The development of methods for specific delivery of drugs is an important issue for many cancer therapy approaches. Most of macromolecular drugs are taken into the cell through endocytosis and, being unable to escape from endocytic vesicles, eventually are degraded there, which hinders their therapeutic usefulness. We have developed a method, called photochemical internalization, based on light-induced photochemical reactions, disrupting endocytic vesicles specifically within illuminated sites e.g. tumours. Here we present a new drug delivery concept based on photochemical internalization-principle – photochemical disruption of endocytic vesicles before delivery of macromolecules, leading to an instant endosomal release instead of detrimental stay of the molecules in endocytic vesicles. Previously we have shown that illumination applied after the treatment with macromolecules substantially improved their biological effect both in vitro and in vivo. Here we demonstrate that exposure to light before delivery of protein toxin gelonin improves gelonin effect in vitro much more than light after. However, in vitro transfection with reporter genes delivered by non-viral and adenoviral vectors is increased more than 10- and six-fold, respectively, by both photochemical internalization strategies. The possible cellular mechanisms involved, and the potential of this new method for practical application of photochemical internalization concept in cancer therapy are discussed.
Despite exciting progress in the development of new anticancer agents, the development of appropriate delivery strategies, allowing a therapeutic molecule to reach the target and express a therapeutic function, remains to be one of the most important issues for cancer therapy. Most of anticancer agents (e.g. protein- or nucleic acid-based drugs, some watersoluble chemotherapeutics) are membrane-impermeable big molecules, usually taken into the target cell through the endocytic pathway. Moreover, to target tumour cells, therapeutic molecules are often coupled to different ligands, binding to specific receptors on tumour cells, which also leads to receptor-mediated endocytosis (Dubowchik and Walker, 1999). However, macromolecules usually do not have means for escaping from endocytic vesicles and consequently, degradation inside these vesicles in many cases hinders their therapeutic application (Lloyd, 2000). Thus, it is known that type I ribosome-inactivating protein toxins could have a great potential as anticancer agents if released from the vesicles (Barbieri et al, 1993). Also, inefficient endosomal release of therapeutic nucleic acids, especially if delivered by non-viral vectors, is an important obstacle for cancer gene therapy (Zabner et al, 1995; Luo and Saltzman, 2000). Therefore, the development of endosome-disruptive strategies to liberate therapeutic macromolecules is of great importance for the exploitation of full potential of anticancer drugs.
Recently we have developed a new technology named photochemical internalization (PCI) to improve the cytosolic delivery of various macromolecules that are taken into the cell via endocytosis (Berg et al, 1999). The method is based on the use of photosensitizing compounds, such as aluminium phthalocyanine (AlPcS2a) that localize in endocytic vesicles and upon light exposure induce photochemical damage to the vesicular membranes, releasing intact macromolecules into the cytosol (Berg et al, 1999). PCI, as a light-dependent treatment, is an attractive approach for targeted drug delivery, since in principal only areas exposed to light (e.g. tumours) will be subjected to the drug effects.
We have reported earlier that the PCI principle can be utilized in vitro for transfer of various macromolecules. Thus, the toxicity of proteins, such as the type I ribosome-inactivating toxin gelonin and a tumour-targeted immunotoxin, was considerably increased by photochemical treatment (Berg et al, 1999; Selbo et al, 2000). Likewise, the efficiency of transfection with a reporter transgene was improved 10–20-fold by light (Høgset et al, 2000; Prasmickaite et al, 2000). We have also demonstrated the potential of the PCI technology in vivo in an animal model, where the cytotoxic effect of gelonin, which was completely inactive when used alone, was increased tremendously by photochemical treatment, resulting in a complete regression of subcutaneous tumours in mice (Selbo et al, 2001).
Originally the PCI method was developed to liberate macromolecules, which had already been endocytosed and trapped in endocytic vesicles, i.e. it was based on illumination applied after the treatment with macromolecules (‘light after’ strategy). Although the technology generally works well in this setting, there are several potential disadvantages: (i) the possible enzymatic degradation of the macromolecules in endocytic vesicles before the liberation; (ii) the possible induction of photochemical damage to the macromolecules located close to the photosensitizer at the moment of illumination; (iii) the inconvenience of a sequential treatment in a therapeutic situation. Therefore, the aim of this study was to investigate the possibility of disrupting endocytic vesicles in advance, i.e. before the macromolecules are taken into the cell (‘light before’ strategy). In this report we show that this novel PCI approach can increase the biological effect of both protein toxins and transfecting nucleic acids more or at least as efficiently as our previously described strategy. The implication of the findings for the development of light-directed drug delivery systems for cancer therapy will be discussed.
Materials and methods
Reagents and cell culture
A plasmid pEGFP-N1 (encoding Enhanced Green Fluorescent Protein (EGFP)) was purchased from Clontech Laboratories, Inc. (Palo Alto, CA, USA), AlPcS2a was from Porphyrin Products (Logan, UT, USA). Gelonin, poly-L-lysine hydrobromide (MW 20700) and FITC-dextran (MW 4400) were from Sigma (St. Louis, MO, USA). Fluorescein di-β-D-galactopyranoside was from Molecular Probes (Eugene, OR, USA). Adenovirus AdHCMV-lacZ was kindly provided by Dr FL Graham, McMaster University, Ontario, Canada.
The human malignant melanoma cell line THX was established from a tumour tissue obtained from a patient treated for metastatic malignant melanoma at the Norwegian Radium Hospital. The cells were cultured in RPMI-1640 medium supplemented with 10% foetal calf serum, 100 units ml−1 penicillin, 100 μg ml−1 streptomycin and 2 mM glutamine (all from Bio Whittaker, Walkersville, MD, USA) at 37°C in 5% CO2 atmosphere.
Treatment with different macromolecules
For the treatment with gelonin 25 × 103 cells per well were seeded out into 24-well plates (Nunc, Denmark). For the transfection with pEGFP-N1/polylysine and transduction with AdHCMV-lacZ 50 × 103 THX cells per well were seeded out into 6-well plates. The next day 20 μg ml−1 AlPcS2a were added, and the cells were incubated for 18 h at 37°C. All the procedures after AlPcS2a addition were carried on in subdued light. For the ‘light before’ strategy AlPcS2a was removed, the cells were washed three times and incubated in AlPcS2a-free medium for 4 h. Then the cells were exposed to light (see below) before the treatment with selected macromolecules: either with gelonin (1 μg ml−1 for 18 h), or with the pEGFP-N1/polylysine complex (5 μg ml−1 pEGFP-N1, for different time depending on the experiment), or with AdHCMV-lacZ (at a multiplicity of infection (MOI) 1 for 30 min). For the ‘light after’ strategy the cells were first treated with the selected macromolecules at the same concentrations and for the same time as indicated above, washed, and after addition of fresh culture medium exposed to light. Non-illuminated cells were treated in a similar way except for illumination.
The treated cells were washed once with culture medium, and after addition of fresh medium incubated at 37°C before further analysis. Inhibition of protein synthesis was assayed by [3H]leucine incorporation into proteins 24 h after light exposure as previously described (Llorente et al, 1998). EGFP and β-galactosidase expression was analyzed by flow cytometry (see below) 2 days after illumination.
Illumination was performed from a bench of four light tubes (Philips TLD 18W/79) and a long pass filter with a cut off at 550–600 nm. The light intensity reaching the cells was 13.5 W m−2. Light dose used for transgene delivery induces approximately 50% toxicity (i.e. D50).
pEGFP-N1/polylysine complex (charge ratio 1.7) was prepared by gently mixing plasmid and polylysine solutions prepared separately: 5 μg pEGFP-N1 plasmid was diluted in 75 μl water and 5.3 μg polylysine diluted in 75 μl water. The solutions were mixed and incubated for 30 min at room temperature, diluted with culture medium to 1 ml and added to the cells.
Flow cytometry analysis
For the detection of EGFP, the cells were resuspended in 400 μl of culture medium and analyzed by a FACS-Calibur flow cytometer (Becton Dickinson, San Jose, CA, USA). For each sample 10 000 events were collected. EGFP was measured through a 510–530 nm filter after excitation with an argon laser (15 mW, 488 nm). Dead cells were discriminated from single viable cells by gating on forward scattering vs side scattering. The data were analyzed with CELLQuest Software (Becton Dickinson).
The expression of β-galactosidase was measured as previously described (Nolan et al, 1988). Briefly, the cells were resuspended in 25 μl of culture medium and incubated for 5 min at 37°C. 25 μl of 2 mM fluorescein di-β-D-galactopyranoside were added and incubated for 1 min at 37°C. The suspension was diluted by adding 450 μl ice cold culture medium, and the samples were maintained on ice for 30–60 min before analysis by flow cytometry using the same settings as described above.
THX cells were seeded out into Falcon 3001 dishes (25 × 103 cells per dish) and the next day treated with 20 μg ml−1 AlPcS2a for 18 h, washed and incubated in AlPcS2a-free medium for 4 h. Then the cells were exposed to light for 4 min before a 3 h incubation with 5 mg ml−1 FITC-dextran. Non-illuminated cells were treated in a similar way except for illumination. The intracellular localization of FITC-dextran in unfixed cells was observed with a Zeiss Axioplan fluorescence microscope (Oberkochen, Germany) using an objective with 63 × magnification, a 450–490 nm band pass excitation filter and a 510–540 band pass emission filter. Fluorescence micrographs were recorded by means of a cooled charge-coupled device (CCD) camera (Photometrics Inc., Tucson, AZ, USA).
Effect of photochemical treatment on the intracellular localization of an endocytic marker
First we studied by fluorescence microscopy, whether photochemical treatment applied before the incubation with an endocytic marker (FITC-dextran) affects the intracellular localization of the marker. As can be seen in Figure 1A, cells that were treated with AlPcS2a and FITC-dextran in the absence of light show granular FITC fluorescence, indicating, as expected, localization of FITC-dextran in endocytic vesicles. In contrast, exposure to light before the incubation with FITC-dextran leads to diffuse FITC fluorescence (Figure 1B), suggesting that a substantial fraction of FITC-dextran entered the cytosol instead of accumulating in endocytic vesicles. However, when AlPcS2a-treated cells were exposed to light followed by incubation with FITC-dextran at 4°C, when endocytic uptake is inhibited, no intracellular FITC fluorescence was detected (data not shown), indicating that: (i) endocytosis is important for the uptake of FITC-dextran into photochemically treated cells; (ii) photochemical treatment does not permeabilize cellular membrane so that FITC-dextran could passively diffuse into the cell cytosol.
Effect of photochemical treatment on the cytotoxic effect of gelonin
The protein toxin gelonin is taken into the cell via endocytosis, but is trapped in endocytic vesicles and finally degraded, therefore it is relatively non-toxic to intact cells. However, in cell-free systems gelonin is a very potent inhibitor of protein synthesis, indicating that the inability to escape from endocytic vesicles is the major cause for the low toxicity to intact cells (Stirpe et al, 1980). As can be seen in Figure 2, gelonin had no effect on protein synthesis when applied in the absence of photochemical treatment. However, exposure of AlPcS2a-treated cells to light before the incubation with gelonin considerably increased the cytotoxicity of gelonin. Comparison of the ‘light before’ and the ‘light after’ approaches in THX cells shows that gelonin exhibited much stronger toxicity when light was applied before gelonin-treatment as compared to light after (Figure 2).
Effect of photochemical treatment on gene transfection
The effect of photochemical treatment on delivery of transgenes was studied by measuring the efficiency of transfection with a plasmid, containing a reporter EGFP-gene driven by the cytomegalovirus promoter. The cationic polypeptide poly-L-lysine was used for DNA complexation, and the transfection efficiency was evaluated measuring EGFP expression. As can be seen in Figure 3A, light exposure increased the amount of EGFP-positive cells by >10-fold as compared to non-illuminated cells, irrespectively of whether light was applied before or after the DNA complex.
Photochemical treatment employing either ‘light before’ or ‘light after’ strategy also stimulated transfection mediated by the targeted transfection agent transferrin-poly-L-lysine (data not shown).
Effect of photochemical treatment on adenovirus-mediated gene transduction
In order to study the effect of illumination before gene transduction with viruses (the most used vectors in cancer gene therapy clinical trials), we used the adenovirus AdHCMV-lacZ, containing a β-galactosidase reporter gene controlled by the cytomegalovirus promoter. The cells were pre-incubated with AlPcS2a followed by light exposure before or after a 30 min incubation with AdHCMV-lacZ. As can be seen in Figure 3B, the percentage of β-galactosidase-expressing cells was increased approximately six-fold by both light-treatments, as compared to control cells that were infected with the adenovirus, but not exposed to light. Higher virus doses (i.e. higher MOI) gave higher percentage of transducted cells in both non-illuminated and photochemically treated cell populations (data not shown), however, in general, fold-increase achieved by photochemical treatment is the highest for low virus doses.
Effect of illumination time point
For the potential use of PCI as a method for light-induced delivery in vivo, it was important to define the ‘time window’ for achieving the maximal effect. Thus, THX cells were given a 30 min pulse with the DNA/polylysine complex and were illuminated at different time points before or after the DNA pulse. As can be seen in Figure 4, transfection using the ‘light before’ strategy was most efficient when light was given right before the DNA complex. A 5 h delay reduced the efficiency of transfection by approximately 50%, suggesting that the transfection-enhancing effects induced by light last for up to at least 5 h, albeit decreasing with time. For comparison, using the ‘light after’ strategy, the highest amount of EGFP-positive cells was obtained when light was applied immediately after the DNA complex, indicating that both the DNA-treatment and the light-treatment should be strongly co-ordinated in time, optimally following each other without any delay.
In this study we present a new drug delivery principle – photochemical disruption of endocytic vesicles in specific regions of the body, for improving internalization of later applied macromolecular drugs. This photochemical method offers for cancer therapy a way for obtaining drug effects specifically in tumour cells. The technology can be used with various macromolecules that are taken into the cell by endocytosis, and whose therapeutic activity is normally hampered by the inability to escape from endocytic vesicles. Unlike our earlier described method (Berg et al, 1999; Høgset et al, 2000; Selbo et al, 2000, 2001), where illumination was performed after the treatment with macromolecules (Figure 5A), and unlike most other methods for endosomal liberation, the strategy presented in this paper suggests permeabilization of endocytic vesicles before delivery of macromolecules, avoiding a prolonged and possibly detrimental stay of the molecules in endocytic vesicles. In effect this leads to a very rapid relocalization of the endocytosed macromolecules into the cytosol, and consequently improves their biological effect. In this study we demonstrate that ‘light before’ PCI can substantially enhance the cytotoxicity of protein toxins as well as efficiency of gene transfection mediated by both non-viral and viral vectors.
There are several reasons why illumination before drug delivery should be advantageous as compared to previously discussed ‘light after’ strategy. Firstly, in most clinical situations it would be practically advantageous if the light-treatment and the administration of the drugs could be performed as simultaneously as possible. For example, employing the technology in combination with surgery, as an adjuvant treatment of residual disease after surgical removal of tumours, it would be a great advantage to apply both the light-treatment and the therapeutic macromolecule at the end of surgical procedures and immediately following each other. In this case the ‘light before’ approach would be clearly advantageous to the ‘light after’ strategy, where one would have to wait several hours to ensure maximal uptake of the drug. Secondly, the ‘light before’ strategy could be more optimal for delivery of intact macromolecules. Photochemical treatment can damage various biomolecules (Jori and Spikes, 1984). Therefore photochemical damage to the entrapped therapeutic molecules can not be ruled out when light is applied after the molecules, and this could decrease the positive effects of PCI. However, the primary photochemical effects should not directly damage molecules that are delivered into the cell after illumination. Thirdly, it is known that photochemical treatment can at least partially inactivate degradative lysosomal enzymes or impair microtubules involved in transport towards lysosomes (Berg and Moan, 1994, 1997), so that if applied in advance, it could protect endocytosed macromolecules from the possible lysosomal degradation by both these mechanisms.
In this study it has been shown that both the ‘light before’ and the ‘light after’ PCI-strategies improve the final biological effect of delivered macromolecules, however which approach leads to the better result seems to depend on the macromolecules to be delivered. Thus, in the case of gelonin it seems to be more beneficial to apply ‘light before’ gelonin (Figure 2). However, in the case of transgene, light-induced transfection mediated by both polylysine and adenovirus was similar regardless of whether illumination was performed before or after administration of the transgene (Figure 3). The difference in response between these two classes of molecules might be due to their different sensitivity, either to enzymatic degradation in endocytic vesicles, or to the photochemically induced damage. If gelonin is more sensitive to these damaging effects than the transgene (that might be protected by the carrier molecules: synthetic vectors or viruses), then the “‘light before’ approach might be especially beneficial due to the reasons discussed above. It should be mentioned that gelonin and reporter transgenes were used as examples to demonstrate the versatility and efficacy of the technology. However, the method is far from being optimized, and by use of other macromolecules under optimal conditions even better effects could be expected.
It has already been shown the efficiency of ‘light after’ PCI strategy in vivo in combination with gelonin (Selbo et al, 2001). The results described in the present work show that, in addition to the above mentioned practical advantages, the ‘light before’ strategy with gelonin in vitro works substantially more efficiently, making the presented new PCI concept worth further investigation. The exact cellular mechanism behind ‘light before’-based delivery is not known. One obvious possibility – the direct entry into the cytosol through the plasma membrane of photochemically treated cells – does not seem very likely, since the results argue against photochemical permeabilization of the plasma membrane and strongly indicate the involvement of endocytic transport.
Another alternative is endosomal release mediated by passive diffusion. As discussed above, photochemical treatment, if applied in advance, could protect endocytosed macromolecules from lysosomal degradation, so that functional macromolecules could release from intact endocytic vesicles via slow diffusion. However, the very rapid relocalization of FITC-dextran (Figure 1B) strongly suggests the existence of other mechanisms than slow passive diffusion responsible for PCI effect.
Thus, the likely mechanism behind ‘light before’ effects could be a fusion between photochemically ruptured vesicles and intact vesicles carrying the macromolecules, leading to endosomal release of the molecules as described in Figure 5B. More detailed studies of the light effects on transgene delivery revealed that for the ‘light before’ approach the best result was obtained when light was applied right before the DNA complex, while introducing a chase period between illumination and the DNA-treatment reduces the transfection efficiency (Figure 4). Possible reasons for this can be either cellular repair or removal of photochemically disrupted vesicles, so that the DNA is not redirected into the cytosol, but rather stays in intact vesicles. Work is in progress in our laboratory to prove these hypotheses.
The technology presented in this study represents an important addition to the emerging field of physically induced drug delivery methods. There are several factors making the PCI-technology a relatively tumour-specific method: (i) light, activating photosensitizers and inducing photochemical reactions, can be directed precisely to tumours; (ii) photosensitizers accumulate preferentially in tumours as compared to normal tissue (Chan et al, 1990; Pass, 1993). It should also be emphasized that there is already significant clinical experience employing photochemical principles, namely in photodynamic therapy (PDT), a quite specific approach used for treatment of different tumour types (Dougherty et al, 1998). In addition, the presented technology involves endocytosis and therefore it is very well suited for combination with other tumour-targeting strategies like surface-targeting via ligand-receptor interaction for specific uptake into tumour cells. Although the limited light penetration through the tissues might be a limitation, by the use of fibre optic devices it is possible to reach many sites also within the body, such as the gastrointestinal tract, lungs, brain etc. (Pass, 1993). Another factor, which might limit application of PCI technology is cytotoxicity, inducible by photochemical treatment, that was successfully exploited in PDT of cancer (Pass, 1993; Dougherty et al, 1998). In this respect, an attractive approach could be to use photochemically internalized therapeutic molecules (e.g. toxins or suicide genes) as adjuvants for PDT, to destroy cancer cells that were not efficiently killed by PDT. In this way deeper tumour layers, where due to limited light penetration a lower photochemical dose (consequently, lower toxicity) is obtained, could be also affected. However, in general, to balance photochemical dose is a very important task to exploit the full potential that photochemical internalization offers for different therapies.
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This study was supported by the Norwegian Research Council, Norwegian Cancer Society and The Norwegian Ministry of Health and Social Affairs.
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Prasmickaite, L., Høgset, A., Selbo, P. et al. Photochemical disruption of endocytic vesicles before delivery of drugs: a new strategy for cancer therapy. Br J Cancer 86, 652–657 (2002). https://doi.org/10.1038/sj.bjc.6600138
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