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Synthetic biology

Construction of a yeast chromosome

One aim of synthetic biology is to generate complex synthetic organisms. Now, a stage in this process has been achieved in yeast cells — an entire yeast chromosome has been converted to a synthetic sequence in a stepwise manner.

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Figure 1: Building synthetic genomes.

References

  1. Gibson, D. G. et al. Science 329, 52–56 (2010).

    CAS  Article  ADS  Google Scholar 

  2. Annaluru, N. et al. Science 344, 55–58 (2014).

    CAS  Article  ADS  Google Scholar 

  3. Gibson, D. G. et al. Proc. Natl Acad. Sci. USA 105, 20404–20409 (2008).

    CAS  Article  ADS  Google Scholar 

  4. Gibson, D. G. et al. Science 319, 1215–1220 (2008).

    CAS  Article  ADS  Google Scholar 

  5. Gibson, D. G. et al. Nature Methods 6, 343–345 (2009).

    CAS  Article  Google Scholar 

  6. Annaluru, N. et al. Methods Mol. Biol. 852, 77–95 (2012).

    CAS  Article  Google Scholar 

  7. Isaacs, F. J. et al. Science 333, 348–353 (2011).

    CAS  Article  ADS  Google Scholar 

  8. Glass, J. I., Hutchison, C. A. III, Smith, H. O. & Venter, J. C. Mol. Syst. Biol. 5, 330 (2009).

    Article  Google Scholar 

  9. Lartigue, C. et al. Science 325, 1693–1696 (2009).

    CAS  Article  ADS  Google Scholar 

  10. Lartigue, C. et al. Science 317, 632–638 (2007).

    CAS  Article  ADS  Google Scholar 

Download references

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Correspondence to Daniel G. Gibson.

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Gibson, D., Venter, J. Construction of a yeast chromosome. Nature 509, 168–169 (2014). https://doi.org/10.1038/509168a

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