Table 2 Oligonucleotides used in genomic, RT-PCR, and quantitative RT-PCR

From: Characterization of the human CD5 endogenous retrovirus-E in B lymphocytes

Primer Sequence   Acc no.
CD5 E1B sense 5′-CTTGGAGTCCGGACAACTGAAACTTG-3′ RT-PCR, quantitative AY541704
CD5 E1A sense 5′- CCTTGTACCTGCTGGGGATGCTG-3′ Quantitative AY541703
11q 12.2 gag antisense 5′-TGTGTCCGTCCTCATCTACCCCAG-3′ RT-PCR AP000437
11q 12.2 pro antisense 5′-CCATACTTGGGGCCATTGCTTAGC-3′ RT-PCR AP000437
11q 12.2 env antisense 5′-GTGGTCTCCTCCCTTATTCCCCTC-3′ RT-PCR AP000437
CD5 E5-6 antisense 5′-TGGAAACCTGAGCAAAGGAGGGCA-3′ RT-PCR NM_014207
CD5 E3 antisense 5′-TGCCATCCGTCCTTGAGGTAGAC-3′ Quantitative NM_014207
CD5 enh sense 5′-GACTTCCTCTTGTGTCATGGAAAGTGC-3′ Genomic PCR AP000437
CD5 P1A antisense 5′-TGTTTGAAGTTGAACTGTCAACTTCCTCC-3′ Genomic PCR AP000437
11q 12.2 5′LTR antisense 5′-GTGCCAGTTCCTTCCCTGTGTTCAGCCACT-3′ Genomic PCR AY541704
17q21.31 5′LTR sense 5′-CGGGCACTTGGAGTCTGGACATC-3′ RT-PCR BG255379
FLJ36232 E4 antisense 5′-TTGATCAGTTAGGGTGGAGCAGGAAC-3′ RT-PCR AK093551
7p22.1 5′LTR sense 5′-GGAAGGAATGGGCACTTGGAGTCTG-3′ RT-PCR BF762816
7p22.1 gag antisense 5′-CATAGTTCCTAGTGGGGTGGGCTTACT-3′ RT-PCR AC092032
2p24.3 5′LTR sense 5′-AGGATCAACACAGTGGCTGAACACC-3′ RT-PCR BX445808
HERV-L RT antisense 5′-AAGATCAGATGGTTGTAGATGCATGG-3′ RT-PCR  
19q13.32 5′LTR sense 5′-GGTCTGAGGACCCCACAGAGTCAG-3′ RT-PCR BC055093
ApoC1 E5 antisense 5′-ACCCTTCAGGTCCTCATGAGTCAATC-3′ RT-PCR BP304001
13q22.33 5′LTR sense 5′-GGGAAGGAACTGGTACTTGGAGTCTG-3′ RT-PCR CB994808
EDNRB E2 antisense 5′-AGGTCTTAGTGGGTGGCGTCATTATC-3′ RT-PCR  
7p14.3 5′LTR sense 5′-ATGTGGTGGCTGAACACCAGGAAG-3′ RT-PCR AL524147
FLJ22374 E11 antisense 5′-AAGGCTGCAGTCCCTGAGCACAG-3′ RT-PCR  
HERV-E 4.1 5′LTR sense 5′-AGGAACAGGCACTTGGAGTCCAGAC-3′ RT-PCR M74509
HERV-E 4.1 gag antisense 5′-GGTCTAGCACCAGCTGTAACCAAGTG-3′ RT-PCR  
GAPDH E2 sense 5′-GAAGGTGAAGGTCGGAGTC-3′ RT-PCR NM_002046
GAPDH E4 antisense 5′-GAAGATGGTGATGGGATTTC-3′ RT-PCR  
HPRT E6 sense 5′-TTGACACTGGCAAAACAATGCAGAC-3′ Quantitative NM_00094
HPRT E7 antisense 5′-GGGTCCTTTTCACCAGCAAGCT-3 Quantitative NM_00094
  1. E=exon.