Tiny thermometers used in living cells

Nanometre-scale thermometers that operate with millikelvin sensitivity have now been made from diamond crystals. The devices have been used to measure temperature gradients in living cells. See Letter p.54

Despite many promising studies, taking temperature measurements of environments at nanometre-scale resolution remains a formidable challenge. On page 54 of this issue, Kucsko et al.1 report a precious solution to this problem: a thermometer based on diamond nanocrystals, also known as nanodiamonds. This sensing tool could have many applications, ranging from studies of cell biology to measurements of nanoscale chemical reactions.

Temperature affects diverse physical phenomena. For example, changes in Earth's temperature patterns can lead to the formation of severe storms, droughts and floods. Temperature governs the kinetics, activation and equilibrium states of chemical reactions. And in humans, body temperature is precisely controlled, so that any deviation from the normal range triggers a cascade of biomolecular mechanisms to restore the body's equilibrium. Scientists have therefore developed a range of precise temperature-measuring tools — from satellites to infrared cameras, and a variety of more familiar thermometers — to measure temperature over length scales from multiple kilometres to submillimetres. But how can we measure temperature at length scales of a few micrometres, or a few tens of nanometres?

Kucsko and co-workers' approach is to use the unique properties of electron spins associated with single-nitrogen-atom impurities in diamonds. The presence of a nitrogen atom in a diamond's carbon-atom lattice creates a point defect called a nitrogen vacancy (NV) centre, in which the nitrogen and a vacancy replace two neighbouring carbons. The ground state of an NV centre is split into two energy levels: the spin state of the lower level is 0, whereas that of the higher level is 1. The energy difference between the levels, known as the ground-state energy gap, is highly sensitive to temperature because it varies in response to thermally induced lattice strains. The principle of diamond thermometry is based on accurate measurement of changes in the transition frequency associated with this energy gap — the microwave frequency that corresponds to the energy difference between the lower and higher levels.

In their technique, Kucsko and colleagues used green light to excite electrons in NV centres, which then decayed to the ground state by emitting red fluorescence. The intensity of the fluorescence depends on the spin state of the NV centres. The authors also irradiated their nanodiamonds with microwaves to modulate the electron occupancy of the ground spin states 0 and 1, and determined the occupancy of the states from the observed fluorescence. They then used this information to work out the changes in the ground-state energy gap that are associated with temperature variations.

The researchers first used an isotopically pure (carbon-12 isotope) bulk diamond sample to determine the ultimate sensitivity of their NV-based thermometry. In this system, they detected temperature changes with an accuracy of up to 1.8 millikelvins under ideal experimental conditions. Similar sensitivity has just been reported by other groups2,3 using analogous experimental techniques and conditions.

However, Kucsko et al. went further by demonstrating how nanodiamond thermometers can measure the temperatures in living cells (Fig. 1). They used a clever nanowire-assisted delivery method4 to position nanodiamonds and gold nanoparticles inside the cells. When excited by laser light, the gold nanoparticles acted as localized heat sources. By using their technique to measure sub-kelvin temperature changes inside a single cell, the authors directly monitored the amount of heat generated by a single gold nanoparticle that was required to kill the cell.

Figure 1: Temperature measurements in living cells.

When diamond-lattice defects known as nitrogen vacancy (NV) centres are excited by green light, they emit red fluorescence. Kucsko et al.1 inserted nanodiamonds into single living cells, and irradiated them with microwaves to modulate the electron occupancy of spin states in NV centres, and with green light. By measuring the fluorescence from these centres, the authors established the electron occupancy of the spin states, and so determined changes in the ground-state energy gap (the microwave frequency that corresponds to the energy difference between spin states) that are associated with temperature variations. In this way, they measured the temperature gradient generated when a gold nanoparticle in the cell was heated by a laser beam, achieving sub-kelvin sensitivity.

