Bax causes apoptosis by associating with mitochondria and triggering cytochrome c release, which activates the caspase cascade. Bax can also kill some cells independently of caspases, but the requirements for such killing are poorly understood. Here we describe an inducible fibroblast line that expresses Bax when tetracycline is withdrawn; the resulting apoptosis can be blocked by the caspase inhibitor zVAD-fmk. Even when caspases are inhibited, however, treating the Bax-expressing cells with the mitochondrial toxin oligomycin efficiently triggers death with features resembling apoptosis. Bax mutants lacking the BH3 domain remain able to cause cytochrome c release and caspase-mediated death, but cannot support this caspase-independent killing. Mutating specific BH3 residues needed for binding Bcl2 does not prevent synergy with oligomycin, implying that no such binding is required. These findings illuminate a caspase-independent pathway of death that depends on the Bax BH3 domain and on effectors emanating from mitochondria.
Mitochondria have been implicated as key components of the apoptotic machinery (reviewed in1). Early evidence of their involvement came from the finding that cytochrome c (cyto c), a soluble protein that normally resides between the inner and outer mitochondrial membranes, is released into the cytoplasm in response to many apoptotic stimuli; cyto c release, in turn, leads to activation of caspase-9, which activates the downstream caspase cascade.2,3 Subsequent studies indicate that mitochondria can store and release other apoptogenic factors as well, including certain pro-caspases.4,5,6 A variety of apoptotic signals act through mitochondria: caspase activation itself can trigger cyto c release from these organelles to amplify an ongoing response, but other death signals, including ultraviolet radiation and staurosporine, mobilize cyto c independently of caspases.7 Mitochondria also appear to be the primary locus of action for the Bcl2 family of apoptotic regulatory proteins.8,9 For example, the anti-apoptotic activity of Bcl2 reflects in part its capacity to suppress cyto c release from these organelles.10,11 By contrast, certain pro-apoptotic family members, such as Bax and tBID, are potent inducers of cyto c release, and translocate rapidly from the cytoplasm onto mitochondria soon after apoptotic stimulation.12,13,14,15,16,17,18
Mitochondria thus serve to integrate many different types of death signals and, by virtue of their ability to activate caspase-9, couple these signals to the caspase cascade. There is, however, some evidence that these organelles can access other active cell-death pathways that do not depend on caspase activation. In particular, two groups have reported that overexpressing Bax can induce rapid killing of lymphoid cells even when caspase activity is blocked.19,20 Bax also can kill yeast cells, which express no known caspases.21 In this report, we describe a fibroblast cell line that undergoes caspase-independent death with certain features of apoptosis when exposed to the combination of Bax and the mitochondrial toxin oligomycin. Our findings confirm the existence of a mitochondrially-mediated death pathway that functions independently of caspase activation.
Inducible Bax expression in GM701 fibroblasts
To study the mechanisms of Bax-induced death, we transfected the human GM701 fibroblast cell line with an HA-epitope-tagged mouse Bax cDNA under the control of a tetracycline-repressible promoter. A gene cassette conferring hygromycin resistance was introduced simultaneously, and stably transfected colonies selected first for growth in hygromycin and tetracycline (Tet) were then screened to identify those that died following Tet withdrawal. Our initial analysis utilized one stable clone (designated I-Bax) which demonstrated an especially marked death response when Tet was withdrawn; major findings were later confirmed using transiently transfected populations of the parental cells. As expected, withdrawing Tet from I-Bax cells caused increased expression of both the HA-tagged Bax protein and its mRNA within 24 h, as judged from immunoblots (Figure 1A) and Northern blots (Figure 1B) that specifically detected products of the transgene. This induction was verified by HA-specific immunoprecipitation from biosynthetically radiolabelled cells, which revealed that synthesis of the tagged Bax protein increased markedly as early as 12 h after drug withdrawal (Figure 1C). Even before withdrawal, levels of the tagged protein and mRNA were higher in I-Bax than in GM701 cells that had been transfected with the hygromycin-resistance marker only (Hygro), implying that Tet repression of the Bax gene was incomplete. The I-Bax cells appeared unaffected by this low background expression of the transgene, as their basal rates of growth and their sensitivities to apoptotic stimuli such as staurosporine and ionizing radiation were comparable to those of Hygro or GM701 cells (data not shown). GM701 parental cells contain endogenous Bax (Figure 1D), which suggests that the additional basal expression from the epitope-tagged transgene can be tolerated through countervailing mechanisms that are already present in the cell.
