(A) Effect of the β3-AR antagonist SR59230A on lipolysis in murine white adipoctes; induced by LMF. Adipocytes were preincubated with the indicated concentration of SR59230A for 30 min prior to the addition of LMF (465 nM). (B) Effect of SR59230A on the stimulation of adenylate cyclase in isolated murine adipocyte plasma membranes by LMF. Membranes were preincubated with the indicated concentrations of SR59230A for 30 min prior to the addition of LMF (2.35 μM) and adenylate cyclase was determined as described in Materials and Methods in the presence of 0.1 μM GTP. The results are expressed as means ±s.d. and the data is representative of three separate experiments. Differences from incubation in the absence of SR59230A is indicated c, P<0.005 and d, P<0.001 as determined by Student's t-test.