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Murine susceptibility to Chagas' disease maps to chromosomes 5 and 17

Genes & Immunity volume 4, pages 321–325 (2003)Cite this article

Abstract

Chagas' disease is caused by the protozoan parasite Trypanosoma cruzi and commonly modelled in inbred mice. Susceptibility of mouse strains to experimental infection varies considerably. We quantified parasite tissue burdens in resistant and susceptible strains by real time PCR and applied a backcross strategy to map the genomic loci linked to susceptibility in inbred mice. Resistant B6D2F1 mice were backcrossed with susceptible C57BL/6 mice, and 46 of a total 192 offspring died after infection. Their genomes were scanned with microsatellite markers. One region on chromosome 17 was significantly linked to susceptibility, while another on chromosome 5 was suggestive of linkage.

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Acknowledgements

We are grateful to Y Richter for mouse care and breeding, and to I Gaworski, U Klauenberg, E Kist, D Pachale and R Martin for technical assistance.

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Authors and Affiliations

  1. Department of Immunology, Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany

    S E B Graefe, C Drosten, C Steeg & B Fleischer

  2. Max-Delbrück-Center, Gene Mapping Center, Berlin-Buch, Germany

    B S Meyer, F Rüschendorf & P Nürnberg

  3. Department of Bioinformatics, Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany

    B Müller-Myhsok

  4. Artus GmbH, Nobistor 29, Hamburg, Germany

    T Laue

Authors
  1. S E B Graefe
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  2. B S Meyer
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  3. B Müller-Myhsok
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  4. F Rüschendorf
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  5. C Drosten
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  6. T Laue
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  7. C Steeg
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  8. P Nürnberg
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  9. B Fleischer
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Correspondence to S E B Graefe.

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Graefe, S., Meyer, B., Müller-Myhsok, B. et al. Murine susceptibility to Chagas' disease maps to chromosomes 5 and 17. Genes Immun 4, 321–325 (2003). https://doi.org/10.1038/sj.gene.6363972

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  • DOI: https://doi.org/10.1038/sj.gene.6363972

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