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Cis-acting effect of the IL1B C−31T polymorphism on IL-1β mRNA expression

Genes & Immunity volume 5, pages 572–575 (2004)Cite this article

Abstract

Since the effect of IL1B polymorphisms on IL-1β production is still controversial, we selected two polymorphisms to test their cis-acting effect on IL-1β mRNA expression by means of the allele-specific transcript quantification and the haplotype analysis. As for the C−31T polymorphism, we found that expression of the −31T allele was 2.2 times of the −31C allele. This higher transcription efficiency may correspond to the fact that C−31T is located in a TATA box. The other polymorphism, C+3954T, did not alter the levels of transcription. The use of the allele-specific transcript quantification enables us to exclude trans-acting effects of polymorphisms on the gene expression and contributes to understanding the roles of the IL1B polymorphisms in susceptibility to multifactorial diseases.

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Acknowledgements

We are grateful to Dr M Hirai, Tokyo Women's Medical University, for providing DNA samples. This work was partly supported by the Japan Society for Promotion of Science and Ministry of Education, Culture, Sports, Science and Technology, Japan. This study constitutes a part of doctorial dissertation (RK) submitted to the University of Tokyo.

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  1. R Kimura

    Present address: Department of Human Genetics, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan

Authors and Affiliations

  1. Department of Biological Sciences, Unit of Human Biology and Genetics, Graduate School of Science, The University of Tokyo, Tokyo, Bunkyo-ku, Japan

    R Kimura, T Nishioka & T Ishida

  2. Department of Pediatrics, Faculty of Medicine, Diponegoro University, Semarang, Indonesia

    A Soemantri

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  1. R Kimura
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  2. T Nishioka
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Correspondence to T Ishida.

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Kimura, R., Nishioka, T., Soemantri, A. et al. Cis-acting effect of the IL1B C−31T polymorphism on IL-1β mRNA expression. Genes Immun 5, 572–575 (2004). https://doi.org/10.1038/sj.gene.6364128

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