Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Biological imaging

Beyond fluorescence

Nanoparticles that generate light through a mechanism known as second harmonic generation have been used to image live tissue. The particles overcome many problems associated with fluorescent probes for bioimaging.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Figure 1: One photon or two?


  1. Pantazis, P., Maloney, J., Wu, D. & Fraser, S. E. Proc. Natl Acad. Sci. USA 107 , 14535–14540 (2010).

  2. Nuriya, M. et al. Proc. Natl Acad. Sci. USA 103, 786–790 (2006).

    Article  ADS  CAS  Google Scholar 

  3. Salafsky, J. S. & Cohen, B. J. Phys. Chem. B 112, 15103–15107 (2008).

    Article  CAS  Google Scholar 

  4. Hsieh, C. L., Pu, Y., Grange, R. & Psaltis, D. Opt. Express 18, 11917–11932 (2010).

    Article  ADS  CAS  Google Scholar 

  5. Nakayama, Y. et al. Nature 447, 1098–1101 (2007).

    Article  ADS  CAS  Google Scholar 

  6. Park, Y. I. et al. Adv. Mater. 21, 4467–4471 (2009).

    Article  CAS  Google Scholar 

  7. Wu, S. et al. Proc. Natl Acad. Sci. USA 106, 10917–10921 (2009).

    Article  ADS  CAS  Google Scholar 

  8. Salthouse, C., Hilderbrand, S., Weissleder, R. & Mahmood, U. Opt. Express 16, 21731–21737 (2008).

    Article  ADS  Google Scholar 

  9. Allen, P. M. et al. J. Am. Chem. Soc. 132, 470–471 (2010).

    Article  CAS  Google Scholar 

  10. Wang, F. et al. Nature 463, 1061–1065 (2010).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations


Rights and permissions

Reprints and permissions

About this article

Cite this article

Cohen, B. Beyond fluorescence. Nature 467, 407–408 (2010).

Download citation

  • Published:

  • Issue Date:

  • DOI:

This article is cited by


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing