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Microscopy

Light from the dark

Fluorescence microscopy is the most popular way to image biomolecules, but it leaves many of them in the dark. Non-fluorescent, light-absorbing molecules can now be viewed by a method that turns them into mini-lasers.

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Figure 1: Imaging non-fluorescent molecules using stimulated emission.

References

  1. Min, W. et al. Nature 461, 1105–1109 (2009).

    Article  ADS  CAS  Google Scholar 

  2. Schäfer, F. P. Dye Lasers (Springer, 1973).

    Google Scholar 

  3. Schawlow, A. L. & Townes, C. H. Phys. Rev. 112, 1940–1949 (1958).

    Article  ADS  CAS  Google Scholar 

  4. Hell, S. W. & Wichmann, J. Opt. Lett. 19, 780–782 (1994).

    Article  ADS  CAS  Google Scholar 

  5. Fu, D. et al. J. Biomed. Opt. 12, 054004 (2007).

    Article  ADS  Google Scholar 

  6. Freudiger, C. W. et al. Science 322, 1857–1861 (2008).

    Article  ADS  CAS  Google Scholar 

  7. Dong, C. Y., So, P. T. C., Buehler, C. & Gratton, E. Optik 106, 7–14 (1997).

    Google Scholar 

  8. Hell, S. W. Nature Biotechnol. 21, 1347–1355 (2003).

    Article  CAS  Google Scholar 

Download references

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Hell, S., Rittweger, E. Light from the dark. Nature 461, 1069–1070 (2009). https://doi.org/10.1038/4611069a

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