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Development of a human mitochondria-focused cDNA microarray (hMitChip) and validation in skeletal muscle cells: implications for pharmaco- and mitogenomics

The Pharmacogenomics Journal volume 6, pages 333–342 (2006)Cite this article

Abstract

Mitochondrial research has influenced our understanding of human evolution, physiology and pathophysiology. Mitochondria, intracellular organelles widely known as ‘energy factories’ of the cell, also play fundamental roles in intermediary metabolism, steroid hormone and heme biosyntheses, calcium signaling, generation of radical oxygen species, and apoptosis. Mitochondria possess a distinct DNA (mitochondrial DNA); yet, the vast majority of mitochondrial proteins are encoded by the nuclear DNA. Mitochondria-related genetic defects have been described in a variety of mostly rare, often fatal, primary mitochondrial disorders; furthermore, they are increasingly reported in association with many common morbid conditions, such as cancer, obesity, diabetes and neurodegenerative disorders, although their role remains unclear. This study describes the creation of a human mitochondria-focused cDNA microarray (hMitChip) and its validation in human skeletal muscle cells treated with glucocorticoids. We suggest that hMitChip is a reliable and novel tool that will prove useful for systematically studying the contribution of mitochondrial genomics to human health and disease.

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Accession codes

Accessions

GenBank/EMBL/DDBJ

Abbreviations

ACADL:

acyl-Coenzyme A dehydrogenase, long chain

ACADM:

acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain

ACADVL:

acyl-Coenzyme A dehydrogenase, very long chain

AK1:

adenylate kinase 1

Dex:

dexamethasone

FACL2:

acyl-CoA synthetase long-chain family member 1

FDX1:

ferredoxin 1

hMitChip:

human mitochondria-focused cDNA microarray

MAOA:

monoamine oxidase A

mtDNA:

mitochondrial DNA

nDNA:

nuclear DNA

PPIB:

peptidylprolyl isomerase B (cyclophilin B)

QRT-PCR:

quantitative real-time polymerase chain reaction

ROS:

radical oxygen species.

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Acknowledgements

This research was supported by the Intramural Research Programs of the National Institute of Child Health and Human Development, the National Institute of Mental Health, and the National Center for Complementary and Alternative Medicine. The support of the Public Benefit Foundation ‘Alexander S Onassis’ to Dr I Manoli is gratefully acknowledged.

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Authors and Affiliations

  1. Clinical Neuroendocrinology Branch, NIMH, NIH, Bethesda, MD, USA

    S Alesci & P W Gold

  2. Reproductive Biology and Medicine Branch, NICHD, NIH, Bethesda, MD, USA

    S Alesci, V J Michopoulos, F M Brouwers & G P Chrousos

  3. Laboratory of Clinical Investigation, NCCAM, NIH, Bethesda, MD, USA

    I Manoli, H Le & M R Blackman

  4. First Department of Pediatrics, University of Athens, Athens, Greece

    I Manoli & G P Chrousos

  5. Laboratory of Clinical Genomics, NICHD, NIH, Bethesda, MD, USA

    O M Rennert

  6. Department of Pathology, Loyola University, Maywood, IL, USA

    Y A Su

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  1. S Alesci
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  2. I Manoli
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Correspondence to S Alesci.

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Supplementary Information accompanies the paper on the The Pharmacogenomics Journal website (http://www.nature.com/tpj).

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Alesci, S., Manoli, I., Michopoulos, V. et al. Development of a human mitochondria-focused cDNA microarray (hMitChip) and validation in skeletal muscle cells: implications for pharmaco- and mitogenomics. Pharmacogenomics J 6, 333–342 (2006). https://doi.org/10.1038/sj.tpj.6500377

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