Innate immune defences are our first line of protection against infection by viruses and are essential in limiting viral disease. But their reaction to the 1918 influenza virus could have been deadly.
The devastating Spanish influenza A virus infected around a third of the world's population during the pandemic of 1918. With mortality rates more than 25 times that of other influenza pandemics, the 1918 virus killed over 40 million people worldwide1. Influenza A virus is transmitted among humans and domestic animals, and when different strains infect the same cell, mixing of the two viral genomes (or 'reassortment') can generate new strains with epidemic or pandemic potential.
Studies of genetically engineered influenza A viruses containing some or all of the genes from the 1918 virus suggest that this virus might have acquired a unique combination of genes that caused the disease's unusual severity1,2. However, the specific actions of these genes and their contribution to the virulence of the 1918 pandemic have remained elusive. On page 319 of this issue, Kobasa et al.3 provide the first report of the effects of the reconstructed 1918 influenza virus in monkeys. Their findings link the unprecedented lethality of the 1918 pandemic virus to an aberrant innate immune response.
During infection, an invading virus is recognized by specialized cellular proteins that engage viral nucleic acids or proteins and trigger signalling pathways within the infected cell. These pathways culminate in the production of molecules of the innate immune system — our first line of protection against infection — called α/β-interferons, proinflammatory cytokines and chemokines4. This group of proteins includes interleukins, which are essential for activating immune cells, and chemoattractant proteins, which recruit immune cells to sites of infection. The innate immune response is triggered over a course of minutes to hours after infection, and these secreted proteins act collectively, both locally and systemically, to direct the expression of a wide range of proteins that act directly against the virus or stimulate inflammation. However, unchecked or excessive stimulation of the innate immune response can be harmful. It can contribute to the virulence of pathogenic viruses, in part by causing excessive infiltration of the tissues by immune cells, resulting in tissue destruction5.
Kobasa et al.3 conducted comparative virological and functional-genomic analyses of monkeys infected with either a contemporary influenza virus or the 1918 pandemic virus. Influenza virus strains are generally classified by the two proteins that vary most widely among them — haemagglutinin (H) and neuraminidase (N). Both of the viruses in Kobasa and colleagues' study are of the H1N1 type, although they differ in their epidemiological and pathological properties because of variations in their other proteins.
Analysis of the animals infected with the contemporary virus showed that the virus was present in only a small area of the respiratory tract, causing mild symptoms. Gene expression patterns in the cells lining the bronchus confirmed a robust innate immune response soon after infection. This response included the induction of α/β-interferons, cytokines and chemokines, but the expression of each of these proteins was transient and correlated directly with virus levels in the infected tissues. The authors therefore conclude that infection with contemporary H1N1 influenza virus elicits a transient but appropriate activation of innate immune defences that ultimately facilitates clearance of the virus and recovery.
By comparison, the 1918 virus replicated to high levels and spread rapidly throughout the respiratory tract of infected animals. Lung tissue from these animals showed severe damage, including bleeding and infiltration of immune cells, at times well after the peak of virus replication. A whole-genome analysis of gene expression — the first in monkeys infected with the 1918 virus — showed that the virus triggered aberrantly high and sustained expression of genes encoding many proteins involved in the innate immune response, including proinflammatory cytokines and chemokines. So Kobasa et al.3 propose that an aberrant innate immune response to the 1918 influenza virus could be responsible for the rapid, severe outcome of the infection (Fig. 1). Their data suggest that persistent elevation of inflammatory-response genes could account for the massive inflammation and infiltration of immune cells observed in the respiratory tract of animals infected with the 1918 virus.
Notably, infection with contemporary influenza virus triggered robust expression of genes encoding several α-interferon subtypes and of interferon-stimulated genes with known antiviral properties, and this expression was associated with the comparative mildness of the disease. By contrast, the 1918 virus induced only low and selective expression of genes encoding α-interferon subtypes and differential expression of many interferon-stimulated genes — including one that encodes a protein called RIG-I. Contemporary influenza A virus triggers innate immune defences in part through RIG-I, which regulates the expression of other immune and inflammation genes6. But tissue infected with the 1918 virus showed reduced RIG-I expression compared with tissue infected with the contemporary virus. Although these observations may conceptually link RIG-I activity to intracellular pathways that would normally induce the production of interleukins and chemoattractants to clear the virus, the study lacks the biochemical data that are essential for defining such connections.
Kobasa et al.3 demonstrate the power of functional genomics in untangling the complexities of virus–host interactions and viral pathogenesis. Their gene-expression profile analyses suggest that the 1918 virus triggers innate immune signalling processes that possess altered kinetics relative to the contemporary influenza A virus, and/or that the 1918 virus may selectively attenuate the expression of specific innate-response genes. The authors did not, however, analyse gene expression during the important early time course of these events (the first hours and days of infection), so determining the exact mechanisms regulating gene expression will take further study. But it is worth mentioning one possible candidate molecule: the nonstructural-1 (NS1) protein of the influenza A virus can suppress innate immunity by disrupting the induction of α/β-interferon and/or altering the maturation of host-cell RNA7,8. Of course, NS1 does not work alone9, and virulence attributed to pandemic influenza requires many viral components in a unique assemblage of genes1,2.
The work of Kobasa et al. substantiates the findings of Kash et al.10, who showed in mice that the 1918 virus triggered a vigorous innate immune response that was linked to fatalities. Although the mechanisms of tissue destruction were not addressed in either study, the work clearly demonstrates the vital function of early innate immune defences in controlling the virus. It seems that the pandemic 1918 virus had a genetic composition and rapid replication kinetics that may have resulted in an excessively vigorous innate immune and inflammatory response that contributed to severe tissue damage, disease and death.
These conclusions correspond to the striking epidemiological data showing that, unlike contemporary influenza strains, which typically affect the very young and the elderly most severely, the 1918 influenza pandemic was mostly fatal in young adults, who generally possess more robust immune systems1. Unveiling the contribution of an aberrant host response to the pathogenesis of the 1918 virus is just the beginning of efforts to understand the disease mechanisms underlying the 1918 pandemic and new virulent strains of influenza virus. The emergence of the H5N1 avian influenza or 'bird flu' virus, and its transfer to the human population, are real and continuing threats1 that underscore the importance of the current study and of characterizing highly pathogenic forms of flu virus. A better understanding of the origin, transmission and virulence of pandemic influenza viruses, and their interactions with host immune processes, will assist our preparation against future and possibly deadly influenza pandemics.
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Kash, J. C. et al. Nature 443, 578–581 (2006).
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Loo, YM., Gale, M. Fatal immunity and the 1918 virus. Nature 445, 267–268 (2007). https://doi.org/10.1038/445267a
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