Effect of C5 status on different measures of susceptibility to candidiasis. (a) Heart fungal load was measured in 64 F2 mice 48 h after low dose infection with C. albicans. (b) Survival was monitored in 122 F2 mice for up to 28 days after infection with a high dose of C. albicans. (c) Serum TNF-α levels were measured 48 h after infection with a low dose of C. albicans in 56 F2 mice. For all phenotypes there was a significant linkage between C5-deficiency and an increased susceptibility to infection (***P<0.001). The response of A/J and B6 parents are included for comparison. C5 alleles are identified as ‘a’ and ‘b’, for A/J and B6, respectively, and were identified by genotyping (see Materials and methods). Horizontal bars indicate the mean value for each of the traits analyzed for each group.