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Notch signalling and voltage-gated Na+ channel activity in human prostate cancer cells: independent modulation of in vitro motility

Prostate Cancer and Prostatic Diseases volume 9, pages 399–406 (2006)Cite this article

Abstract

This study tested the possible functional relationship of two signalling mechanisms shown previously to be involved in human prostate cancer (PCa), Notch and voltage-gated sodium channel. Notch1 and Notch2 were differentially expressed in PCa cell lines of varying metastatic potential (LNCaP, PC-3, PC-3M) in comparison to a normal prostate cell line (PNT2), whereas Notch3 and Notch4 were not expressed. The Notch ligand Jagged1, but not Jagged2, was increased in all cell lines, whereas the Notch downstream target Deltex was not expressed. In comparison to the LNCaP cell line, Hes1, another downstream target, showed elevated expression in the metastatic PC-3 and PC-3M cells and promoted lateral motility. In contrast, the Notch ligand Delta-like1 (Dll1) levels were higher in LNCaP compared with PC-3 and PC-3M cells. Importantly, decreasing Dll1 expression increased the lateral motility of PC-3 cells, whereas blocking voltage-gated Na+ channel activity with tetrodotoxin decreased motility. However, the effect of Dll1 was independent of Notch signalling through Hes1 and voltage-gated Na+ channel expression/activity.

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Acknowledgements

This study was supported by the Pro Cancer Research Fund (MBAD, SPF) and NKRF (MJD). We thank James KJ Diss for useful discussion and Professor Norman Maitland (supported by Yorkshire Cancer Research) for the PNT2 cell line.

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Authors and Affiliations

  1. Division of Cell and Molecular Biology, Neuroscience Solutions to Cancer Research Group, Imperial College London, South Kensington Campus, London, UK

    N Scorey, S P Fraser, P Patel & M B A Djamgoz

  2. Division of Immunity and Infection, Section of Immunity and Infection, Imperial College London, South Kensington Campus, London, UK

    N Scorey, P Patel, C Pridgeon & M J Dallman

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  1. N Scorey
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Correspondence to S P Fraser.

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Scorey, N., Fraser, S., Patel, P. et al. Notch signalling and voltage-gated Na+ channel activity in human prostate cancer cells: independent modulation of in vitro motility. Prostate Cancer Prostatic Dis 9, 399–406 (2006). https://doi.org/10.1038/sj.pcan.4500894

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