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Analysis of gene expression in carbon tetrachloride-treated rat livers using a novel bioarray technology

The Pharmacogenomics Journal volume 3, pages 41–52 (2003)Cite this article

ABSTRACT

The present study successfully utilizes a new ADME Rat Expression Bioarray, containing 1040 metabolism- and toxicology-linked genes, to monitor gene expression from the livers of rats treated with carbon tetrachloride (CCl4). Histopathological analysis, hierarchical clustering methods, and gene expression profiling are compared between the control and CCl4-treated animals. A total of 44 transcripts were found to be altered in response to the hepatotoxin, 19 of which were upregulated and 25 were downregulated. Some of these gene expression changes were expected and concurred with previously published data while others were novel findings.

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Acknowledgements

We thank Sujal Shah, Scott Spear and Loey Healy for technical assistance, Ed Klein, Veterinary Diagnostic Associates, Murrysville, PA, for his support to the histopathology, Phillip Stafford for statistical assistance, and Bill LaFromboise and Mark Braughler for their contribution to the design of this study. We also thank Informax, Inc. (Bethesda, MD, USA) for providing software and support for this work.

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Authors and Affiliations

  1. Motorola Life Sciences, 4088 Commercial Avenue, Northbrook, IL, USA

    M B Young, T J Sendera & S R Magnuson

  2. TissueInformatics, Inc., Suite 202, Pittsburgh, PA, USA

    M R DiSilvestro, J Freund & A Kriete

  3. Amersham Biosciences, 3200 West Germann Rd., 72 85248, Chandler, USA

    T J Sendera

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  1. M B Young
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Correspondence to T J Sendera.

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Young, M., DiSilvestro, M., Sendera, T. et al. Analysis of gene expression in carbon tetrachloride-treated rat livers using a novel bioarray technology. Pharmacogenomics J 3, 41–52 (2003). https://doi.org/10.1038/sj.tpj.6500147

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