The enzyme Cas9 is used in genome editing to cut selected DNA sequences, but it also creates breaks at off-target sites. Protein engineering has now been used to make Cas9 enzymes that have minimal off-target effects. See Article p.490
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The author declares competing financial interests: F.U. is a full-time employee of Sangamo BioSciences, Inc.
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Urnov, F. The domestication of Cas9. Nature 529, 468–469 (2016). https://doi.org/10.1038/529468a
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