Soon after Tet-withdrawal, the I-Bax cells began to exhibit changes typical of apoptosis. For example, the percentage of cells that bound annexin V (Figure 2A), showed a reduced mitochondrial transmembrane potential (ΔΨm) as assayed using the lipophilic fluorescent dye DiOC6 (Figure 2B), or failed to exclude trypan blue (data not shown) increased significantly within 24 h after drug withdrawal. Caspase activation could be detected as early as 12 h post-withdrawal, as evidenced by cleavage of endogenous PARP protein (Figure 2C). Although the percentage of cells actively undergoing apoptosis as measured by these assays peaked at 20–25% after 72 h, the cumulative effect of the ongoing deaths was more apparent in the growth kinetics of the population, in that net proliferation of the cells was reduced by more than 70% after 3 days without Tet (Figure 2D). The rate of apoptosis was further augmented if the cells were passaged during this time, so that no viable cells remained after two passages without Tet over 5–6 days (data not shown).
Caspases and Bax-induced apoptosis
The apoptosis of Tet-deprived I-Bax cells was fully inhibitable by zVAD-fmk, a broad-spectrum caspase inhibitor. As illustrated in Figure 3A, we found that adding 40 μM zVAD-fmk to the culture medium at the time of Tet withdrawal completely suppressed the apoptotic response, as assayed in flow cytometric assays for surface annexin V binding, for ΔΨm using DiOC6, or for nuclear DNA content. In each of these assays, the percentage of apoptotic cells at 72 h post-withdrawal was reduced from 22–25% in the absence of zVAD-fmk to 3–6% in its presence (Figure 3A); the latter values are indistinguishable from background levels seen prior to Tet withdrawal or in parental GM701. Comparable inhibition could also be achieved by treating the cells with a combination of two different peptide inhibitors (at 40 μM each) that together inhibit caspases 8 and 9 selectively. Using a more sensitive FACS assay based on the fluorescent dye JC-1, we were able to detect a subtle effect of Bax on mitochondrial function which persisted despite caspase inhibition: 24 h of Tet withdrawal alone caused a significant loss of ΔΨm (reflected in a decrease in red emission from JC-1 dimers), which was essentially unaffected by zVAD-fmk (Figure 3B).
Bax effects on the mitochondria
To verify the interaction of Bax with mitochondria in the presence of caspase inhibitors, we utilized indirect immunofluorescence with a primary antiserum directed specifically against the HA-epitope tag on the transgenic Bax. All cells were additionally stained with a second primary antiserum that recognized cyto c. In I-Bax cells growing in Tet, cyto c was present within mitochondria, and no HA-tagged Bax was detectable, presumably because it was scarce and dispersed throughout the cytosol (data not shown). After 24 h of Tet withdrawal in the presence of zVAD-fmk (Figure 4), HABax immunoreactivity (red) was detected in two distinct patterns. In certain cells, HABax colocalized with intramitochondrial cyto c (green) in a dispersed punctate pattern (Figure 4, upper panels); in the majority of cells, HABax-positive mitochondria were found aggregated in a dense perinuclear cluster, but failed to stain for cyto c, which had presumably been discharged into the cytosol (Figure 4, lower panels). The identity of the clustered organelles was confirmed by staining with MitoTracker green, a mitochondrion-specific dye (data not shown). The latter staining pattern was seen in 64% of the HA positive cells at 24 h, and 79% of the positive cells at 72 h after-Tet withdrawal. This temporal progression suggests that the newly-synthesized HABax protein rapidly associates with mitochondria, which subsequently release cyto c and cluster near the nucleus without the requirement of caspase activation. This confirms earlier reports15,22 which also found that blocking caspase activation using zVAD-fmk did not prevent Bax- or Bid-induced release of cyto c. Thus, when caspases are inhibited, Bax can cause some perturbations of mitochondrial function in these cells, but fails to induce apoptosis.
