Abstract
DNA fragmentation is a hallmark of apoptosis, and has been viewed as a short-lived process (<hour) that immediately precedes cell elimination. However, use of sensitive techniques like in situ end-labelling plus (ISEL+) has indicated that the period between the initiation of detectable fragmentation and cell elimination could be longer (days). To address this possibility, we used a model system of cell death and replacement, the murine small intestinal villus. Pulses of 5-bromo-2′-deoxyuridine were used to follow quantitatively cohorts of cells from their generation in the crypts to their elimination at the villus tips, resulting in a temporal `yard-stick' where position on the villus indicated time before cell elimination; these data allowed a mathematical description of cell movement and clearance. Combining these data with ISEL+ quantitation, enterocytes were found to commence and maintain DNA fragmentation 2–3 days before elimination, a phenomenon that likely has relevance to studies on apoptosis also in other systems.
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Edited by J. Cidlowski
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Pompeiano, M., Hvala, M. & Chun, J. Onset of apoptotic DNA fragmentation can precede cell elimination by days in the small intestinal villus. Cell Death Differ 5, 702–709 (1998). https://doi.org/10.1038/sj.cdd.4400403
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DOI: https://doi.org/10.1038/sj.cdd.4400403