Marine algae known as coccolithophores produce much of the ocean's calcium carbonate. A large survey reveals how these organisms' calcification processes and species distribution change in response to carbon dioxide levels. See Letter p.80
Coccolithophores are humble marine phytoplankton that are the subject of a simmering controversy in marine science concerning their response to ocean acidification. On page 80 of this issue, Beaufort et al.1 report a finding that should help settle the matter: coccolithophores produce thinner calcium carbonate shells as oceans become more acidic. But the mechanisms involved, and an unexpected exception to the general rule, may surprise those studying global change.
Over the past 220 million years or so, coccolithophores have performed a unique dual function in the ocean's carbon cycle. Like all phytoplankton, coccolithophores make their living by converting dissolved inorganic carbon in sea water into organic carbon through photosynthesis. But they also have a singular ability to use dissolved inorganic carbon to produce a mineral shell consisting of coccoliths, overlapping plates of calcium carbonate (CaCO3). Although the alga's long evolutionary history spans several major fluctuations in atmospheric carbon dioxide content, predicting the responses of coccolithophore calcification to ocean acidification — the anthropogenic enrichment of the modern ocean with CO2 — has been anything but straightforward.
The question of how coccolithophore calcification will respond to future high CO2 conditions has big implications for the ocean's carbon cycle, and perhaps also for global climate. The ratio of CaCO3 to organic carbon in the continuous 'rain' of biogenic particles that sink down from the ocean's surface (the 'rain ratio'2) is a key factor in carbon biogeochemical models, for several reasons3. One of the most important is that, in contrast to the photosynthetic production of organic carbon, which consumes CO2, the calcification reaction produces CO2 by converting two bicarbonate ions (HCO3−) into one CaCO3 and one CO2 molecule (Fig. 1). Thus, the concentration of CO2 in sea water is sensitive to changes in the rain ratio, which is a proxy for the amount of calcification versus photosynthesis occurring in the ocean.
The link between calcification and fluxes of climate-altering CO2 prompted experiments that found that the production of CaCO3 was reduced in coccolithophores growing at elevated CO2 levels4,5,6. Given that future ocean CO2 concentrations are expected to be high, concomitant reductions in calcification would lower the rain ratio, potentially helping to counter the rise in atmospheric CO2 concentrations. But just as marine scientists were becoming comfortable with this emerging ocean-acidification model, other papers7,8 stirred the pot by reporting that high CO2 levels increase the amount of CaCO3 produced by coccolithophore cells.
Beaufort et al.1 have now boldly charged into the resulting confusion and dissension. Unlike the previous studies, their work did not manipulate CO2 levels in cultures4,6 or in natural communities9,10 of coccolithophores. Instead, they used image-analysis techniques to determine the masses of more than half a million individual coccoliths from hundreds of modern surface seawater samples and from ancient marine sediment cores, collected from all over the world. They also measured the corresponding concentrations of dissolved inorganic carbon in the modern seawater samples, or calculated these concentrations for the sediment cores using accepted palaeoceanographic proxies.
Their findings are unequivocal: as CO2 concentration increases, coccolith mass declines in a more or less linear fashion. This relationship holds up regardless of the large local variations in seawater CO2 concentrations found in today's oceans, and it also holds up over long-term temporal fluctuations in atmospheric CO2, such as those that have occurred over glacial–interglacial cycles. The results seem to offer solid support for the hypothesis that coccolithophore cells will be less calcified in the future acidified ocean. But there is another twist to the story.
Beaufort et al. point out1 that the variations in coccolith mass measured in their study are much larger than the decreases in cellular CaCO3 typically observed when single species of coccolithophores are grown in culture at high CO2 concentrations. In fact, much of the coccolith-mass variability they recorded was apparently the result of taxonomic shifts in the coccolithophore community, rather than the result of reduced calcification within individual species. As levels of CO2 in sea water increase, assemblages of the algae progressively shift away from larger, heavily calcified species and towards smaller, lightly calcified ones. This trend occurs even within species, so that robustly calcified strains or morphotypes are replaced by more delicately calcified ones as CO2 levels rise. The authors' results therefore seem to imply that seawater carbonate chemistry is a strong selective force determining the taxonomic composition of coccolithophore communities.
So does this mean that the previously reported observations of increased cellular calcification in cultured coccolithophores at elevated CO2 concentrations were simply wrong? Not necessarily. Provocatively, Beaufort et al.1 also discovered one particular coccolithophore morphotype in their modern data set that goes decidedly against the general trend. This strain became much more heavily calcified as CO2 levels increased and as pH decreased along a sampling transect that ranged from the open ocean to coastal upwelling waters. The strain seems to be genetically similar to the widely distributed coccolithophore morphotype used in the controversial culture studies7,8.
This surprising exception to the rule raises new questions. For instance, if there are common strains of coccolithophores that thrive and calcify more at high CO2 concentrations, why don't they always dominate where seawater CO2 is elevated? (They obviously don't do this, because if they did, they would have obscured the strong negative correlation between CO2 and calcification observed by Beaufort et al.1.) The most likely reason is that many unknown factors also influence the abundance and calcification of coccolithophores. In fact, despite decades of intensive research effort, the environmental controls on coccolithophore growth are probably less well understood than for almost any other major phytoplankton group. What is clear, however, is that the environmental controls involved include many of the same factors that will also change concurrently with CO2 levels and pH under future global-change scenarios, such as temperature, visible and ultraviolet light intensity, and the availability of nutrients and trace elements11.
The next challenge for marine scientists is to try to understand how coccolithophore calcification and ecology will respond to evolutionary selection induced by this complex web of simultaneously changing environmental variables. Only then will we be able to predict what the net outcome will be for the future rain ratio of the ocean, and for the enigmatic phytoplankton group that drives it.
Beaufort, L. et al. Nature 476, 80–83 (2011).
Paasche, E. Physiol. Plant. Suppl. 3, 1–82 (1964).
Ridgwell, A. et al. Biogeosciences 6, 2611–2623 (2009).
Riebesell, U. et al. Nature 407, 364–367 (2000).
Zondervan, I., Zeebe, R. E., Rost, B. & Riebesell, U. Glob. Biogeochem. Cycles 15, 507–516 (2001).
Feng, Y. et al. Eur. J. Phycol. 43, 87–98 (2008).
Iglesias-Rodriguez, M. D. et al. Science 320, 336–340 (2008).
Shi, D., Xu, Y. & Morel, F. M. M. Biogeosciences 6, 1199–1207 (2009).
Engel, A. et al. Limnol. Oceanogr. 50, 493–507 (2005).
Feng, Y. et al. Mar. Ecol. Prog. Ser. 388, 13–25 (2009).
Boyd, P. W., Strzepek, R., Fu, F. & Hutchins, D. A. Limnol. Oceanogr. 55, 1353–1376 (2010).
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