Kucsko and colleagues' nanodiamond temperature sensors have high spatial resolution together with sub-kelvin thermal sensitivity, chemical inertness, biocompatibility and the best-known thermal conductivity of all solid materials. This is an ideal blend for a nanothermometer. Furthermore, temperature sensing with nanodiamonds could be extended to in vivo applications if a different method for fluorescence excitation were adopted: microwave excitation of electronic spin states has already been carried out in animals5, and the use of 'two-photon' excitation would allow the analysis of deeper tissue than could be achieved with the present method.

Because nanodiamonds are discrete objects, however, the authors' method can take measurements only at distinct locations, rather than taking continuous measurements of a temperature field. Furthermore, the method monitors temperature variations rather than absolute temperature. The authors suggest that this limitation could be overcome by using ensembles of nanodiamonds or diamond samples in which the lattices have low strain, either of which would reduce the experimental variations that currently limit absolute temperature from being measured. At present, the technique also has a fairly low temporal resolution of tens of seconds. This is sufficient for measurements of many biological processes, such as changes in gene expression, but is too slow for studies of temperature effects in faster processes, for example the initial steps of signal transduction, or neural activity.

How might this new tool further our understanding of human cell biology, or enable biomedical advances? Most human cells are 10–20 micrometres in size and are highly compartmentalized by internal membranes that separate cellular organelles. These organelles create multiple micrometre-sized reactors in which a plethora of energy-producing and energy-absorbing reactions occur. The reactions generate intracellular temperature gradients on micrometre and submicrometre scales that, in turn, influence other cellular biochemical reactions. Furthermore, external biochemical signalling and environmental changes activate molecular responses inside cells that can lead to corresponding changes in intracellular temperature gradients. The ability to measure intracellular temperature precisely would therefore provide an invaluable tool for cellular biophysicists, potentially allowing cellular behaviour and characteristics to be manipulated by controlling the temperature within, or close to, cellular organelles.

Kucsko and co-workers' technique could also open up many other intriguing topics for research, including the thermal modulation of immune responses6, molecular mechanisms of therapeutic tissue preservation induced by local cooling7, the role of subcellular temperature gradients in cell function8, and cell resistance to hyperthermia treatment9 (deliberately induced elevated body temperature, used, for example, as anticancer therapy). When it comes to measuring temperature, it may be that diamonds are a scientist's best friend.


  1. 1

    Kucsko, G. et al. Nature 500, 54–58 (2013).

    CAS  Article  ADS  Google Scholar 

  2. 2

    Toyli, D. M., de las Casas, C. F., Christle, D. J., Dobrovitski, V. V. & Awschalom, D. D. Proc. Natl Acad. Sci. USA 110, 8417–8421 (2013).

    CAS  Article  ADS  Google Scholar 

  3. 3

    Neumann, P. et al. Nano Lett. 13, 2738–2742 (2013).

    CAS  Article  ADS  Google Scholar 

  4. 4

    Shalek, A. K. et al. Proc. Natl Acad. Sci. USA 107, 1870–1875 (2010).

    CAS  Article  ADS  Google Scholar 

  5. 5

    Han, J. Y., Hong, J. T. & Oh, K.-W. Arch. Pharm. Res. 33, 1293–1299 (2010).

    CAS  Article  Google Scholar 

  6. 6

    Frey, B. et al. Int. J. Hyperthermia 28, 528–542 (2012).

    CAS  Article  Google Scholar 

  7. 7

    Geva, A. & Gray, J. Med. Decision Making 32, 266–272 (2012).

    Article  Google Scholar 

  8. 8

    Yang, J.-M., Yang, H. & Lin, L. ACS Nano 5, 5067–5071 (2011).

    CAS  Article  Google Scholar 

  9. 9

    Rylander, M. N., Feng, Y., Zimmermann, K. & Diller, K. R. Int. J. Hyperthermia 26, 748–764 (2010).

    CAS  Article  Google Scholar 

Download references

Author information



Corresponding author

Correspondence to Konstantin Sokolov.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Sokolov, K. Tiny thermometers used in living cells. Nature 500, 36–37 (2013).

Download citation


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.


Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing