GLC4 ADR cells. Proteins were loaded, separated and blotted. Blots were incubated with MVP-φ4 (A, left lane and B) or a control Fab (A, right lane) followed by HRP-labelled rabbit-anti-human lambda chain. Colour development was with DAB/chloronaphthol/H2O2. On both blots, MVP-φ4 was reactive with the MVP protein at the expected Mr of 110 000 (arrow).

Back to article page