Caspase-independent death induced by oligomycin together with Bax
We then asked whether other substances that targeted the mitochondria could function synergistically with Bax. In particular, we tested the effects of two potent mitochondrial toxins–oligomycin and antimycin–which inhibit the F1F0-adenosine triphosphatase (-ATPase) and complex III of the electron transport chain, respectively. Typical results are shown in Figure 5. At the concentrations tested, each agent by itself produced little or no increase in death of I-Bax cells growing in Tet, or of Hygro cells, as measured by surface annexin V binding and by staining with propidium iodide (Figure 5A,B). However, each toxin greatly increased the death response that occurred after Tet withdrawal in I-Bax cells, indicating that each could augment the effect of Bax. The death response induced by the combination of Bax and antimycin could be almost completely suppressed by treatment with zVAD-fmk. Remarkably, however, zVAD-fmk yielded only a partial decrease in the response to Bax and oligomycin, so that a substantial rate of cell death was induced with this combination despite caspase blockade (Figure 5A,B). A similar effect was seen in mitochondria, where the combination of Tet withdrawal and oligomycin was uniquely able to provoke a loss of ΔΨm as measured using DiOC6, despite treatment with zVAD-fmk (Figure 5C). Even the combination of zVAD-fmk with selective inhibitors of caspases 8 and 9 failed to prevent this cell death induced by Bax and oligomycin, although it appeared to increase the number of necrotic cells (Figure 5D, upper right quadrant), as well as the larger proportion of cells which appear apoptotic (Figure 5D, lower right quadrant). These findings therefore define a caspase-independent pathway of cell death that yields many of the characteristic changes of apoptosis and is triggered by the combined effects of oligomycin and Bax.
Role of the Bax BH3 domain
Several studies have suggested that Bax and other proapoptotic members of the Bcl2 family have both BH3-dependent and independent effects.16,22,23 We therefore asked whether the BH3 domain of Bax was required for its ability to cooperate with oligomycin in inducing caspase-independent death. To address this question, we constructed a series of plasmids encoding HA-tagged chimeric proteins (Figure 6A) formed from selected regions of Bax (filled bars) and Bcl2 (open bars). The ability of these proteins to induce apoptosis independently of oligomycin was tested by transfecting each plasmid transiently into GM701 cells along with a reporter plasmid encoding β-galactosidase (β-gal), then scoring the percentage of β-gal-positive cells that showed morphologic features of apoptosis 18 h later.24 As shown in Figure 6B, Bax itself induces apoptosis in approximately 55% of cells in this assay, well above the background level obtained with Bcl2. We previously reported24 that a chimera termed C10, in which the BH3 domain of Bcl2 is replaced by that of Bax, also induces apoptosis efficiently. For this study, we created four additional chimeras in which progressively shorter sequences in and around the BH3 region from Bcl2 replaced the corresponding sequences in Bax; two of these (SW-3 and SW-4) contained the complete Bax BH3 domain, but the other two (SW-1 and SW-2) did not. All of the latter chimeras had proapoptotic activities comparable to that of Bax itself, except for SW-1, which was roughly half as active (Figure 6B).
We then assayed which of these mutants could synergize with oligomycin to induce caspase-independent death (Figure 6C). Each construct was transiently transfected into GM701 cells along with the β-gal reporter; 6 h later, zVAD-fmk was added to the culture medium either with or without oligomycin, and apoptosis-like morphologic changes were scored at 30 h after transfection. The background level of such changes was relatively high (11–15% of cells) in these studies, presumably owing to the transient transfection itself and to the delayed addition of zVAD-fmk; this background was reduced (to 8%) in the cells receiving Bcl2. Importantly, under these conditions, the addition of oligomycin significantly increased the rate of apoptosis-like morphology induced by Bax and by C10, but not by Bcl2. Of the remaining chimeras, SW-3 and SW-4 were likewise able to synergize with oligomycin, but SW-1 and SW-2 did not. This suggested that residues critical for this synergy are located between positions 61 and 68 of Bax, a region that overlaps the BH3 domain (residues 63–71). Together, these results indicate that an intact Bax BH3 domain is critical for activating caspase–independent death in cooperation with oligomycin.
Structural requirements for other mitochondrial effects
To determine if any of the other effects we had observed in mitochondria could be mapped to particular domains in Bax, we performed immunofluorescence analyses in GM701 cells transiently transfected with the various Bax/Bcl2 chimeras. Representative results are shown in Figure 7. By 24 h post-transfection, wild-type Bax, as well as chimeras SW1 through SW4 had localized to mitochondria and induced cyto c release, as judged by a lack of costaining with the cyto c antibody (Figure 7 and data not shown). Because SW1 and SW2 each lack all or part of the Bax BH3 domain, these findings confirm earlier evidence that this domain is not required for mitochondrial localization.22
Interestingly, unlike the other chimeras, SW1 clearly failed to cause clumping of mitochondria (Figure 7), suggesting that Bax residues at positions 68–76 might be required for this effect.
