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Biotechnology

Rewriting a genome

A bacterial enzyme that uses guide RNA molecules to target DNA for cleavage has been adopted as a programmable tool to site-specifically modify genomes of cells and organisms, from bacteria and human cells to whole zebrafish.

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Figure 1: Targeted genome editing with RNA-guided Cas9.

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Correspondence to Emmanuelle Charpentier or Jennifer A. Doudna.

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Charpentier, E., Doudna, J. Rewriting a genome. Nature 495, 50–51 (2013). https://doi.org/10.1038/495050a

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