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Genome-wide linkage analysis of 160 North American families with celiac disease

Genes & Immunity volume 8, pages 108–114 (2007)Cite this article

Abstract

Celiac disease (CD) is a common autoimmune disease caused by exposure to the protein gliadin in wheat, and related prolamins in barley and rye. The prevalence of the disease in the US is 1:133. The aim of this study was to identify non-human leukocyte antigen (HLA) loci that predispose to CD. A genome-wide search of 405 microsatellite markers was performed on DNA samples from 160 families with a minimum of two cases of CD. Multipoint, parametric and non-parametric linkage (NPL) analyses were performed. Locations on chromosomes 1q, 3q, 6p, 6q, 7q, 9q and 10q showed linkage statistics (NPL scores or heterogeneity logarithm of the odds (HLOD) scores) of approximately 2.0 or larger. The greatest evidence for linkage outside of chromosome 6 was on 7q and 9q. An NPL score of 2.60 occurred at position 151.0 on 7q and a HLOD score of 2.47 occurred at position 144.8 on 9q under a recessive model. As expected, there was highly significant linkage to the HLA region on 6p, with NPL and HLOD scores exceeding 5.50. In conclusion, this genome-wide linkage analysis represents one of the largest such studies of CD. The most promising region is a putative locus on 7q, a region reported independently in previous genome-wide searches.

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Acknowledgements

This study was funded by NIH DK50678. We thank the families for participating in our study. We also thank Vu Huynh for technical assistance in the laboratory and Maryam Mousavi and Marie Pinto for working with the families. Genotyping services were provided by the Center for Inherited Disease Research (CIDR). CIDR is fully funded through a federal contract from the National Institutes of Health to The Johns Hopkins University, contract number N01-HG-65403.

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Authors and Affiliations

  1. Division of Epidemiology, Department of Medicine, University of California Irvine, Irvine, CA, USA

    C P Garner, Y C Ding, L Steele & S L Neuhausen

  2. Division of Pediatric Gastroenterology, Department of Pediatrics, University of Utah, Salt Lake City, UT, USA

    L Book

  3. Department of Dermatology, University of Utah, Salt Lake City, UT, USA

    K Leiferman & J J Zone

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  1. C P Garner
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Correspondence to S L Neuhausen.

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Garner, C., Ding, Y., Steele, L. et al. Genome-wide linkage analysis of 160 North American families with celiac disease. Genes Immun 8, 108–114 (2007). https://doi.org/10.1038/sj.gene.6364361

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