BH3 point mutants and caspase-independent death
We also tested three missense mutants of Bax, each of which contained alanines in place of two consecutive residues within the BH3 domain (Figure 8A). When transiently expressed in GM701 cells, all three mutants induced apoptosis at least as efficiently as wild-type Bax (data not shown). Moreover, all three associated with mitochondria and induced both clumping (Figure 8B and data not shown) and cyto c release. However, only one mutant (BaxDE) was capable of synergizing with oligomycin to induce death in the presence of zVAD-fmk (Figure 8A). As the latter mutation involved two residues that are each specifically required for the BH3-mediated dimerization of Bax with Bcl2,25 this implies that no such interaction is needed to support caspase-independent death.
We have created and characterized a stably transfected cell line, derived from human GM701 fibroblasts, that inducibly expresses the proapoptotic protein Bax. When Bax is induced, these cells undergo a classic apoptotic response that can be completely inhibited by pharmacologically blocking caspase activation. This strict caspase-dependence is compatible with the view that Bax, like other BH3-containing death agonists, acts primarily by inducing release of cyto c or other factors from mitochondria, which lead to activation of upstream caspases, and then to the propagation of apoptosis through activation of downstream caspases.1,2 Surprisingly, however, we find that treatment with oligomycin–a mitochondrial toxin which, at the dosage we used, fails to cause apoptosis by itself–efficiently triggers death of the Bax-expressing cells even when caspases are inhibited. Cells dying under the latter conditions exhibit several of the defining features of apoptosis, including surface annexin V binding, cytoplasmic shrinkage, and mitochondrial dysfunction. These findings verify the existence of a robust, caspase-independent pathway of death resembling apoptosis, and they provide an experimental model system in which to study this pathway in isolation. They also imply that a key element of this pathway emanates from mitochondria.
Our results confirm and complement earlier reports19,20 of caspase-independent death caused by Bax. In those studies, which employed the T-lymphocyte line Jurkat, overexpressing Bax alone was sufficient to cause rapid cell death that occurred despite treatment with zVAD-fmk at dosages that effectively blocked both Bax-induced caspase activation and Fas-mediated apoptosis. This suggests that, in Jurkat cells, Bax simultaneously activates two parallel pathways–one caspase-dependent, the other caspase-independent–that can each lead independently to death. By contrast, our study indicates that, in GM701 fibroblasts, Bax alone triggers only caspase-dependent death, whereas caspase-independent killing requires both Bax and a second signal that can be supplied by oligomycin. A likely interpretation is that there are important tissue-specific differences in regulation of the two pathways. Consistent with that view, earlier studies of caspase-9-deficient knockout mice found that thymocytes from such animals were sensitive to radiation-induced apoptosis but that embryonic fibroblasts were not, suggesting that T cells are better able to access an alternative death pathway.26,27
Our data extend earlier results by showing that the BH3 domain in Bax is strictly required for its ability to activate caspase-independent death in cooperation with oligomycin. This is in contrast to other properties of Bax, such as mitochondrial localization and the induction of cyto c release, which do not require an intact BH3 region. Bax's role in activating the second pathway may thus reflect its ability to lower ΔΨm, which is known to be BH3-dependent,22 or to form ion channels in cellular membranes.28,29 In our cells, induction of wild-type Bax causes mitochondria to aggregate in a perinuclear region of the cytoplasm; our mutational analysis maps this clumping response to residues in, or immediately downstream of, the BH3 domain. Li et al.15 have previously noted mitochondrial clumping during the apoptotic response to tBID, another death agonist that contains a BH3 domain. The mechanism of this BH3-dependent clumping is unclear: as similar clumping can also be produced by genetic defects in microtubules,30 it may result from selective detachment of mitochondria away from the microtubular network, or from destruction of microtubules themselves in apoptotic cells. Our data indicate that Bax-induced mitochondrial clumping can occur when caspases are inhibited, but they do not reveal whether such clumping contributes to caspase-independent killing. Significantly, however, we find that another known function of the Bax BH3 domain–the ability to associate physically with Bcl2–is probably not necessary for activation of this alternative pathway: although previous studies have shown that replacing Bax BH3 residues 68 or 69 individually with alanine prevents binding to Bcl2 in vitro, we find that mutating both these residues simultaneously does not prevent the caspase-independent response (Figure 8A).
Oligomycin acts on the F1F0-ATPase of the mitochondrial inner membrane, which utilizes the energy stored in the transmembrane proton gradient to convert ADP to ATP. By blocking this enzyme, oligomycin prevents dissipation of the proton gradient and causes mitochondrial hyperpolarization. A functional linkage between Bax and F1F0-ATPase was previously suggested on genetic grounds, based on a study of Bax-induced killing in yeast,31 but its biochemical basis remains uncertain. The fact that certain point mutants of Bax lose the oligomycin effect we describe suggests that it may be a specific protein-protein interaction between Bax and a component of the mitochondria that is responsible for caspase-independent cell death. Recent studies suggest that Bax binds and inhibits the mitochondrial ATP/ADP antiporter, which pumps ATP out of the organelle in exchange for ADP.32 It may be that, by blocking import of ADP, Bax reduces the availability of this F1F0-ATPase substrate, and so enhances vulnerability to oligomycin. Consistent with this model, Bcl-XL, an antagonist of Bax, prevents oligomycin-induced hyperpolarization of mitochondria.33 Bax has also been shown to bind to the mitochondrial voltage dependent anion channel (VDAC) and enable the passage of cyto c through this pore.34 In our system, cyto c release and cooperation with oligomycin appear to map to different regions of Bax, suggesting that Bax could potentially interact with multiple components of the mitochondria and cause the release of several factors that each activate separate death pathways. In this regard, Susin et al.5 have recently reported purification of apoptosis inducing factor (AIF), a 57-kilodalton flavoprotein that normally resides in the mitochondrial intermembranous space but can be released to the cytoplasm in response to apoptotic stimuli. Once released, AIF potently induces chromatin condensation, large-scale chromosomal fragmentation, loss of ΔΨm, and exposure of surface phosphatidylserine; most significantly, these activities are all resistant to inhibition by zVAD-fmk. The effects of AIF, which closely mirror those we observed in oligomycin/zVAD-fmk treated I-Bax cells, illustrate that many of the classic features of apoptosis can occur without caspase activation. It remains to be determined whether it is AIF itself or some other, as-yet-unidentified mitochondrial products that mediate caspase-independent killing of these cells.
Materials and Methods
The human GM701 fibroblast line and all clones derived from it were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with penicillin (100 U/ml), streptomycin (0.1 mg/ml), glutamate (2 mM) and 10% (v/v) fetal calf serum. All cells were maintained at 37°C in 5% CO2 at 90% relative humidity. To establish Bax-inducible lines, GM701 cells were first transfected by electroporation with pTET-Off regulatory plasmid (Clontech PT 3001-1), and a stable clone was selected according to the manufacturer's protocol. The pTRE-HABax response plasmid was constructed by inserting a murine bax cDNA with an N-terminal influenza hemagglutinin (HA) epitope tag (YPYDVPDYA) into the EcoRI-XbaI sites of pTRE (Clontech). pTRE-HABax was transfected into the stable pTET-Off line along with a pTK-hygromycin selection plasmid. Stable transfectants were selected in 5 μg/ml tetracycline (Tet) and 0.2 mg/ml hygromycin, and then screened for cell death upon withdrawal of Tet. All Tet-dependent clones were supplemented with 5 μg/ml Tet every 1–2 days. To remove Tet, cells were washed in phosphate-buffered saline (PBS), trypsinized (0.25%), and resuspended in PBS, pelleted at 1000×g for 5 min, and then replated in fresh media. zVAD-fmk and other caspase inhibitors were used at 40 μM each, and were replenished daily whenever used for longer than 24 h.
Rhodamine-conjugated anti-HA (12CA5) and FITC-conjugated goat anti-mouse sera were purchased from Boehringer Mannheim. Anti-human PARP (clone C2-10) and anti-cytochrome c (7H8.2C12) monoclonal antibodies were purchased from Pharmingen. Anti-Bax polyclonal antibody (P-19) was purchased from Santa Cruz Biotechnology. Antibodies were used at dilutions recommended by the suppliers. Propidium iodide, oligomycin, antimycin, and saponin were from Sigma; tetracycline, hygromycin, caspase-8 inhibitor I (IETD-CHO), caspase-9 inhibitor II (LEHD-CHO), and annexin V-FITC were from Calbiochem; zVAD-fmk was from Alexis Laboratories; DiOC6, JC-1, and Mitotracker Green were from Molecular Probes.
