Figure 1: Finding the needle in the haystack3,4. | Nature

Figure 1: Finding the needle in the haystack3,4.

From: Catalytic competition for cells

Figure 1

Water-in-oil-in-water emulsions of the cell's protein-synthesis machinery allow fluorescence-activated ‘cell’ sorting (FACS) of individual droplets containing not only a protein and its unique DNA sequence, but also fluorogenic reporters for different chemical transformations. Individual droplets that contain enzyme variants (purple) with increased catalytic activity can be sorted at a rate of about 107 per hour based on the number of fluorescent product molecules (P) synthesized from the substrate (S). The DNA (red) from active droplets can then be recovered and amplified.