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The effects of curcumin on the invasiveness of prostate cancer in vitro and in vivo


Curcumin has become a focus of interest with regard to its antitumor effects in prostate cancer; however, the effects of this agent on invasion and metastasis remain less well understood. Matrix metalloproteinases (MMPs) are important prerequisite for tumor invasion and metastasis. In this study, we evaluated the effects of curcumin on prostate cancer cells (DU-145) invasion in both in vitro and in vivo. We utilized zymography and ELISA in order to determine the MMP-2 and MMP-9 activity. Matrigel invasion assay was performed to assess cellular invasion. We developed a xenograft model to examine tumorigenicity. Curcumin treatment resulted not only in a significant reduction in the expression of MMP-2 and MMP-9, but also effected the inhibition of invasive ability in vitro. Curcumin was shown to induce a marked reduction of tumor volume, MMP-2, and MMP-9 activity in the tumor-bearing site. The metastatic nodules in vivo were significantly fewer in the curcumin-treated group than untreated group. Curcumin appears to constitute a potential agent for the prevention of cancer progression, or at least of the initial phase of metastasis, in prostate cancer.

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  1. Jemal A, Tiwari RC, Murray T, Ghafoor A, Samuels A, Ward E et al. Cancer statistics, 2004. CA Cancer J Clin 2004; 54: 8–29.

    Article  Google Scholar 

  2. Denis L, Morton MS, Griffiths K . Diet and its preventive role in prostatic disease. Eur Urol 1999; 35: 377–387.

    Article  CAS  Google Scholar 

  3. Hebert JR, Hurley TG, Olendzki BC, Teas J, Ma Y, Hampl JS . Nutritional and socioeconomic factors in relation to prostate cancer mortality: a cross-national study. J Natl Cancer Inst 1998; 90: 1637–1647.

    Article  CAS  Google Scholar 

  4. Yu AE, Hewitt RE, Connor EW, Stetler-Stevenson WG . Matrix metalloproteinases. Novel targets for directed cancer therapy. Drugs Aging 1997; 11: 229–244.

    Article  CAS  Google Scholar 

  5. Kohn EC, Liotta LA . Molecular insights into cancer invasion: strategies for prevention and intervention. Cancer Res 1995; 55: 1856–1862.

    PubMed  CAS  Google Scholar 

  6. John A, Tuszynski G . The role of matrix metalloproteinases in tumor angiogenesis and tumor metastasis. Pathol Oncol Res 2001; 7: 14–23.

    Article  CAS  Google Scholar 

  7. Stearns M, Stearns ME . Evidence for increased activated metalloproteinase 2 (MMP-2a) expression associated with human prostate cancer progression. Oncol Res 1996; 8: 69–75.

    PubMed  CAS  Google Scholar 

  8. Kanoh Y, Akahoshi T, Ohara T, Ohtani N, Mashiko T, Ohtani S et al. Expression of matrix metalloproteinase-2 and prostate-specific antigen in localized and metastatic prostate cancer. Anticancer Res 2002; 22: 1813–1817.

    PubMed  CAS  Google Scholar 

  9. Trudel D, Fradet Y, Meyer F, Harel F, Tetu B . Significance of MMP-2 expression in prostate cancer: an immunohistochemical study. Cancer Res 2003; 63: 8511–8515.

    PubMed  CAS  Google Scholar 

  10. Zhang L, Shi J, Feng J, Klocker H, Lee C, Zhang J . Type IV collagenase (matrix metalloproteinase-2 and -9) in prostate cancer. Prostate Cancer Prostatic Dis 2004; 7: 327–332.

    Article  CAS  Google Scholar 

  11. Morgia G, Falsaperla M, Malaponte G, Madonia M, Indelicato M, Travali S et al. Matrix metalloproteinases as diagnostic (MMP-13) and prognostic (MMP-2, MMP-9) markers of prostate cancer. Urol Res 2005; 33: 44–50.

    Article  CAS  Google Scholar 

  12. Dorai T, Gehani N, Katz A . Therapeutic potential of curcumin in human prostate cancer-I. Curcumin induces apoptosis in both androgen-dependent and androgen-independent prostate cancer cells. Prostate Cancer Prostatic Dis 2000; 3: 84–93.

    Article  CAS  Google Scholar 

  13. Dorai T, Cao YC, Dorai B, Buttyan R, Katz AE . Therapeutic potential of curcumin in human prostate cancer. III. Curcumin inhibits proliferation, induces apoptosis, and inhibits angiogenesis of LNCaP prostate cancer cells in vivo. Prostate 2001; 47: 293–303.

    Article  CAS  Google Scholar 

  14. Mukhopadhyay A, Bueso-Ramos C, Chatterjee D, Pantazis P, Aggarwal BB . Curcumin downregulates cell survival mechanisms in human prostate cancer cell lines. Oncogene 2001; 20: 7597–7609.

    Article  CAS  Google Scholar 

  15. Dorai T, Dutcher JP, Dempster DW, Wiernik PH . Therapeutic potential of curcumin in prostate cancer-V: interference with the osteomimetic properties of hormone refractory C4-2B prostate cancer cells. Prostate 2004; 60: 1–17.

    Article  CAS  Google Scholar 

  16. Shenouda NS, Zhou C, Browning JD, Ansell PJ, Sakla MS, Lubahn DB et al. Phytoestrogens in common herbs regulate prostate cancer cell growth in vitro. Nutr Cancer 2004; 49: 200–208.

    Article  CAS  Google Scholar 

  17. Adams BK, Cai J, Armstrong J, Herold M, Lu YJ, Sun A et al. EF24, a novel synthetic curcumin analog, induces apoptosis in cancer cells via a redox-dependent mechanism. Anticancer Drugs 2005; 16: 263–275.

    Article  CAS  Google Scholar 

  18. Nakamura K, Yasunaga Y, Segawa T, Ko D, Moul JW, Srivastava S et al. Curcumin down-regulates AR gene expression and activation in prostate cancer cell lines. Int J Oncol 2002; 21: 825–830.

    PubMed  CAS  Google Scholar 

  19. Sehgal G, Hua J, Bernhard EJ, Sehgal I, Thompson TC, Muschel RJ . Requirement for matrix metalloproteinase-9 (gelatinase B) expression in metastasis by murine prostate carcinoma. Am J Pathol 1998; 152: 591–596.

    PubMed  PubMed Central  CAS  Google Scholar 

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This research was conducted with financial support provided by the OTTOGI Corporation, Anyang, Korea.

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Correspondence to H Y Choi.

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Hong, J., Ahn, K., Bae, E. et al. The effects of curcumin on the invasiveness of prostate cancer in vitro and in vivo. Prostate Cancer Prostatic Dis 9, 147–152 (2006).

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  • curcumin
  • animal model
  • matrix metalloproteinases

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