Western blot analysis
Cells were lysed in Triton-X lysis buffer (150 mM NaCl, 10 mM Tris, pH 7.4, 5 mM EDTA, 1% [v/v] Triton X-100, 5 μg/ml leupeptin, 1 μg/ml pepstatin, and 0.5 mM Pefabloc [Boehringer Mannheim]). Aliquots containing 30–50 μg protein were fractionated by SDS-polyacrylamide gel electrophoresis (PAGE) and transferred to Hybond filters (Amersham). Filters were pre-blocked for 15 min in Blotto (PBS with 3% [v/v] Carnation milk, 0. 1% [v/v] Tween-20), then incubated for 1 h with primary antibodies in Blotto. Filters were then washed three times in PBS with 0.1% Tween-20 for 10 min each, incubated with secondary antisera in Blotto for 1 h, washed again three times, and developed using ECL reagent (Pierce).
RNA preparation and Northern blot analysis
RNA was isolated using the Trizol reagent (GibcoBRL). Aliquots (10 μg) were fractionated on formaldehyde/agarose gels, which were then soaked in water for 20 min and then soaked for an additional 15 min in 20× standard saline citrate (SSC, 1×SSC is 150 mM NaCl, 15 mM sodium citrate, pH 7.0). RNA was transferred overnight onto Hybond paper (Amersham) by capillary action, and crosslinked to it with ultraviolet light. Hybridization was done using radiolabelled DNA probes in ExpressHyb (Clontech) according to manufacturer's instructions, and detected by autoradiography. The HABax probe was a unique KpnI/BglII fragment of pTRE-HABax. Glyceraldehyde 6-phosphate dehydrogenase (GAPDH) mRNA was detected with a PstI/HindIII fragment of GAPDH cDNA.
Six hours before harvest, the media of the cells was changed to methionine-free DMEM containing 10% dialyzed fetal calf serum, 100 μCi/ml of 35S-methionine (Amersham). Cells were harvested in Triton-X lysis buffer, and lysates were precleared overnight with 30 μl of ProteinA/G-Plus Agarose beads (Santa Cruz Biologicals). The following day, antibody was added and incubated for 3 h at room temperature, then 30 μl agarose beads were added for 3 h. Beads were washed three times in Triton-X lysis buffer, then eluted in 1× SDS sample buffer. Samples were fractionated on 14% PAGE gels, which were then fixed, treated for 30 min with AmplifyTM (Amersham), and analyzed by autoradiography.
Assessment of apoptosis by fluorescence activated cell sorting (FACS)
Both floating and adherent cells were analyzed in all assays. For annexin V binding, cells were harvested using trypsin and then incubated with annexin V-FITC according to the manufacturer's instructions. For determination of DNA content, cells were harvested using trypsin, washed in PBS, pelleted, resuspended in 500 μl PBS, and then fixed by dropwise addition of 3 ml ice-cold 70% ethanol. After fixing for at least 30 min on ice, cells were washed in PBS, resuspended in PBS containing 20 μg/ml ribonuclease A and 20 μg/ml propidium iodide, and then incubated for 15 min at room temperature before analysis. For analysis of mitochondrial function, either 50 nM DiOC6 or 10 μg/ml JC-1 dye was added to the media, and cells were harvested by trypsin 15 min later and resuspended in PBS and analyzed immediately. All samples were analyzed using a Becton-Dickinson FACSort and Cellquest software, examining a minimum of 10 000 cells in each sample.
Transient transfection studies
To create chimeras SW1-SW4, a HindIII restriction site was introduced into basepairs 135-138 of a pSFFV-HABax/β-galactosidase expression plasmid,24 resulting in missense mutation of threonine to serine at Bax residue 46. Fragments of bcl2 were cloned into bax by PCR using this HindIII site and the PstI site at basepair 226. Double alanine mutations were also introduced by PCR. Transient transfections were performed as described.24 Where indicated, zVAD-fmk and oligomycin were added 6 h after transfection.
I-Bax cells treated as described or GM701 cells transfected as described were grown on glass cover slips, and fixed in 2% paraformaldehyde in PBS. Cells were washed in PBS with 0.03% saponin, then permeablized in PBS with 0.1% saponin and 1% BSA. Cells were double-labeled first with anti-cytochrome c at 1:75 dilution and FITC-conjugated goat anti-mouse IgG at 1:50 dilution, and then with rhodamine-conjugated anti-HA at 1:20 dilution. DAPI was added in the mounting medium (Vectashield, Vector Laboratories). Cells were examined using a Nikon Eclipse TE300 microscope and IP Lab Spectrum software (Scanalytics, Inc.) To quantify ‘clumping,’ I-Bax cells were deprived of Tet and treated with 40 μM zVAD-fmk. After 24 or 72 h, the cells were fixed and probed as described above, and 300 HA-positive cells were examined in each case. In general, all HA-positive mitochondria that were clumped were also negative for cyto c (see Figure 4, lower panel), whereas those found dispersed in the cytoplasm were variably either positive or negative for intramitochondrial cyto c.
- cyto c:
mitochondrial transmembrane potential
fluorescence activated cell sorting
glyceraldehyde 6-phosphate dehydrogenase
polyacrylamide gel electrophoresis
poly(ADP ribosyl) polymerase
Green DR and Reed JC. . (1998) Mitochondria and apoptosis. Science 281: 1309–1312
Liu X, Kim CN, Yang J, Jemmerson R and Wang X. . (1996) Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c. Cell. 86: 147–157
Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES and Wang X. . (1997) Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 91: 479–489
Susin SA, Zamzami N, Castedo M, Hirsch T, Marchetti P, Macho A, Daugas E, Geuskens M and Kroemer G. . (1996) Bcl-2 inhibits the mitochondrial release of an apoptogenic protease. J. Exp. Med. 184: 1331–1341
Susin SA, Lorenzo HK, Zamzami N, Marzo I, Snow BE, Brothers GM, Mangion J, Jacotot E, Costantini P, Loeffler M, Larochette N, Goodlett DR, Aebersold R, Siderovski DP, Penninger JM and Kroemer G. . (1999) Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 397: 441–446
Susin SA, Lorenzo HK, Zamzami N, Marzo I, Brenner C, Larochette N, Prevost MC, Alzari PM and Kroemer G. . (1999) Mitochondrial release of caspase-2 and -9 during the apoptotic process. J. Exp. Med. 189: 381–394
Bossy-Wetzel E, Newmeyer DD and Green DR. . (1998) Mitochondrial cytochrome c release in apoptosis occurs upstream of DEVD-specific caspase activation and independently of mitochondrial transmembrane depolarization. EMBO J. 17: 37–49
Adams JM and Cory S. . (1998) The Bcl-2 protein family: arbiters of cell survival. Science 281: 1322–1326
Chao DT and Korsmeyer SJ. . (1998) BCL-2 family: regulators of cell death. Annu. Rev. Immunol. 16: 395–419
Kluck RM, Bossy-Wetzel E, Green DR and Newmeyer DD. . (1997) The release of cytochrome c from mitochondria: a primary site for Bcl-2 regulation of apoptosis. Science 275: 1132–1136
Yang J, Liu X, Bhalla K, Kim CN, Ibrado AM, Cai J, Peng TI, Jones DP and Wang X. . (1997) Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked. Science 275: 1129–1132
Hsu YT, Wolter KG and Youle RJ. . (1997) Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis. Proc. Natl. Acad. Sci. USA. 94: 3668–3672
Wolter KG, Hsu YT, Smith CL, Nechushtan A, Xi XG and Youle RJ. . (1997) Movement of Bax from the cytosol to mitochondria during apoptosis. J. Cell. Biol. 139: 1281–1292
Jurgensmeier JM, Xie Z, Deveraux Q, Ellerby L, Bredesen D and Reed JC. . (1998) Bax directly induces release of cytochrome c from isolated mitochondria. Proc. Natl. Acad. Sci. USA. 95: 4997–5002
Li H, Zhu H, Xu CJ and Yuan J. . (1998) Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis. Cell 94: 491–501
Luo X, Budihardjo I, Zou H, Slaughter C and Wang X. . (1998) Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors. Cell. 94: 481–490
Narita M, Shimizu S, Ito T, Chittenden T, Lutz RJ, Matsuda H and Tsujimoto Y. . (1998) Bax interacts with the permeability transition pore to induce permeability transition and cytochrome c release in isolated mitochondria. Proc. Natl. Acad. Sci. USA. 95: 14681–14686
Rosse T, Olivier R, Monney L, Rager M, Conus S, Fellay I, Jansen B and Borner C. . (1998) Bcl2 prolongs cell survival after Bax induced release of cytochrome c. Nature 391: 496–499.
Xiang J, Chao DT and Korsmeyer SJ. . (1996) BAX-induced cell death may not require interleukin 1 beta-converting enzyme-like proteases. Proc. Natl. Acad. Sci. USA. 93: 14559–14563
Pastorino JG, Chen ST, Tafani M, Snyder JW and Farber JL. . (1998) The overexpression of Bax produces cell death upon induction of the mitochondrial permeability transition. J. Biol. Chem. 273: 7770–7775
Zha H, Fisk HA, Yaffe MP, Mahajan N, Herman B and Reed JC. . (1996) Structure-function comparisons of the proapoptotic protein Bax in yeast and mammalian cells. Mol. Cell Biol. 16: 6494–6508
Han J, Modha D and White E. . (1998) Interaction of E1B 19K with Bax is required to block Bax-induced loss of mitochondrial membrane potential and apoptosis. Oncogene 17: 2993–3005
Chou JJ, Li H, Salvesen GS, Yuan J and Wagner G. . (1999) Solution structure of BID, an intracellular amplifier of apoptotic signaling. Cell 96: 615–624
Hunter JJ and Parslow TG. . (1996) A peptide sequence from Bax that converts Bcl-2 into an activator of apoptosis. J. Biol. Chem. 271: 8521–8524
Zha H and Reed JC. . (1997) Heterodimerization-independent functions of cell death regulatory proteins Bax and Bcl-2 in yeast and mammalian cells. J. Biol. Chem. 272: 31482–31488
Hakem R, Hakem A, Duncan GS, Henderson JT, Woo M, Soengas MS, Elia A, de la Pompa JL, Kagi D, Khoo W, Potter J, Yoshida R, Kaufman SA, Lowe SW, Penninger JM and Mak TW. . (1998) Differential requirement for caspase 9 in apoptotic pathways in vivo. Cell 94: 339–352
Kuida K, Haydar TF, Kuan CY, Gu Y, Taya C, Karasuyama H, Su MS, Rakic P and Flavell RA. . (1998) Reduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase 9. Cell 94: 325–337
Antonsson B, Conti F, Ciavatta A, Montessuit S, Lewis S, Martinou I, Bernasconi L, Bernard A, Mermod JJ, Mazzei G, Maundrell K, Gambale F, Sadoul R and Martinou JC. . (1997) Inhibition of Bax channel-forming activity by Bcl-2. Science 277: 370–372
Schlesinger PH, Gross A, Yin XM, Yamamoto K, Saito M, Waksman G and Korsmeyer SJ. . (1997) Comparison of the ion channel characteristics of proapoptotic BAX and antiapoptotic BCL-2. Proc. Natl. Acad. Sci. USA. 94: 11357–11362
Tanaka Y, Kanai Y, Okada Y, Nonaka S, Takeda S, Harada A and Hirokawa N. . (1998) Targeted disruption of mouse conventional kinesin heavy chain, kif5B, results in abnormal perinuclear clustering of mitochondria. Cell 93: 1147–1158
Matsuyama S, Xu Q, Velours J and Reed JC. . (1998) The Mitochondrial F0F1-ATPase proton pump is required for function of the proapoptotic protein Bax in yeast and mammalian cells. Mol. Cell. 1: 327–336
Marzo I, Brenner C, Zamzami N, Jurgensmeier JM, Susin SA, Vieira HL, Prevost MC, Xie Z, Matsuyama S, Reed JC and Kroemer G. . (1998) Bax and adenine nucleotide translocator cooperate in the mitochondrial control of apoptosis. Science 281: 2027–2031
Vander Heiden MG, Chandel NC, Williamson EK, Schumacker PT and Thompson CB. . (1997) Bcl-xL regulates the membrane potential and volume homeostasis of mitochondria. Cell. 91: 627–637
Shimizu S, Narita M and Tsujimoto Y. . (1999) Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC. Nature 399: 483–487
This work was supported in part by NIH grants AI36636 and AI40317, and by the Barbara Fasken Memorial Fellowship from the Prairie Foundation. We thank Zeneida Mosquera for technical assistance.
Edited by D Green
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Fitch, M., Chang, CM. & Parslow, T. The BH3 domain is required for caspase-independent cell death induced by Bax and oligomycin. Cell Death Differ 7, 338–349 (2000). https://doi.org/10.1038/sj.cdd.4400659
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